Abstract：
:Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. In the present study, we have investigated the role of capsid phosphorylation in virus replication. We have identified a single serine residue within the RNA binding region that is required for normal phosphorylation of this protein. The importance of capsid phosphorylation in virus replication was demonstrated by the fact that recombinant viruses encoding hypophosphorylated capsids replicated at much lower titers and were less cytopathic than wild-type virus. Nonphosphorylated mutant capsid proteins exhibited higher affinities for viral RNA than wild-type phosphorylated capsids. Capsid protein isolated from wild-type strain virions bound viral RNA more efficiently than cell-associated capsid. However, the RNA-binding activity of cell-associated capsids increased dramatically after treatment with phosphatase, suggesting that the capsid is dephosphorylated during virus assembly. In vitro assays indicate that the capsid may be a substrate for protein phosphatase 1A. As capsid is heavily phosphorylated under conditions where virus assembly does not occur, we propose that phosphorylation serves to negatively regulate binding of viral genomic RNA. This may delay the initiation of nucleocapsid assembly until sufficient amounts of virus glycoproteins accumulate at the budding site and/or prevent nonspecific binding to cellular RNA when levels of genomic RNA are low. It follows that at a late stage in replication, the capsid may undergo dephosphorylation before nucleocapsid assembly occurs.
journal_name
J Viroljournal_title
Journal of virologyauthors
Law LM,Everitt JC,Beatch MD,Holmes CF,Hobman TCdoi
10.1128/jvi.77.3.1764-1771.2003subject
Has Abstractpub_date
2003-02-01 00:00:00pages
1764-71issue
3eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract：:Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.12.8558-8563.1996
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abstract：:Nucleotide sequences were determined for the complete M genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and PCR-positive white-footed mice (Peromyscus leucopus) from Indiana and Oklahoma. Phylogenetic analyses indicated that although divergent from each other, th...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.1.57-64.1998
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1128/JVI.01240-07
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01415-20
更新日期：2020-12-09 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.8.4442-4448.1991
更新日期：1991-08-01 00:00:00
abstract：:The first morphological evidence of African swine fever virus (ASFV) assembly is the appearance of precursor viral membranes, thought to derive from the endoplasmic reticulum, within the assembly sites. We have shown previously that protein p54, a viral structural integral membrane protein, is essential for the genera...
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pub_type： 杂志文章
doi：10.1128/JVI.00600-10
更新日期：2010-08-01 00:00:00
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doi：10.1128/JVI.02727-12
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.6.4571-4579.1998
更新日期：1998-06-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.68.9.5365-5374.1994
更新日期：1994-09-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.5.2861-2868.1996
更新日期：1996-05-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00650-12
更新日期：2012-07-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.48.3.752-764.1983
更新日期：1983-12-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02107-16
更新日期：2017-01-18 00:00:00
abstract：:Hepatitis C virus (HCV) is an important human pathogen associated with chronic liver disease. Recently, based on a genotype 2a isolate, tissue culture systems supporting complete replication and infectious virus production have been developed. In this study, we used cell culture-produced infectious HCV to analyze the ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.80.4.1734-1741.2006
更新日期：2006-02-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.79.7.4090-4098.2005
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journal_title：Journal of virology
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pub_type： 杂志文章
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journal_title：Journal of virology
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journal_title：Journal of virology
pub_type： 杂志文章,评审
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更新日期：2014-08-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.12.2.397-400.1973
更新日期：1973-08-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.66.10.6233-6236.1992
更新日期：1992-10-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.29.2.612-623.1979
更新日期：1979-02-01 00:00:00
abstract：:In patients with impaired cell-mediated immune responses (e.g., lung transplant recipients and AIDS patients), cytomegalovirus (CMV) infection causes severe disease such as pneumonitis. However, although immunocompetency in the host can protect from CMV disease, the virus persists by evading the host immune defenses. ...
journal_title：Journal of virology
pub_type： 杂志文章
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更新日期：1998-07-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
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更新日期：2010-11-01 00:00:00
abstract：:Recent evidence supports a role for vitamin K-dependent coagulation zymogens in adenovirus serotype 5 (Ad5, subgroup C) infection of hepatocytes. Here, we assessed the effect of virus-zymogen interaction on cellular transduction using a panel of fiber (f)-pseudotyped viruses derived from subgroup D (f47, f33, f24, f45...
journal_title：Journal of virology
pub_type： 杂志文章
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更新日期：2007-04-01 00:00:00
abstract：:CM2 is the second membrane protein of the influenza C virus and has been demonstrated to play a role in the uncoating and genome packaging processes in influenza C virus replication. Although the effects of N-linked glycosylation, disulfide-linked oligomerization, and palmitoylation of CM2 on virus replication have be...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00773-17
更新日期：2017-10-27 00:00:00
