Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA.

Abstract:

:The complete nucleotide sequence of the genome of classical swine fever virus (CSFV) strain Alfort/187 was determined from three cDNA libraries constructed by cloning of DNA fragments obtained from independent sets of reverse transcription and PCR. The cDNA fragments were then assembled and inserted downstream of a T7 promoter in a P15A-derived plasmid vector to obtain the full-length cDNA clone pA187-1. The first nucleotide of the CSFV genome was positioned at the transcription start site of the T7 promoter. Cleavage at an SrfI restriction site introduced at the exact 3' end of the cloned viral cDNA allowed the in vitro synthesis of full-length viral RNA by runoff transcription. This RNA proved to be infectious after transfection into porcine kidney cells. Infectivity was not increased after capping of the synthetic RNA. Virus recovered from transfected cells was titrated in porcine kidney cells by endpoint dilution using indirect immunofluorescence and a CSFV-specific monoclonal antibody. RNA transcripts generated from plasmid DNA isolated from bacteria which had been cultured and cloned 10 times remained infectious, indicating that the full-length clone is stable in bacterial cells. A silent point mutation introduced at position 11842 of the genome was retained in the recombinant virus recovered from transfected cells. An infectious chimeric construct was obtained by replacing a 696-bp fragment in pA187-1 with the corresponding cDNA fragment from the CSFV strain CAP. The stably cloned full-length CSFV cDNA allows site-specific mutagenesis of the viral genome and thus will be useful for detailed molecular characterization of the virus as well as for studies of viral pathogenesis.

journal_name

J Virol

journal_title

Journal of virology

authors

Ruggli N,Tratschin JD,Mittelholzer C,Hofmann MA

doi

10.1128/JVI.70.6.3478-3487.1996

subject

Has Abstract

pub_date

1996-06-01 00:00:00

pages

3478-87

issue

6

eissn

0022-538X

issn

1098-5514

journal_volume

70

pub_type

杂志文章
  • Functional phenotype of transformed human alphabeta and gammadelta T cells determined by different subgroup C strains of herpesvirus Saimiri.

    abstract::Based on sequence divergence in the transformation-relevant region, herpesvirus saimiri strains are classified into three subgroups. Only members of subgroup C transform human T lymphocytes to continuous interleukin-2-dependent growth in culture. In this study, human cord blood T cells were immortalized by using diffe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.3.2252-2263.1997

    authors: Fickenscher H,Bökel C,Knappe A,Biesinger B,Meinl E,Fleischer B,Fleckenstein B,Bröker BM

    更新日期:1997-03-01 00:00:00

  • The Host DHX9 DExH-Box Helicase Is Recruited to Chikungunya Virus Replication Complexes for Optimal Genomic RNA Translation.

    abstract::Beyond their role in cellular RNA metabolism, DExD/H-box RNA helicases are hijacked by various RNA viruses in order to assist replication of the viral genome. Here, we identify the DExH-box RNA helicase 9 (DHX9) as a binding partner of chikungunya virus (CHIKV) nsP3 mainly interacting with the C-terminal hypervariable...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01764-18

    authors: Matkovic R,Bernard E,Fontanel S,Eldin P,Chazal N,Hassan Hersi D,Merits A,Péloponèse JM Jr,Briant L

    更新日期:2019-02-05 00:00:00

  • An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice.

    abstract::Intramuscular administration of inactivated influenza virus vaccine is the main vaccine platform used for the prevention of seasonal influenza virus infection. In clinical trials, inactivated H5N1 vaccines have been shown to be safe and capable of eliciting immune correlates of protection. However, the H5N1 vaccines a...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00596-10

    authors: Lau YF,Tang LH,McCall AW,Ooi EE,Subbarao K

    更新日期:2010-09-01 00:00:00

  • Mutations in herpes simplex virus type 1 genes encoding VP5 and VP23 abrogate capsid formation and cleavage of replicated DNA.

    abstract::The herpes simplex virus type 1 capsid is composed of seven capsid proteins which are termed VP5, VP19c, VP21, VP22a, VP23, VP24, and VP26. Major capsid protein VP5 is encoded by the gene UL19. UL18, whose transcript is 3' coterminal with that of VP5, specifies capsid protein VP23. Vero cell lines have been isolated t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.3.1357-1364.1993

    authors: Desai P,DeLuca NA,Glorioso JC,Person S

    更新日期:1993-03-01 00:00:00

  • Glycan Profiles of gp120 Protein Vaccines from Four Major HIV-1 Subtypes Produced from Different Host Cell Lines under Non-GMP or GMP Conditions.

    abstract::Envelope (Env) glycoprotein of human immunodeficiency virus type 1 (HIV-1) is an important target for the development of an HIV vaccine. Extensive glycosylation of Env is an important feature that both protects the virus from antibody responses and serves as a target for some highly potent broadly neutralizing antibod...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01968-19

    authors: Wang S,Voronin Y,Zhao P,Ishihara M,Mehta N,Porterfield M,Chen Y,Bartley C,Hu G,Han D,Wells L,Tiemeyer M,Lu S

    更新日期:2020-03-17 00:00:00

  • The immediate-early gene product encoded by open reading frame 57 of herpesvirus saimiri modulates gene expression at a posttranscriptional level.

    abstract::The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.1.857-861.1998

    authors: Whitehouse A,Cooper M,Meredith DM

    更新日期:1998-01-01 00:00:00

  • Molecular dissection of influenza virus nucleoprotein: deletion mapping of the RNA binding domain.

    abstract::Influenza virus nucleoprotein (NP) is associated with the genome RNA, forming ribonucleoprotein cores. To identify the amino acid sequence involved in RNA binding, we performed Northwestern blot analysis with a set of N- and C-terminal deletion mutants of NP produced in Escherichia coli. The RNA binding region has bee...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.12.8433-8436.1994

    authors: Kobayashi M,Toyoda T,Adyshev DM,Azuma Y,Ishihama A

    更新日期:1994-12-01 00:00:00

  • Role of the alpha/beta interferon response in the acquisition of susceptibility to poliovirus by kidney cells in culture.

    abstract::Replication of poliovirus (PV) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to PV infection after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.80.9.4313-4325.2006

    authors: Yoshikawa T,Iwasaki T,Ida-Hosonuma M,Yoneyama M,Fujita T,Horie H,Miyazawa M,Abe S,Simizu B,Koike S

    更新日期:2006-05-01 00:00:00

  • Conserved Tryptophan Motifs in the Large Tegument Protein pUL36 Are Required for Efficient Secondary Envelopment of Herpes Simplex Virus Capsids.

    abstract:UNLABELLED:Herpes simplex virus (HSV) replicates in the skin and mucous membranes, and initiates lytic or latent infections in sensory neurons. Assembly of progeny virions depends on the essential large tegument protein pUL36 of 3,164 amino acid residues that links the capsids to the tegument proteins pUL37 and VP16. O...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03167-15

    authors: Ivanova L,Buch A,Döhner K,Pohlmann A,Binz A,Prank U,Sandbaumhüter M,Bauerfeind R,Sodeik B

    更新日期:2016-05-12 00:00:00

  • Structural protein markers in the avian oncoviruses.

    abstract::The proteins of purified avian oncoviruses were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and isoelectric focusing. Certain members of the avian leukosis-sarcoma viruses (ALSV) had group-specific antigens with altered electrophoretic properties. (i) The p27 protein of Rous-associated ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.24.3.850-864.1977

    authors: Rettenmier CW,Hanafusa H

    更新日期:1977-12-01 00:00:00

  • Cullin4A and cullin4B are interchangeable for HIV Vpr and Vpx action through the CRL4 ubiquitin ligase complex.

    abstract:UNLABELLED:Human immunodeficiency virus (HIV) seizes control of cellular cullin-RING E3 ubiquitin ligases (CRLs) to promote viral replication. HIV-1 Vpr and HIV-2/simian immunodeficiency virus (SIV) Vpr and Vpx engage the cullin4 (CUL4)-containing ubiquitin ligase complex (CRL4) to cause polyubiquitination and proteaso...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00241-14

    authors: Sharifi HJ,Furuya AK,Jellinger RM,Nekorchuk MD,de Noronha CM

    更新日期:2014-06-01 00:00:00

  • Murine gammaherpesvirus 68 open reading frame 24 is required for late gene expression after DNA replication.

    abstract::Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02726-06

    authors: Wong E,Wu TT,Reyes N,Deng H,Sun R

    更新日期:2007-06-01 00:00:00

  • Molecular profiling of ovine prion diseases by using thermolysin-resistant PrPSc and endogenous C2 PrP fragments.

    abstract::Disease-associated PrP fragments produced upon in vitro or in vivo proteolysis can provide significant insight into the causal strain of prion disease. Here we describe a novel molecular strain typing assay that used thermolysin digestion of caudal medulla samples to produce PrPres signatures on Western blots that rea...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00640-07

    authors: Owen JP,Rees HC,Maddison BC,Terry LA,Thorne L,Jackman R,Whitelam GC,Gough KC

    更新日期:2007-10-01 00:00:00

  • Avian reovirus nonstructural protein p17-induced G(2)/M cell cycle arrest and host cellular protein translation shutoff involve activation of p53-dependent pathways.

    abstract::The effects of avian reovirus (ARV) p17 protein on cell cycle progression and host cellular protein translation were studied. ARV infection and ARV p17 transfection resulted in the accumulation of infected and/or transfected cells in the G(2)/M phase of the cell cycle. The accumulation of cells in the G(2)/M phase was...

    journal_title:Journal of virology

    pub_type: 杂志文章,收录出版

    doi:10.1128/JVI.02604-09

    authors: Chulu JL,Huang WR,Wang L,Shih WL,Liu HJ

    更新日期:2010-08-01 00:00:00

  • Multiplication of parvovirus LuIII in a synchronized culture system. III. Replication of viral DNA.

    abstract::The replication of the single-stranded DNA (ssDNA) of parvovirus LuIII was studied in synchronized HeLa cells. After infection of the cells in early S phase, synthesis of a replicative form (RF) DNA became detectable as early as 9 h postinfection, i.e., after display of the cellular helper function(s) indispensable fo...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.17.3.841-853.1976

    authors: Siegl G,Gautschi M

    更新日期:1976-03-01 00:00:00

  • CD8+ T Cells Play a Bystander Role in Mice Latently Infected with Herpes Simplex Virus 1.

    abstract:UNLABELLED:Based on an explant reactivation model, it has been proposed that CD8(+) T cells maintain latency in trigeminal ganglia (TG) of mice latently infected with herpes simplex virus 1 (HSV-1) [T. Liu, K. M. Khanna, X. Chen, D. J. Fink, and R. L. Hendricks, J Exp Med 191:1459-1466, 2000, doi:10.1084/jem.191.9.1459...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00255-16

    authors: Mott KR,Gate D,Matundan HH,Ghiasi YN,Town T,Ghiasi H

    更新日期:2016-04-29 00:00:00

  • Attenuated Infectious Hematopoietic Necrosis Virus with Rearranged Gene Order as Potential Vaccine.

    abstract::The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective po...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01024-16

    authors: Rouxel RN,Tafalla C,Mérour E,Leal E,Biacchesi S,Brémont M

    更新日期:2016-11-14 00:00:00

  • Genetic and physiological studies of abortive infections of hydroxymethyluracil-containing bacteriophages in lysogens of temperate Bacillus subtilis bacteriophage SPO2.

    abstract::Wild-type bacteriophage phie and IS (interference-sensitive) mutants of the related phage SP82G did not productively infect strains of Bacillus subtilis that were lysogenic for temperate phage SPO2. In these abortive infections, the sensitive phages adsorbed to and penetrated the nonpermissive host, phage-directed mac...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.18.3.839-847.1976

    authors: Friedman SO,Hemphill HE

    更新日期:1976-06-01 00:00:00

  • Human parainfluenza virus type 3 HN-receptor interaction: effect of 4-guanidino-Neu5Ac2en on a neuraminidase-deficient variant.

    abstract::The envelope of human parainfluenza virus type 3 (HPF3) contains two viral glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion protein (F). HN, which is responsible for receptor attachment and for promoting F-mediated fusion, also possesses neuraminidase (receptor-destroying) activity. We reported previ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.16.7481-7488.2001

    authors: Porotto M,Greengard O,Poltoratskaia N,Horga MA,Moscona A

    更新日期:2001-08-01 00:00:00

  • Examination of the pRb-dependent and pRb-independent functions of E7 in vivo.

    abstract::High-risk human papillomaviruses encode two oncogenes, E6 and E7, expressed in nearly all cervical cancers. Although E7 protein is best known for its ability to inactivate the retinoblastoma tumor suppressor protein, pRb, many other activities for E7 have been proposed in in vitro studies. Herein, we describe studies ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.17.11392-11402.2005

    authors: Balsitis S,Dick F,Lee D,Farrell L,Hyde RK,Griep AE,Dyson N,Lambert PF

    更新日期:2005-09-01 00:00:00

  • Complete genome sequence of a Chinese virulent porcine epidemic diarrhea virus strain.

    abstract::CH/S is a virulent porcine epidemic diarrhea virus (PEDV) strain and is used as the virulent strain to evaluate the protection rates of vaccines against PEDV infection in China. Here, we report the complete genome sequence of strain CH/S, which may aid in understanding the molecular characteristics of this strain. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.06024-11

    authors: Chen J,Wang C,Shi H,Qiu HJ,Liu S,Shi D,Zhang X,Feng L

    更新日期:2011-11-01 00:00:00

  • Loss of the vaccinia virus 35-amino acid hydrophobic O3 protein is partially compensated by mutations in the transmembrane domains of other entry proteins.

    abstract::Eleven highly conserved proteins comprise the poxvirus entry-fusion complex (EFC). We focused on vaccinia virus (VACV) O3, a 35-amino acid, largely hydrophobic component of unknown specific function. Experimental evolution was carried out by blindly passaging a virus that was severely impaired in entry due to deletion...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02228-20

    authors: Tak AI,Americo JL,Diesterbeck US,Moss B

    更新日期:2021-01-27 00:00:00

  • Growth and survival of reovirus in intestinal tissue: role of the L2 and S1 genes.

    abstract::Reovirus serotype 1 Lang can be recovered in high titer from the intestines of neonatal mice up to day 8 after peroral inoculation. By contrast, reovirus serotype 3 Dearing cannot be recovered from intestinal tissue past day 4 after peroral inoculation. This difference between the two reoviruses was mapped by using re...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.3.1188-1193.1989

    authors: Bodkin DK,Fields BN

    更新日期:1989-03-01 00:00:00

  • Interactions of foot-and-mouth disease virus with soluble bovine alphaVbeta3 and alphaVbeta6 integrins.

    abstract::At least four members of the integrin family of receptors, alphaVbeta1, alphaVbeta3, alphaVbeta6, and alphaVbeta8, have been identified as receptors for foot-and-mouth disease virus (FMDV) in vitro. Our investigators have recently shown that the efficiency of receptor usage appears to be related to the viral serotype ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.78.18.9773-9781.2004

    authors: Duque H,LaRocco M,Golde WT,Baxt B

    更新日期:2004-09-01 00:00:00

  • Computational design of antiviral RNA interference strategies that resist human immunodeficiency virus escape.

    abstract::Recently developed antiviral strategies based upon RNA interference (RNAi), which harnesses an innate cellular system for the targeted down-regulation of gene expression, appear highly promising and offer alternative approaches to conventional highly active antiretroviral therapy or efforts to develop an AIDS vaccine....

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.3.1645-1654.2005

    authors: Leonard JN,Schaffer DV

    更新日期:2005-02-01 00:00:00

  • Activation of mitogen-activated protein kinase and NF-kappaB pathways by a Kaposi's sarcoma-associated herpesvirus K15 membrane protein.

    abstract::The K15 gene of Kaposi's sarcoma-associated herpesvirus (also known as human herpesvirus 8) consists of eight alternatively spliced exons and has been predicted to encode membrane proteins with a variable number of transmembrane regions and a common C-terminal cytoplasmic domain with putative binding sites for SH2 and...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.17.9346-9358.2003

    authors: Brinkmann MM,Glenn M,Rainbow L,Kieser A,Henke-Gendo C,Schulz TF

    更新日期:2003-09-01 00:00:00

  • Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses.

    abstract::Molecular clones of the subgroup A feline leukemia virus FeLV-A/Glasgow-1 have been obtained. Nucleotide sequence analysis of the 3' end of the proviral genome and comparison with the published sequence of FeLV-B/Gardner-Arnstein showed that the most extensive differences are located within the 5' domain of the env ge...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.58.3.825-834.1986

    authors: Stewart MA,Warnock M,Wheeler A,Wilkie N,Mullins JI,Onions DE,Neil JC

    更新日期:1986-06-01 00:00:00

  • Comparative ultrastructural study of four reticuloendothelias viruses.

    abstract::The morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. Virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain T are sperical with a diameter of approximately ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.16.4.1027-1038.1975

    authors: Kang CY,Wong TC,Holmes KV

    更新日期:1975-10-01 00:00:00

  • Assignment of the large oligonucleotides of vesicular stomatitis virus to the N, NS, M, G, and L genes and oligonucleotide gene ordering within the L gene.

    abstract::Analyses of prototype vesicular stomatitis (VSV, Indiana serotype) mRNA-32P-labeled viral RNA duplexes have established the assignments of 65 of the 72 large oligonucleotides that are recovered by two-dimensional electrophoresis of RNase T1 digests of the viral RNA. Fifty of the oligonucleotides are recovered in the L...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.30.1.116-123.1979

    authors: Clewley JP,Bishop DH

    更新日期:1979-04-01 00:00:00

  • Preemptive CD8 T-cell immunotherapy of acute cytomegalovirus infection prevents lethal disease, limits the burden of latent viral genomes, and reduces the risk of virus recurrence.

    abstract::In the immunocompetent host, primary cytomegalovirus (CMV) infection is resolved by the immune response without causing overt disease. The viral genome, however, is not cleared but is maintained in a latent state that entails a risk of virus recurrence and consequent organ disease. By using murine CMV as a model, we h...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.3.1797-1804.1998

    authors: Steffens HP,Kurz S,Holtappels R,Reddehase MJ

    更新日期:1998-03-01 00:00:00