Abstract:
:Rotavirus is the leading global cause of diarrheal mortality for unvaccinated children under 5 years of age. The outer capsid of rotavirus virions consists of VP7 and VP4 proteins, which determine viral G and P types, respectively, and are primary targets of neutralizing antibodies. Successful vaccination depends upon generating broadly protective immune responses following exposure to rotaviruses presenting a limited number of G- and P-type antigens. Vaccine introduction resulted in decreased rotavirus disease burden but also coincided with the emergence of uncommon G and P genotypes, including G12. To gain insight into the recent predominance of G12P[8] rotaviruses in the United States, we evaluated 142 complete rotavirus genome sequences and metadata from 151 clinical specimens collected in Nashville, TN, from 2011 to 2013 through the New Vaccine Surveillance Network. Circulating G12P[8] strains were found to share many segments with other locally circulating strains but to have distinct constellations. Phylogenetic analyses of G12 sequences and their geographic sources provided evidence for multiple separate introductions of G12 segments into Nashville, TN. Antigenic epitopes of VP7 proteins of G12P[8] strains circulating in Nashville, TN, differ markedly from those of vaccine strains. Fully vaccinated children were found to be infected with G12P[8] strains more frequently than with other rotavirus genotypes. Multiple introductions and significant antigenic mismatch may in part explain the recent predominance of G12P[8] strains in the United States and emphasize the need for continued monitoring of rotavirus vaccine efficacy against emerging rotavirus genotypes.IMPORTANCE Rotavirus is an important cause of childhood diarrheal disease worldwide. Two immunodominant proteins of rotavirus, VP7 and VP4, determine G and P genotypes, respectively. Recently, G12P[8] rotaviruses have become increasingly predominant. By analyzing rotavirus genome sequences from stool specimens obtained in Nashville, TN, from 2011 to 2013 and globally circulating rotaviruses, we found evidence of multiple introductions of G12 genes into the area. Based on sequence polymorphisms, VP7 proteins of these viruses are predicted to present themselves to the immune system very differently than those of vaccine strains. Many of the sick children with G12P[8] rotavirus in their diarrheal stools also were fully vaccinated. Our findings emphasize the need for continued monitoring of circulating rotaviruses and the effectiveness of the vaccines against strains with emerging G and P genotypes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ogden KM,Tan Y,Akopov A,Stewart LS,McHenry R,Fonnesbeck CJ,Piya B,Carter MH,Fedorova NB,Halpin RA,Shilts MH,Edwards KM,Payne DC,Esona MD,Mijatovic-Rustempasic S,Chappell JD,Patton JT,Halasa NB,Das SRdoi
10.1128/JVI.01476-18subject
Has Abstractpub_date
2018-12-10 00:00:00issue
1eissn
0022-538Xissn
1098-5514pii
JVI.01476-18journal_volume
93pub_type
杂志文章abstract::Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02586-07
更新日期:2008-05-01 00:00:00
abstract::Murine cytomegalovirus encodes three regulators of antigen presentation to antiviral CD8 T cells. According to current paradigms, all three regulators are committed to the inhibition of the presentation of antigenic peptides. Whereas m152/gp40 catalyzes the retention of peptide-loaded major histocompatibility complex ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00723-06
更新日期:2006-08-01 00:00:00
abstract::Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.2.503-510.1982
更新日期:1982-08-01 00:00:00
abstract::We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.625-634.1981
更新日期:1981-12-01 00:00:00
abstract::West Nile virus (WNV), currently the cause of a serious U.S. epidemic, is a mosquito-borne flavivirus and member of the Japanese encephalitis (JE) serocomplex. There is currently no approved human WNV vaccine, and treatment options remain limited, resulting in significant mortality and morbidity from human infection. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00480-13
更新日期:2013-09-01 00:00:00
abstract::The hepatitis B virus (HBV) capsid or core protein (Cp) can self-assemble to form an icosahedral capsid. It is now being pursued as a target for small-molecule antivirals that enhance the rate and extent of its assembly to yield empty and/or aberrant capsids. These small molecules are thus called core protein alloster...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01082-18
更新日期:2018-09-26 00:00:00
abstract::Hepatitis C virus (HCV) reorganizes intracellular membranes to establish sites of replication. How viral and cellular proteins target, bind, and rearrange specific membranes into the replication factory remains a mystery. We used a lentivirus-based RNA interference (RNAi) screening approach to identify the potential c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06001-11
更新日期:2012-02-01 00:00:00
abstract::Merkel cell carcinoma (MCC) is the most aggressive skin cancer. Recently, it was demonstrated that human Merkel cell polyomavirus (MCV) is clonally integrated in approximately 80% of MCC tumors. However, direct evidence for whether oncogenic viral proteins are needed for the maintenance of MCC cells is still missing. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02400-09
更新日期:2010-07-01 00:00:00
abstract::Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.299-305.1978
更新日期:1978-05-01 00:00:00
abstract::To clarify hematological abnormalities following infection with human immunodeficiency virus (HIV), we examined the hematopoietic capability of bone marrow by using cynomolgus monkeys infected with pathogenic simian/human immunodeficiency virus (SHIV) strain C2/1, an animal model of HIV infection. The relationship bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.10906-10910.2004
更新日期:2004-10-01 00:00:00
abstract::Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas worldwide. An improved understanding of the regions within the DENV genome and its encoded proteins that are required for the virus replication cycle will expedite the development of urgentl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01455-17
更新日期:2017-11-14 00:00:00
abstract::We tested whether the selection of target sites can be manipulated by fusing retroviral integrase with a sequence-specific DNA-binding protein. A hybrid protein that has the Escherichia coli LexA protein fused to the C terminus of the human immunodeficiency virus type 1 integrase was constructed. The fusion protein, I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.37-46.1996
更新日期:1996-01-01 00:00:00
abstract::A recent publication indicated that overexpression of Axl, a cellular receptor that negatively regulates Toll-like receptor signaling, enhanced the entry of viruses pseudotyped with the glycoprotein of lymphocytic choriomeningitis virus (LCMV) in vitro. In testing the biological relevance of these observations, we fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
abstract::BK polyomavirus (BKV) establishes persistent, low-level, and asymptomatic infections in most humans and causes polyomavirus-associated nephropathy (PVAN) and other pathologies in some individuals. The activation of BKV replication following kidney transplantation, leading to viruria, viremia, and, ultimately, PVAN, is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06369-11
更新日期:2012-03-01 00:00:00
abstract::We have previously designed in vitro model systems to characterize the herpes simplex virus type 1 (HSV-1) genome during in vitro virus latency. Latency was established by treatment of infected human embryo lung fibroblast (HEL-F) cells or rat fetal neurons with (E)-5-(2-bromovinyl)-2'-deoxyuridine and human leukocyte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.205-213.1984
更新日期:1984-01-01 00:00:00
abstract::We have developed a system in which vesicular stomatitis virus (VSV) minigenomes encoding viral structural proteins can be expressed from plasmids. These RNAs can be replicated, transcribed, and packaged into infectious particles when coexpressed with the other VSV proteins. The minigenomes contain either the glycopro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2946-2953.1995
更新日期:1995-05-01 00:00:00
abstract::The herpes simplex virus type 1 UL6 protein forms a 12-subunit ring structure at a unique capsid vertex which functions as a conduit for encapsidation of the viral genome. To characterize UL6 protein domains that are involved in intersubunit interactions and interactions with other capsid proteins, we engineered a set...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00739-07
更新日期:2007-09-01 00:00:00
abstract::Group I host range (hr) mutants of adenovirus type 5 are unable to transform rat embryo or rat embryo brain cells but induce an abnormal transformation of baby rat kidney cells. We established several transformed rat kidney cell lines and characterized them with respect to the transformed phenotype and the structure o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.674-685.1982
更新日期:1982-02-01 00:00:00
abstract:UNLABELLED:Viral myocarditis, which is most prevalently caused by coxsackievirus B3 (CVB3), is a serious clinical condition characterized by excessive myocardial inflammation. Recent studies suggest that regulation of protein acetylation levels by inhibiting histone deacetylase (HDAC) activity modulates inflammatory re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01028-15
更新日期:2015-10-01 00:00:00
abstract::The Duplan strain of murine leukemia virus induces murine AIDS in C57BL/6 mice. When spleen cells from C57BL/6 mice infected with the virus were transplanted into nude mice, subcutaneous solid tumors at the transplanted sites were formed and splenomegaly and lymphadenopathy were induced. These transplantable cells wer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5691-5695.1992
更新日期:1992-09-01 00:00:00
abstract::Two types of geminate structures were purified from African cassava mosaic geminivirus (ACMV)-infected Nicotiana benthamiana plants and analyzed by electron cryomicroscopy and image reconstruction. After cesium sulfate density gradient centrifugation, they were separated into lighter top (T) and heavier bottom (B) com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.6758-6765.2004
更新日期:2004-07-01 00:00:00
abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.625-631.1973
更新日期:1973-09-01 00:00:00
abstract::We analyzed a region of the capsid of canine parvovirus (CPV) which determines the ability of the virus to infect canine cells. This region is distinct from those previously shown to determine the canine host range differences between CPV and feline panleukopenia virus. It lies on a ridge of the threefold spike of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9214-9222.1997
更新日期:1997-12-01 00:00:00
abstract::Several groups have reported that certain herpesvirus envelope proteins do not remain on the surface of cells that express them but rather are internalized by endocytosis in a recycling process. The biological function of membrane protein endocytosis in the virus life cycle remains a matter of speculation and debate. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4571-4579.1998
更新日期:1998-06-01 00:00:00
abstract::The HIV-1 spike is composed of three protomeric units, each containing a peripheral gp120 and a transmembrane gp41 subunit. Binding to the CD4 and the chemokine receptors triggers them to mediate virus entry into cells by membrane fusion. The spikes also represent the major target for neutralizing antibodies (Abs) aga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00530-13
更新日期:2013-06-01 00:00:00
abstract::We report here that sex hormones modulate susceptibility to a sexually transmitted viral agent, herpes simplex virus type 2 (HSV-2), in a mouse model. Ovariectomized mice were administered either saline (control), estradiol (E(2)), progesterone (P(4)), or a combination of both estradiol and progesterone (E+P) and infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.3107-3116.2005
更新日期:2005-03-01 00:00:00
abstract::Defective interfering particles (DIPs) of equine herpesvirus 1 (EHV-1; Kentucky A strain) mediate persistent infection. DNA sequences at the L terminus, which contain the UL2 gene (homolog of UL55 of herpes simplex virus type 1 and open reading frame 3 of varicella-zoster virus) of standard EHV-1, have been shown to b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2255-2265.1993
更新日期:1993-04-01 00:00:00
abstract::In Escherichia coli B infected with phage T4 with mutations in gene 55 the shut-off of synthesis of two early enzymes was found to be delayed 10 to 15 min as compared to wild-type T4 infection. The kinetics of early enzyme formation was very similar to that obtained after DNA-negative mutant infection. The transcripti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.1.49-57.1973
更新日期:1973-07-01 00:00:00
abstract::Complementary DNA clones encoding the murine homolog (mCAR) of the human coxsackievirus and adenovirus receptor (CAR) were isolated. Nonpermissive CHO cells transfected with mCAR cDNA became susceptible to infection by coxsackieviruses B3 and B4 and showed increased susceptibility to adenovirus-mediated gene transfer....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.415-419.1998
更新日期:1998-01-01 00:00:00
abstract::Epstein-Barr virus (EBV) in vivo is known to establish persistent infection in resting, circulating memory B cells and to productively replicate in plasma cells. Until now, the molecular mechanism of how EBV switches from latency to lytic replication in vivo was not known. Here, we report that the plasma cell differen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01055-07
更新日期:2007-12-01 00:00:00