Strandedness and complementarity of DNA from long-term RNA-dependent DNA polymerase reactions of Soehner-Dmochowski murine sarcoma virus.

Abstract:

:The DNA product of the endogenously instructed RNA-dependent DNA polymerase reaction of murine sarcoma virus continued to be synthesized for as long as 64 h in the presence of 0.008% Triton X-100. Higher detergent concentrations and actinomycin D inhibited DNA product synthesis. The DNA product from long-term polymerase reactions consisted of small DNA fragments as shown by sedimentation in alkaline sucrose gradients. The enzymatic DNA product was separated into a slow sedimenting fraction and a fast sedimenting fraction by rate-zonal centrifugation. Fast sedimenting DNA was the predominant fraction made in viral polymerase reactions containing 262 mM NaCl. By using a combination of S-1 nuclease and pancreatic RNase A, the amount of single-stranded DNA, double-stranded DNA, and DNA-RNA hybrid present in the slow-sedimenting and fast-sedimenting fractions was determined. Under standard polymerase conditions of 70 mM NaCl, single-stranded DNA was the major form of DNA found in both fractions. In contrast, the prevalent form of DNA made in the presence of 262 mM NaCl was DNA-RNA hybrid. Hybridization studies in which either S-1 nuclease or pancreatic RNase A was used to measure hybrid formation demonstrated not only that the DNA product was complementary in base sequence to the RNA genome, but also that at least 79 to 84% of the RNA genome was transcribed into complementary DNA.

journal_name

J Virol

journal_title

Journal of virology

authors

East JL,Knesek JE,Allen PT,Dmochowski L

doi

10.1128/JVI.12.5.1049-1064.1973

subject

Has Abstract

pub_date

1973-11-01 00:00:00

pages

1049-64

issue

5

eissn

0022-538X

issn

1098-5514

journal_volume

12

pub_type

杂志文章
  • Origin of the vaccinia virus hemagglutinin.

    abstract::The relationship between the vaccinia virus hemagglutinin and hemadsorption was examined. Hemagglutinin synthesis was temporally related to the appearance of the hemadsorption reaction. Only chicken erythrocytes, which reacted with hemagglutinin, hemadsorbed to infected cells, and both of these reactions were inhibite...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.9.2.290-296.1972

    authors: Blackman KE,Bubel HC

    更新日期:1972-02-01 00:00:00

  • Heterotypic humoral and cellular immune responses following Norwalk virus infection.

    abstract::Norovirus immunity is poorly understood as the limited data available on protection after infection are often contradictory. In contrast to the more prominent GII noroviruses, GI norovirus infections are less frequent in outbreaks. The GI noroviruses display very complex patterns of heterotypic immune responses follow...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02179-09

    authors: Lindesmith LC,Donaldson E,Leon J,Moe CL,Frelinger JA,Johnston RE,Weber DJ,Baric RS

    更新日期:2010-02-01 00:00:00

  • The A20R protein is a stoichiometric component of the processive form of vaccinia virus DNA polymerase.

    abstract::In vitro analysis of the catalytic DNA polymerase encoded by vaccinia virus has demonstrated that it is innately distributive, catalyzing the addition of <10 nucleotides per primer-template binding event in the presence of 8 mM MgCl(2) or 40 mM NaCl (W. F. McDonald and P. Traktman, J. Biol. Chem. 269:31190-31197, 1994...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.24.12298-12307.2001

    authors: Klemperer N,McDonald W,Boyle K,Unger B,Traktman P

    更新日期:2001-12-01 00:00:00

  • Persistent infection of a rat nephroma cell line with kilham rat virus.

    abstract::A rat nephroma cell line was found to be a carrier culture of the Kilham rat virus. Infectious virus was demonstrable only by passage of culture materials into other host systems. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.4.3.313-314.1969

    authors: Wozniak J,Hetrick F

    更新日期:1969-09-01 00:00:00

  • Phosphorylation of murine mammary tumor virus precursor polypeptides.

    abstract::Phosphorylation of the murine mammary tumor virus (MuMTV) structural proteins was studied in an MuMTV-infected epithelial cell line derived from a BALB/cf C3H mouse mammary tumor. Immunoprecipitation of 32P-labeled cell extracts with monospecific anti-p27 serum revealed that phosphorylation occurred at the stage of th...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.30.1.241-247.1979

    authors: Racevskis J,Sarkar NH

    更新日期:1979-04-01 00:00:00

  • Murine AIDS is initiated in the lymph nodes draining the site of inoculation, and the infected B cells influence T cells located at distance, in noninfected organs.

    abstract::The infection of cells which belong to the B-cell lineage is thought to be the primary event leading to the phenotypic and functional alterations seen in the murine AIDS (M. Huang, C. Simard, D. Kay, and P. Jolicoeur, J. Virol. 65:6562-6571, 1991). Using in situ hybridization, we studied the time course of the anatomi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.3.1903-1912.1994

    authors: Simard C,Huang M,Jolicoeur P

    更新日期:1994-03-01 00:00:00

  • Morphology of a virus of blue-green algae and properties of its deoxyribonucleic acid.

    abstract::The morphology of Safferman's virus of blue-green algae (phycovirus LPP-1) has been studied by electron microscopy and physicochemical methods. The virion has a short (100 to 200 A long, 150 A in diameter) forked tail, with an outer sheath, an inner core, and a capital attached to one of the vertices of a polyhedral h...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.1.2.344-361.1967

    authors: Luftig R,Haselkorn R

    更新日期:1967-04-01 00:00:00

  • Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus.

    abstract::A contiguously linked T-B synthetic viral epitope (110HA120-150HA159,T-B) was investigated for its potency in inducing humoral and cellular immune responses in vivo. The T-cell epitope 110HA120 corresponds to the site 1 hemagglutinin (HA) of the A/PR/8/34 (PR8) influenza virus and is recognized by CD4 T cells in assoc...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.7.5473-5480.1997

    authors: Brumeanu TD,Casares S,Bot A,Bot S,Bona CA

    更新日期:1997-07-01 00:00:00

  • Contribution of glutamine residues in the helix 4-5 loop to capsid-capsid interactions in simian immunodeficiency virus of macaques.

    abstract:UNLABELLED:Following retrovirus entry, the viral capsid (CA) disassembles into its component capsid proteins. The rate of this uncoating process, which is regulated by CA-CA interactions and by the association of the capsid with host cell factors like cyclophilin A (CypA), can influence the efficiency of reverse transc...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01388-14

    authors: Tipper C,Sodroski JG

    更新日期:2014-09-01 00:00:00

  • CD1d mediates T-cell-dependent resistance to secondary infection with encephalomyocarditis virus (EMCV) in vitro and immune response to EMCV infection in vivo.

    abstract::The innate and adaptive immune responses have evolved distinct strategies for controlling different viral pathogens. Encephalomyocarditis virus (EMCV) is a picornavirus that can cause paralysis, diabetes, and myocarditis within days of infection. The optimal innate immune response against EMCV in vivo requires CD1d. I...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02745-05

    authors: Ilyinskii PO,Wang R,Balk SP,Exley MA

    更新日期:2006-07-01 00:00:00

  • Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2.

    abstract::Polymerase basic protein 2 (PB2), a component of the influenza virus polymerase complex, when expressed alone from cloned cDNA in the absence of other influenza virus proteins, is transported into the nucleus. In this study, we have examined the nuclear translocation signal of PB2 by making deletions and mutations in ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.1.245-253.1991

    authors: Mukaigawa J,Nayak DP

    更新日期:1991-01-01 00:00:00

  • Mapping of functional domains of the lipid kinase phosphatidylinositol 4-kinase type III alpha involved in enzymatic activity and hepatitis C virus replication.

    abstract:UNLABELLED:The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). PI4KIIIα is an essential host factor for hepatitis C virus (HCV) replication. Interaction with HCV nonstructural protein 5A (NS5A) lea...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01063-14

    authors: Harak C,Radujkovic D,Taveneau C,Reiss S,Klein R,Bressanelli S,Lohmann V

    更新日期:2014-09-01 00:00:00

  • Addition of extra DNA sequences to simian virus 40 DNA in vivo.

    abstract::The possible addition of extra sequences to simian virus 40 (SV40) DNA was analyzed by electron microscopy in two different cell systems, productively infected monkey cells and activated heterokaryons on monkey and transformed mouse 3T3 cells. We found that the closed circular DNA fraction, extracted from monkey cells...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.23.2.323-337.1977

    authors: Yoshike K,Defendi V

    更新日期:1977-08-01 00:00:00

  • Poliovirus/Hepatitis C virus (internal ribosomal entry site-core) chimeric viruses: improved growth properties through modification of a proteolytic cleavage site and requirement for core RNA sequences but not for core-related polypeptides.

    abstract::H.-H. Lu and E. Wimmer (Proc. Natl. Acad. Sci. USA 93:1412-1417, 1996) have demonstrated that the internal ribosomal entry site (IRES) of poliovirus (PV) can be functionally replaced by the related genetic element from hepatitis C virus (HCV). One important finding of this study was that open reading frame sequences 3...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.2.1546-1554.1999

    authors: Zhao WD,Wimmer E,Lahser FC

    更新日期:1999-02-01 00:00:00

  • G2/M cell cycle arrest correlates with primate lentiviral Vpr interaction with the SLX4 complex.

    abstract:UNLABELLED:The accessory gene vpr, common to all primate lentiviruses, induces potent G2/M arrest in cycling cells. A recent study showed that human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi anem...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02307-14

    authors: Berger G,Lawrence M,Hué S,Neil SJ

    更新日期:2015-01-01 00:00:00

  • The genomic RNA in Ty1 virus-like particles is dimeric.

    abstract::The yeast retrotransposon Ty1 resembles retroviruses in a number of important respects but also shows several fundamental differences from them. We now report that, as in retroviruses, the genomic RNA in Ty1 virus-like particles is dimeric. The Ty1 dimers also resemble retroviral dimers in that they are stabilized dur...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.22.10819-10821.2000

    authors: Feng YX,Moore SP,Garfinkel DJ,Rein A

    更新日期:2000-11-01 00:00:00

  • Multiple Introductions and Antigenic Mismatch with Vaccines May Contribute to Increased Predominance of G12P[8] Rotaviruses in the United States.

    abstract::Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01476-18

    authors: Ogden KM,Tan Y,Akopov A,Stewart LS,McHenry R,Fonnesbeck CJ,Piya B,Carter MH,Fedorova NB,Halpin RA,Shilts MH,Edwards KM,Payne DC,Esona MD,Mijatovic-Rustempasic S,Chappell JD,Patton JT,Halasa NB,Das SR

    更新日期:2018-12-10 00:00:00

  • Intrauterine transmission of human T-cell leukemia virus type I in rats.

    abstract::To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.2.1302-1305.1995

    authors: Hori M,Ami Y,Kushida S,Kobayashi M,Uchida K,Abe T,Miwa M

    更新日期:1995-02-01 00:00:00

  • Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus.

    abstract::Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.13.1.42-45.1974

    authors: Fritz RB

    更新日期:1974-01-01 00:00:00

  • Potent vesicular stomatitis virus-based avian influenza vaccines provide long-term sterilizing immunity against heterologous challenge.

    abstract::The emergence in 1997 and continuance today of a highly lethal H5N1 avian influenza virus (AIV) causing human disease has raised concern about an impending pandemic and the need for a vaccine to prepare for such an occurrence. We previously generated an efficacious vesicular stomatitis virus (VSV)-based AIV vaccine ex...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02637-09

    authors: Schwartz JA,Buonocore L,Suguitan AL Jr,Silaghi A,Kobasa D,Kobinger G,Feldmann H,Subbarao K,Rose JK

    更新日期:2010-05-01 00:00:00

  • Immunological relationships of an endogenous guinea pig retrovirus with prototype mammalian type B and type D retroviruses.

    abstract::A retrovirus endogenous to guinea pig cells was earlier shown to be morphologically similar to type B and type D prototype retroviruses. Molecular hybridization techniques were used to show that guinea pig virus nucleotide sequences are endogenous to both domestic (Cavia porcellus) and indigenous (Cavia aperea) guinea...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.33.1.522-530.1980

    authors: Dahlberg JE,Tronick SR,Aaronson SA

    更新日期:1980-01-01 00:00:00

  • Cooperation between Strain-Specific and Broadly Neutralizing Responses Limited Viral Escape and Prolonged the Exposure of the Broadly Neutralizing Epitope.

    abstract::V3-glycan-targeting broadly neutralizing antibodies (bNAbs) are a focus of HIV-1 vaccine development. Understanding the viral dynamics that stimulate the development of these antibodies can provide insights for immunogen design. We used a deep-sequencing approach, together with neutralization phenotyping, to investiga...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00828-17

    authors: Anthony C,York T,Bekker V,Matten D,Selhorst P,Ferreria RC,Garrett NJ,Karim SSA,Morris L,Wood NT,Moore PL,Williamson C

    更新日期:2017-08-24 00:00:00

  • Evolution of the equine infectious anemia virus long terminal repeat during the alteration of cell tropism.

    abstract::Equine infectious anemia virus (EIAV) is a lentivirus with in vivo cell tropism primarily for tissue macrophages; however, in vitro the virus can be adapted to fibroblasts and other cell types. Tropism adaptation is associated with both envelope and long terminal repeat (LTR) changes, and findings strongly suggest tha...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.9.5653-5664.2005

    authors: Maury W,Thompson RJ,Jones Q,Bradley S,Denke T,Baccam P,Smazik M,Oaks JL

    更新日期:2005-05-01 00:00:00

  • Precise identification of a human immunodeficiency virus type 1 antigen processing mutant.

    abstract::Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus persists. Polymorphisms within known antigenic sites result in loss of immune recognition and can be positively selected. Amino acid variation outside known HLA class I restricted epitopes can also enable immune escape by interf...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00968-06

    authors: Zimbwa P,Milicic A,Frater J,Scriba TJ,Willis A,Goulder PJ,Pillay T,Gunthard H,Weber JN,Zhang HT,Phillips RE

    更新日期:2007-02-01 00:00:00

  • Integrated Moloney murine leukemia virus DNA studied by using complementary DNA which does not recognize endogenous related sequences.

    abstract::By preannealing a radioactive, representative Moloney murine leukemia virus (M-MuLV) cDNA with large excesses of AKR 70S viral RNA, an M-MuLV-specific cDNA has been prepared. When hybridized to restriction enzyme fragments of M-MuLV-infected mouse cell DNA, the preannealed probe recognizes integrated M-MuLV DNA and do...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.33.3.1074-1082.1980

    authors: Bacheler LT,Fan H

    更新日期:1980-03-01 00:00:00

  • Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle.

    abstract::Novel molecular approaches to inhibit human immunodeficiency virus type 1 (HIV-1) infection have received increasing attention because of the lack of effective antiviral drug therapies in vivo. We now demonstrate that cells can be intracellularly immunized by cytoplasmic expression of single-chain variable antibody fr...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.6.3392-3400.1996

    authors: Shaheen F,Duan L,Zhu M,Bagasra O,Pomerantz RJ

    更新日期:1996-06-01 00:00:00

  • Attenuated Infectious Hematopoietic Necrosis Virus with Rearranged Gene Order as Potential Vaccine.

    abstract::The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective po...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01024-16

    authors: Rouxel RN,Tafalla C,Mérour E,Leal E,Biacchesi S,Brémont M

    更新日期:2016-11-14 00:00:00

  • Extracellular vaccinia virus envelope glycoprotein encoded by the A33R gene.

    abstract::With the aid of three monoclonal antibodies (MAbs), a glycoprotein specifically localized to the outer envelope of vaccinia virus was shown to be encoded by the A33R gene. These MAbs reacted with a glycosylated protein that migrated as 23- to 28-kDa and 55-kDa species under reducing and nonreducing conditions, respect...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.6.3753-3762.1996

    authors: Roper RL,Payne LG,Moss B

    更新日期:1996-06-01 00:00:00

  • Mutational analysis of the cleavage sequence of the human immunodeficiency virus type 1 envelope glycoprotein precursor gp160.

    abstract::The envelope glycoproteins of the human immunodeficiency virus (HIV) type 1 are synthesized as a precursor molecule, gp160, which is cleaved to generate the two mature envelope glycoproteins, gp120 and gp41. The cleavage reaction, which is mediated by a host protease, occurs at a sequence highly conserved in retrovira...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.11.4670-4675.1989

    authors: Freed EO,Myers DJ,Risser R

    更新日期:1989-11-01 00:00:00

  • Nuclear export of human immunodeficiency virus type 1 Vpr is not required for virion packaging.

    abstract::The human immunodeficiency virus type 1 Vpr protein is both packaged into virions and efficiently localized to the nucleus. In this report, we show that a significant fraction of Vpr also accumulates in the cytoplasm of virus-producing cells. Although Vpr shuttles between the nucleus and the cytoplasm, studies with an...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.75.17.8348-8352.2001

    authors: Jenkins Y,Sanchez PV,Meyer BE,Malim MH

    更新日期:2001-09-01 00:00:00