Abstract:
:Cloned avian leukosis viral DNAs were mutagenized in the long terminal repeat, in the leader sequence for env mRNA, and at the poly-env junction. The effect of these mutations in the viral DNA upon its ability to direct virus production or env mRNA synthesis was analyzed by microinjecting the mutant DNAs into chicken embryo fibroblasts and into chicken cells transformed by the env-deficient Bryan strain of Rous sarcoma virus, respectively. The results indicated that: (i) addition of up to 8 base pairs 19 nucleotides upstream of the Hogness box did not block transcription; (ii) deletion of 26 base pairs, including the tRNA primer binding site, allowed synthesis of all viral products and participation in recombination, but replication was blocked; (iii) deletion of fewer than 50 base pairs 250 bases downstream of the long terminal repeat depressed expression of all viral genes; and (iv) deletion of most of the gag and pol genes did not inhibit env mRNA synthesis, but virion packaging of the unspliced transcript was inefficient.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pugatsch T,Stacey DWdoi
10.1128/JVI.43.2.503-510.1982subject
Has Abstractpub_date
1982-08-01 00:00:00pages
503-10issue
2eissn
0022-538Xissn
1098-5514journal_volume
43pub_type
杂志文章abstract::The Kansas strain of bovine parainfluenza virus type 3 (BPIV3) is 100- to 1,000-fold restricted in replication in the respiratory tracts of nonhuman primates compared to human PIV3 (HPIV3), an important pathogen of infants and young children. BPIV3 is also restricted in replication in human infants and children, yet i...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.47-53.1988
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1988-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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doi:10.1128/JVI.01793-17
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pub_type: 杂志文章
doi:10.1128/JVI.01767-12
更新日期:2012-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-09
更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6315-6324.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02369-12
更新日期:2013-02-01 00:00:00
abstract::The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1195-1202.1993
更新日期:1993-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02039-07
更新日期:2008-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00653-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2056-2062.2003
更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01636-06
更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00039-17
更新日期:2017-03-29 00:00:00
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pub_type: 杂志文章
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更新日期:1970-07-01 00:00:00
abstract::Picornavirus genomes encode unique 5' noncoding regions (5' NCRs) which are approximately 600 to 1,300 nucleotides in length, contain multiple upstream AUG codons, and display the ability to form extensive secondary structures. A number of recent reports have shown that picornavirus 5' NCRs are able to facilitate cap-...
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pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.172-182.1992
更新日期:1992-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.6900-6909.1994
更新日期:1994-11-01 00:00:00
abstract::The question of whether any mammalian cells are able to mount an effective RNA interference-mediated antiviral innate immune response has remained highly controversial. In this Gem, I review recent data addressing this important issue and propose a testable hypothesis that can explain many of the apparently contradict...
journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2014-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2667-2673.1989
更新日期:1989-06-01 00:00:00
abstract::Pigs are permissive to both human and avian influenza viruses and have been proposed to be an intermediate host for the genesis of pandemic influenza viruses through reassortment or adaptation of avian viruses. Prospective virological surveillance carried out between March 1998 and June 2000 in Hong Kong, Special Admi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9679-9686.2001
更新日期:2001-10-01 00:00:00
abstract::RNA silencing is a potent means of antiviral defense in plants and animals. A hallmark of this defense response is the production of 21- to 24-nucleotide viral small RNAs via mechanisms that remain to be fully understood. Many viruses encode suppressors of RNA silencing, and some viral RNAs function directly as silenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02339-06
更新日期:2007-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12608-12613.2005
更新日期:2005-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8868-8874.1997
更新日期:1997-11-01 00:00:00
abstract::Cytomegalovirus (CMV) is acquired by the oral route in children, and primary infection is associated with abundant mucosal replication, as well as the establishment of latency in myeloid cells that results in lifelong infection. The efficiency of primary CMV infection in humans following oral exposure, however, is unk...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00380-17
更新日期:2017-05-26 00:00:00