Abstract:
:Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active in an in vitro RNA polymerase assay using homopolymeric RNA or BVDV minigenomic RNA templates. The major product was a covalently linked double-stranded molecule generated by a "copy-back" mechanism from the input template RNA. In addition, a nucleotide-nonspecific and template-independent terminal nucleotidyl transferase activity was observed with the BVDV NS5B preparation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zhong W,Gutshall LL,Del Vecchio AMdoi
10.1128/JVI.72.11.9365-9369.1998subject
Has Abstractpub_date
1998-11-01 00:00:00pages
9365-9issue
11eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::The B19 parvovirus, a small single-stranded DNA virus of 5.4 kilobases, is pathogenic in humans. B19 has remarkable specificity for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2395-2406.1987
更新日期:1987-08-01 00:00:00
abstract::The identification of several simian immunodeficiency virus mac251 (SIV(mac251)) cytotoxic T-lymphocyte epitopes recognized by CD8(+) T cells of infected rhesus macaques carrying the Mamu-A*01 molecule and the use of peptide-major histocompatibility complex tetrameric complexes enable the study of the frequency, bread...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11483-11495.2001
更新日期:2001-12-01 00:00:00
abstract::The bovine papillomavirus type 1 (BPV-1) genome replicates as a multiple-copy plasmid in murine C127 cells transformed to neoplasia by virus infection or by transfection with BPV-1 DNA. It was reported previously that BPV-1 genomes harboring frameshift mutations in the E6 or E7 open reading frame (ORF) replicated in C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4957-4965.1992
更新日期:1992-08-01 00:00:00
abstract::The purified T-antigen origin binding domain binds site specifically to site II, the central region of the simian virus 40 core origin. However, in the context of full-length T antigen, the origin binding domain interacts poorly with DNA molecules containing just site II. Here we investigate the contributions of addit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.2921-2934.2004
更新日期:2004-03-01 00:00:00
abstract::The herpes simplex virus glycoprotein designated VP7(B2) is extracted from virions by nonionic detergent in the form of an oligomer, whereas the other detergent-soluble envelope proteins appear to be extracted as monomers. The subunits of the VP7(B2) oligomer cannot be dissociated by 2-mercaptoethanol and are also res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.1159-1167.1979
更新日期:1979-03-01 00:00:00
abstract::Engineered recombinant viruses expressing reporter genes have been developed for real-time monitoring of replication and for mass screening of antiviral inhibitors. Recently, we reported using a reverse genetics system to develop the first recombinant reporter rotaviruses (RVs) that expressed NanoLuc (NLuc) luciferase...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01774-18
更新日期:2019-02-05 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) typically displays two different phases in its life cycle, the default latent phase and the lytic phase. There is a short period of lytic gene expression in the early stage of KSHV primary infection. The factors involved in the shutdown process of lytic gene exp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-14
更新日期:2014-07-01 00:00:00
abstract::The ecotropic Cas-Br-E murine leukemia virus (MuLV) and its molecularly cloned derivative pBR-NE-8 MuLV are capable of inducing hind-limb paralysis and leukemia after inoculation into susceptible mice. T1 oligonucleotide fingerprinting, molecular hybridization, and restriction enzyme analysis previously showed that th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.910-919.1986
更新日期:1986-12-01 00:00:00
abstract::Influenza A virus NS2 protein, also called nuclear export protein (NEP), is crucial for the nuclear export of viral ribonucleoproteins. However, the molecular mechanisms of NEP mediation in this process remain incompletely understood. A leucine-rich nuclear export signal (NES2) in NEP, located at the predicted N2 heli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06519-11
更新日期:2013-01-01 00:00:00
abstract::Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. In the present study, we have investigat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1764-1771.2003
更新日期:2003-02-01 00:00:00
abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
abstract::The dengue type 4 virus (DEN4) genome contains a 384-nucleotide (nt) 3' noncoding sequence in which the last 81 nt, predicted to form a secondary structure, are thought to be essential for virus replication. Immediately upstream of the secondary structure, short RNA sequences that are conserved among mosquito-borne fl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
更新日期:1996-06-01 00:00:00
abstract::Recombinant Epstein-Barr viruses (EBVs) were made with mutated latent membrane protein 1 (LMP1) genes that express only the LMP1 amino-terminal cytoplasmic and six transmembrane domains (MS187) or these domains and the first 44 amino acids of the 200-residue LMP1 carboxy-terminal domain (MS231). After infection of pri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.675-683.1995
更新日期:1995-02-01 00:00:00
abstract::Two murine leukemia viruses were isolated from JLS-V9 cells which had been infected with Rauscher plasma virus. One virus was XC positive and failed to grow on mink or cat cells and thus was an ecotropic virus. The other virus formed cytopathic foci on mink cells, was XC negative, and fell into the mink cell focus-for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.3.995-1003.1983
更新日期:1983-03-01 00:00:00
abstract::UR2 is a newly characterized avian sarcoma virus whose genome contains a unique sequence that is not related to the sequences of other avian sarcoma virus transforming genes thus far identified. This unique sequence, termed ros, is fused to part of the viral gag gene. The product of the fused gag-ros gene of UR2 is a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.228-236.1982
更新日期:1982-04-01 00:00:00
abstract::To explore the roles played by specific human immunodeficiency virus type 1 (HIV-1) genes in determining the in vivo replicative capacity of AIDS viruses, we have examined the replication kinetics and virus-specific immune responses in rhesus monkeys following infection with two chimeric simian/human immunodeficiency ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3198-3206.1996
更新日期:1996-05-01 00:00:00
abstract::We have examined the pathway of rhinovirus interaction with soluble intercellular adhesion molecule 1 (sICAM-1). Binding of sICAM-1 to rhinovirus serotypes 3 and 14 gives particles with sedimentation coefficients from 145 to 120S, depending on the amount of sICAM-1 bound. The formation of 120S particles is faster and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5882-5889.1994
更新日期:1994-09-01 00:00:00
abstract::Four mutants of the virulent Mahoney strain of poliovirus were generated by introducing mutations in nucleotides (nt) 128 to 134 of the genome, a region that contains a part of the stem-loop II (SLII) structure located within the internal ribosomal entry site (IRES; nt 120 to 590) (K. Shiroki, T. Ishii, T. Aoki, Y. Ot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2398-2405.1998
更新日期:1998-03-01 00:00:00
abstract::The influenza A virus (IAV) M2 protein is a multifunctional protein with critical roles in virion entry, assembly, and budding. M2 is targeted to the apical plasma membrane of polarized epithelial cells, and the interaction of the viral proteins M2, M1, HA, and NA near glycolipid rafts in the apical plasma membrane is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01425-18
更新日期:2018-10-29 00:00:00
abstract::C57BL mice (Fv-1b) develop a severe immunodeficiency disease following inoculation as adults with LP-BM5 murine leukemia virus (MuLV), a derivative of Duplan-Laterjet virus which contains B-tropic ecotropic and mink cell focus-inducing MuLVs and a putative defective genome which may be the proximal cause of disease. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1223-1231.1989
更新日期:1989-03-01 00:00:00
abstract::Zika virus (ZIKV) envelope (E) protein is the major target of neutralizing antibodies in infected hosts and thus represents a candidate of interest for vaccine design. However, a major concern in the development of vaccines against ZIKV and the related dengue virus is the induction of cross-reactive poorly neutralizin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-20
更新日期:2020-12-09 00:00:00
abstract::We previously demonstrated that polyhedron formation (PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovirus (LdMNPV) in the L. dispar 652Y cell line (J. M. Slavicek, N. Hayes-Plazolles, and M. E. Kelly, Biol. Control 5:251-261, 1995). Most of these PF mutants exhibite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1097-1106.1997
更新日期:1997-02-01 00:00:00
abstract::Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.464-467.1991
更新日期:1991-01-01 00:00:00
abstract::Influenza A virus matrix protein M1 is involved in multiple stages of the viral infectious cycle. Despite its functional importance, our present understanding of this essential viral protein is limited. The roles of a small subset of specific amino acids have been reported, but a more comprehensive understanding of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00161-19
更新日期:2019-06-14 00:00:00
abstract::Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00358-12
更新日期:2012-08-01 00:00:00
abstract::RNA structures play key roles in the replication of RNA viruses. Sequence alignment software, thermodynamic RNA folding programs, and classical comparative phylogenetic analysis were used to build models of six RNA elements in the coding region of the hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B. The imp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1352-1366.2004
更新日期:2004-02-01 00:00:00
abstract::Structural proteins designated p10gag, p21gag, p8gag, p3gag, p27gag, and p14gag from the C3H strain of mouse mammary tumor virus (MMTV) were purified by reversed-phase high-pressure liquid chromatography. The N- and C-terminal amino acid sequences and amino acid composition of each protein were determined and compared...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2543-2549.1989
更新日期:1989-06-01 00:00:00
abstract::A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte gro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.5068-5074.1993
更新日期:1993-08-01 00:00:00
abstract::In vivo decay rates for the individual T7 early mRNA species were determined. The physical half-lives, measured at 37 C, range from 1.1 min for gene 0.7 RNA to 4.5 min for gene 0.3 RNA. Physical half-lives, as observed after rifampin inhibition of RNA synthesis and polyacylamide electrophoresis of RNAs, are approximat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.2.642-658.1976
更新日期:1976-02-01 00:00:00
abstract::The induction of alpha/beta interferon (IFN-alpha/beta) genes constitutes one of the first responses of the cell to virus infection. The IFN-beta gene is constitutively repressed in uninfected cells and is transiently activated after virus infection. In this work we demonstrate that histone deacetylation regulates the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3444-3452.2001
更新日期:2001-04-01 00:00:00