Abstract:
:The envelope glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) is posttranslationally cleaved into two subunits. We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By systematic mutational analysis of the LCMV GP cleavage site, we determined that the consensus motif R-(R/K/H)-L-(A/L/S/T/F)(265) is essential for the endoproteolytic processing. In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1/S1P cleaves LCMV GP.
journal_name
J Viroljournal_title
Journal of virologyauthors
Beyer WR,Pöpplau D,Garten W,von Laer D,Lenz Odoi
10.1128/jvi.77.5.2866-2872.2003subject
Has Abstractpub_date
2003-03-01 00:00:00pages
2866-72issue
5eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
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