Abstract:
:We used virus assay and in situ hybridization with a cloned fragment of the murine cytomegalovirus (MCMV) genome to study MCMV infection of circulating leukocytes harvested from 3-week-old BALB/c, C57BL/6, and C3H mice infected with MCMV intraperitoneally. Infectious virus or MCMV DNA was detected in leukocytes on days 1 through 21 of infection in BALB/c mice and on days 3 through 7 in C57BL/6 mice. On days 5 and 7, MCMV DNA or infectious virus was detected in the leukocytes of 17 (94%) of 18 BALB/c mice and 10 (59%) of 17 C57BL/6 mice. In both strains infection peaked on days 5 and 7, when as many as 0.01 to 0.1% of the circulating leukocytes contained MCMV DNA. In C3H mice, however, infectious virus was rarely recovered from leukocyte fractions and MCMV DNA was detected in the circulating leukocytes of only one animal. Circulating leukocytes may have an important role in the dissemination of CMV infections in susceptible hosts.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bale JF Jr,O'Neil MEdoi
10.1128/JVI.63.6.2667-2673.1989subject
Has Abstractpub_date
1989-06-01 00:00:00pages
2667-73issue
6eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
杂志文章abstract:UNLABELLED:Cavally virus (CavV) and related viruses in the family Mesoniviridae diverged profoundly from other nidovirus lineages but largely retained the characteristic set of replicative enzymes conserved in the Coronaviridae and Roniviridae. The expression of these enzymes in virus-infected cells requires the extens...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02040-14
更新日期:2014-12-01 00:00:00
abstract::The roles of the capsid protein (CP) and the CP coding sequence of tobacco etch potyvirus (TEV) in genome amplification were analyzed. A series of frameshift-stop codon mutations that interrupted translation of the CP coding sequence at various positions were introduced into the TEV genome. A series of 3' deletion mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4370-4379.1996
更新日期:1996-07-01 00:00:00
abstract::Influenza viruses often evade host immunity via antigenic drift and shift despite previous influenza virus infection and/or vaccination. Vaccines that match circulating virus strains are needed for optimal protection. Development of a universal influenza virus vaccine providing broadly cross-protective immunity will b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00120-13
更新日期:2013-05-01 00:00:00
abstract::Macrophage adherence, an important regulatory signal, has the potential to affect human immunodeficiency virus (HIV) production either directly or by priming monocytes to respond to other activating signals. We have investigated the role of adherence as an activator of HIV-1 transcription and release. The effects of a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3569-3575.1993
更新日期:1993-06-01 00:00:00
abstract::RNA interference (RNAi) is a conserved antiviral immune defense in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to counteract antiviral RNAi. Alphaviruses are a large group of positive-stranded RNA viruses that maintain their transmission and life cycles in both mosquitoe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01233-19
更新日期:2020-01-17 00:00:00
abstract::It has been shown previously in the severe acute respiratory syndrome coronavirus (SARS-CoV) that two point mutations, N15A and V25F, in the transmembrane domain (TMD) of the envelope (E) protein abolished channel activity and led to in vivo attenuation. Pathogenicity was recovered in mutants that also regained E prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02158-16
更新日期:2017-02-14 00:00:00
abstract::The genome of the defective interfering (DI) mouse hepatitis virus DI-a carries a large open reading frame (ORF) consisting of ORF1a, ORF1b, and nucleocapsid sequences. To test whether this fusion ORF is important for DI virus replication, we constructed derivatives of the DI-a genome in which the reading frame was tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5898-5905.1992
更新日期:1992-10-01 00:00:00
abstract::We previously reported that human cytomegalovirus (HCMV) infection induces endoplasmic reticulum (ER) stress, resulting in activation of the unfolded protein response (UPR). Although some normal consequences of UPR activation (e.g., translation attenuation) are detrimental to viral infection, we have previously shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15388-15397.2005
更新日期:2005-12-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection results in the disruption of ND10 (also called nuclear bodies, PODs, or PML-associated bodies), which are nuclear matrix domains of unknown function present in mammalian cells. After ND10 disruption, viral transcription and DNA replication occur in globular nuclear domains...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10100-10107.1998
更新日期:1998-12-01 00:00:00
abstract::Bats are the second largest group of mammals on earth and act as reservoirs of many emerging viruses. In this study, a novel bat adenovirus (AdV) (BtAdV-TJM) was isolated from bat fecal samples by using a bat primary kidney cell line. Infection studies indicated that most animal and human cell lines are susceptible to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02497-09
更新日期:2010-04-01 00:00:00
abstract::The herpes simplex virus type 1 latency-associated transcript (LAT) inhibits apoptosis. We demonstrate here that LAT influences the accumulation of the Bcl-x(L) transcript versus the Bcl-x(S) transcript in Neuro-2A cells. Bcl-x(L) encodes an antiapoptotic protein, whereas Bcl-x(S) encodes a proapoptotic protein. Promo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10714-10718.2003
更新日期:2003-10-01 00:00:00
abstract::Aluminum (Al)-based salts are widely used adjuvants in ruminants and other species to strengthen the immune response elicited against vaccine antigen(s). However, they can lead to the formation of long-lasting granulomas composed of abundant activated macrophages. Small ruminant lentiviruses (SRLV) are widely distribu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-20
更新日期:2020-12-22 00:00:00
abstract::Polyamides have been shown to bind double-stranded DNA by complementing the curvature of the minor groove and forming various hydrogen bonds with DNA. Several polyamide molecules have been found to have potent antiviral activities against papillomavirus, a double-stranded DNA virus. By analogy, we reason that polyamid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00146-18
更新日期:2018-03-28 00:00:00
abstract::The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.534-539.1975
更新日期:1975-03-01 00:00:00
abstract::West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent or treat disease. Passive immunization with antibodies against the envelope protein represents a promising means to provide short-term prophylaxis and treatment for West Nile virus infect...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14606-14613.2005
更新日期:2005-12-01 00:00:00
abstract::Murine cytomegalovirus (MCMV) proteins m142 and m143 are essential for viral replication. They bind double-stranded RNA and prevent protein kinase R-induced protein synthesis shutoff. Whether the two viral proteins have additional functions such as their homologs in human cytomegalovirus do remained unknown. We show t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02003-15
更新日期:2015-10-28 00:00:00
abstract::Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9365-9369.1998
更新日期:1998-11-01 00:00:00
abstract::The cellular tropism of the feline immunodeficiency virus (FIV) is affected by changes in variable region 3 (V3) of the surface (SU) envelope glycoprotein (Verschoor, E. J., et al., J. Virol. 69:4752-4757, 1995). By using high-dose DNA transfection, an FIV molecular clone with a non-CRFK-tropic V3 acquired the ability...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.7132-7135.1997
更新日期:1997-09-01 00:00:00
abstract:UNLABELLED:Identification and characterization of CD8(+) T cells effectively controlling HIV-1 variants are necessary for the development of AIDS vaccines and for studies of AIDS pathogenesis, although such CD8(+) T cells have been only partially identified. In this study, we sought to identify CD8(+) T cells controlli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00020-15
更新日期:2015-05-01 00:00:00
abstract::9-([2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) is more potent and has a more prolonged inhibitory effect against Epstein-Barr virus (EBV) in vitro than does acyclovir (ACV). To assess the mechanism of this difference, we first compared the extent of phosphorylation of the two drugs in superinfected Ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.569-573.1986
更新日期:1986-11-01 00:00:00
abstract::To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02728-07
更新日期:2008-06-01 00:00:00
abstract::We reported previously that the movement protein (MP) of tomato mosaic tobamovirus is phosphorylated, and we proposed that MP phosphorylation is important for viral pathogenesis. Experimental data indicated that phosphorylation enhances the stability of MP in vivo and enables the protein to assume the correct intracel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1452-1461.2003
更新日期:2003-01-01 00:00:00
abstract::The sequence of 2,191 nucleotides encoding the env gene of murine retrovirus Akv was determined by using a molecular clone of the Akv provirus. Deduction of the encoded amino acid sequence showed that a single open reading frame encodes a 638-amino acid precursor to gp70 and p15E. In addition, there is a typical leade...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.519-529.1982
更新日期:1982-05-01 00:00:00
abstract::The movement protein (MP) of Tobacco mosaic virus mediates the cell-to-cell transport of viral RNA through plasmodesmata, cytoplasmic cell wall channels for direct cell-to-cell communication between adjacent cells. Previous in vivo studies demonstrated that the RNA transport function of the protein correlates with its...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00254-06
更新日期:2006-06-01 00:00:00
abstract::The Epstein-Barr virus (EBV) major envelope glycoprotein gp340 is the subject of current efforts to develop an EBV subunit vaccine. The importance of gp340-specific humoral immunity has been highlighted by studies of natural infection in humans and gp340 immunization of experimental animals. The former studies have de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1246-1251.1992
更新日期:1992-02-01 00:00:00
abstract::While the facile transmission of chronic wasting disease (CWD) remains incompletely elucidated, studies in rodents suggest that exposure of the respiratory mucosa may be an efficient pathway. The present study was designed to address this question in the native cervid host. Here, we demonstrate aerosol transmission of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02852-12
更新日期:2013-02-01 00:00:00
abstract::The purified T-antigen origin binding domain binds site specifically to site II, the central region of the simian virus 40 core origin. However, in the context of full-length T antigen, the origin binding domain interacts poorly with DNA molecules containing just site II. Here we investigate the contributions of addit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.2921-2934.2004
更新日期:2004-03-01 00:00:00
abstract::Ebola virus (EBOV) causes severe hemorrhagic fever, for which therapeutic options are not available. Preventing the entry of EBOV into host cells is an attractive antiviral strategy, which has been validated for HIV by the FDA approval of the anti-HIV drug enfuvirtide. To identify inhibitors of EBOV entry, the EBOV en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01456-10
更新日期:2011-04-01 00:00:00
abstract::We used the mammalian reoviruses to determine the molecular basis of the clearance of a virus from the bloodstream by specific organs. Reovirus serotypes 1 (T1) and 3 (T3) were radiolabeled with [35S]methionine or 125I, and the viruses were injected intravenously into weanling rats. The distribution of radioactivity w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.545-551.1988
更新日期:1988-02-01 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
更新日期:1989-11-01 00:00:00