Phagocytosis of picornavirus-infected cells induces an RNA-dependent antiviral state in human dendritic cells.


:Dendritic cells (DCs) play a central role in instructing antiviral immune responses. DCs, however, can become targeted by different viruses themselves. We recently demonstrated that human DCs can be productively infected with echoviruses (EVs), but not coxsackie B viruses (CVBs), both of which are RNA viruses belonging to the Enterovirus genus of the Picornaviridae family. We now show that phagocytosis of CVB-infected, type I interferon-deficient cells induces an antiviral state in human DCs. Uptake of infected cells increased the expression of the cytoplasmic RNA helicases retinoic acid-inducible gene I and melanoma differentiation-associated gene 5 as well as other interferon-stimulated genes and protected DCs against subsequent infection with EV9. These effects depended on recognition of viral RNA and could be mimicked by exposure to the synthetic double-stranded RNA analogue poly(I:C) but not other Toll-like receptor (TLR) ligands. Blocking endosomal acidification abrogated protection, suggesting a role for TLRs in the acquisition of an antiviral state in DCs. In conclusion, recognition of viral RNA rapidly induces an antiviral state in human DCs. This might provide a mechanism by which DCs protect themselves against viruses when attracted to an environment with ongoing infection.


J Virol


Journal of virology


Kramer M,Schulte BM,Toonen LW,Barral PM,Fisher PB,Lanke KH,Galama JM,van Kuppeveld FJ,Adema GJ




Has Abstract


2008-03-01 00:00:00














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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Thomas JA,Bosche WJ,Shatzer TL,Johnson DG,Gorelick RJ

    更新日期:2008-10-01 00:00:00

  • A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs.

    abstract::An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template int...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Fodor E,Mingay LJ,Crow M,Deng T,Brownlee GG

    更新日期:2003-04-01 00:00:00

  • Restitution of infectivity to spikeless vesicular stomatitis virus by solubilized viral components.

    abstract::Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bishop DH,Repik P,Obijeski JF,Moore NF,Wagner RR

    更新日期:1975-07-01 00:00:00

  • Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7.

    abstract::The steady-state level and metabolic half-life of retinoblastoma tumor suppressor protein pRB are decreased in cells that express high-risk human papillomavirus (HPV) E7 proteins. Here we show that pRB degradation is a direct activity of E7 and does not reflect a property of cell lines acquired during the selection pr...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gonzalez SL,Stremlau M,He X,Basile JR,Münger K

    更新日期:2001-08-01 00:00:00

  • Hantaan virus infection of human endothelial cells.

    abstract::The primary pathophysiologic finding of the viral disease known as Korean hemorrhagic fever, the etiological agent of which is Hantaan virus (HTV), is vascular instability. To investigate whether HTV was able to infect cells derived from human vascular tissue and alter their behavior, we infected in vitro primary adul...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pensiero MN,Sharefkin JB,Dieffenbach CW,Hay J

    更新日期:1992-10-01 00:00:00

  • The ability of an oligomeric human immunodeficiency virus type 1 (HIV-1) envelope antigen to elicit neutralizing antibodies against primary HIV-1 isolates is improved following partial deletion of the second hypervariable region.

    abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Barnett SW,Lu S,Srivastava I,Cherpelis S,Gettie A,Blanchard J,Wang S,Mboudjeka I,Leung L,Lian Y,Fong A,Buckner C,Ly A,Hilt S,Ulmer J,Wild CT,Mascola JR,Stamatatos L

    更新日期:2001-06-01 00:00:00

  • Cocirculation of avian H9N2 and contemporary "human" H3N2 influenza A viruses in pigs in southeastern China: potential for genetic reassortment?

    abstract::Pigs are permissive to both human and avian influenza viruses and have been proposed to be an intermediate host for the genesis of pandemic influenza viruses through reassortment or adaptation of avian viruses. Prospective virological surveillance carried out between March 1998 and June 2000 in Hong Kong, Special Admi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Peiris JS,Guan Y,Markwell D,Ghose P,Webster RG,Shortridge KF

    更新日期:2001-10-01 00:00:00

  • Recombination between endogenous and exogenous simian virus 40 genes. II. Biochemical evidence for genetic exchange.

    abstract::The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol. 24:534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue o...

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    pub_type: 杂志文章


    authors: Vogel T,Gluzman Y,Winocour E

    更新日期:1977-11-01 00:00:00

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    abstract::The mechanism of regulation for the ribozyme activity of hepatitis delta virus (HDV) RNA in infected cells is unknown. Previously, we developed a direct assay capable of detecting the ribozyme activity of HDV dimer or trimer RNAs in vivo (K.-S. Jeng, A. Daniel, and M. M. C. Lai, J. Virol, 70:2403-2410, 1996). In this ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jeng KS,Su PY,Lai MM

    更新日期:1996-07-01 00:00:00

  • CXCR4 expression during lymphopoiesis: implications for human immunodeficiency virus type 1 infection of the thymus.

    abstract::Human immunodeficiency virus type 1 (HIV-1) infection of the human thymus results in depletion of CD4-bearing thymocytes. This depletion is initially manifested in the immature CD4+/CD8+ thymocyte subset. To determine cellular factors involved in HIV infection in the thymus, we examined the expression of the recently ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kitchen SG,Zack JA

    更新日期:1997-09-01 00:00:00

  • The majority of freshly sorted simian immunodeficiency virus (SIV)-specific CD8(+) T cells cannot suppress viral replication in SIV-infected macrophages.

    abstract::Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) primarily infect activated CD4(+) T cells but can infect macrophages. Surprisingly, ex vivo tetramer-sorted SIV-specific CD8(+) T cells that eliminated and suppressed viral replication in SIV-infected CD4(+) T cells failed to do so in SIV-infec...

    journal_title:Journal of virology

    pub_type: 杂志文章


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    更新日期:2012-04-01 00:00:00

  • Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1.

    abstract::The major DNA-binding protein encoded by several temperature-sensitive mutants of herpes simplex virus type 1 was thermolabile for binding to intracellular viral DNA. The ability of DNase I to release this protein from isolated nuclei was used as a measure of the amount of protein bound to viral DNA. This assay was ba...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lee CK,Knipe DM

    更新日期:1983-06-01 00:00:00

  • Proteins of the group B arbovirus Kunjin.

    abstract::Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the viri...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Westaway EG,Reedman BM

    更新日期:1969-11-01 00:00:00

  • Covalently closed circular DNA is the predominant form of duck hepatitis B virus DNA that persists following transient infection.

    abstract::Residual hepatitis B virus (HBV) DNA can be detected in serum and liver after apparent recovery from transient infection. However, it is not known if this residual HBV DNA represents ongoing viral replication and antigen expression. In the current study, ducks inoculated with duck hepatitis B virus (DHBV) were monitor...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Le Mire MF,Miller DS,Foster WK,Burrell CJ,Jilbert AR

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Utermöhlen O,Dangel A,Tárnok A,Lehmann-Grube F

    更新日期:1996-03-01 00:00:00

  • Role of SP1-binding domains in in vivo transcriptional regulation of the human immunodeficiency virus type 1 long terminal repeat.

    abstract::Five regions of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) have been shown to be important in the transcriptional regulation of HIV in HeLa cells. These include the negative regulatory, enhancer, SP1, TATA, and TAR regions. Previous studies in which purified SP1 was used showed that the...

    journal_title:Journal of virology

    pub_type: 杂志文章


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    更新日期:1989-06-01 00:00:00

  • Th-cytotoxic T-lymphocyte chimeric epitopes extended by Nepsilon-palmitoyl lysines induce herpes simplex virus type 1-specific effector CD8+ Tc1 responses and protect against ocular infection.

    abstract::Molecularly defined vaccine formulations capable of inducing antiviral CD8+ T-cell-specific immunity in a manner compatible with human delivery are limited. Few molecules achieve this target without the support of an appropriate immunological adjuvant. In this study, we investigate the potential of totally synthetic p...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zhang X,Issagholian A,Berg EA,Fishman JB,Nesburn AB,BenMohamed L

    更新日期:2005-12-01 00:00:00

  • Mapping of B-cell epitopes on the polypeptide chain of the Epstein-Barr virus major envelope glycoprotein and candidate vaccine molecule gp340.

    abstract::The Epstein-Barr virus (EBV) major envelope glycoprotein gp340 is the subject of current efforts to develop an EBV subunit vaccine. The importance of gp340-specific humoral immunity has been highlighted by studies of natural infection in humans and gp340 immunization of experimental animals. The former studies have de...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Pither RJ,Zhang CX,Shiels C,Tarlton J,Finerty S,Morgan AJ

    更新日期:1992-02-01 00:00:00

  • A vesiculovirus showing a steepened transcription gradient and dominant trans-repression of virus transcription.

    abstract::Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hodges EN,Heinrich BS,Connor JH

    更新日期:2012-08-01 00:00:00

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    abstract::In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Edinger AL,Ahuja M,Sung T,Baxter KC,Haggarty B,Doms RW,Hoxie JA

    更新日期:2000-09-01 00:00:00

  • A recombinant newcastle disease virus with low-level V protein expression is immunogenic and lacks pathogenicity for chicken embryos.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mebatsion T,Verstegen S,De Vaan LT,Römer-Oberdörfer A,Schrier CC

    更新日期:2001-01-01 00:00:00

  • Three arginine residues within the RGG box are crucial for ICP27 binding to herpes simplex virus 1 GC-rich sequences and for efficient viral RNA export.

    abstract::ICP27 is a multifunctional protein that is required for herpes simplex virus 1 mRNA export. ICP27 interacts with the mRNA export receptor TAP/NXF1 and binds RNA through an RGG box motif. Unlike other RGG box proteins, ICP27 does not bind G-quartet structures but instead binds GC-rich sequences that are flexible in str...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Corbin-Lickfett KA,Souki SK,Cocco MJ,Sandri-Goldin RM

    更新日期:2010-07-01 00:00:00

  • Stem Cell Properties of Normal Human Keratinocytes Determine Transformation Responses to Human Papillomavirus 16 DNA.

    abstract::Human papillomavirus (HPV) infection of the genital tract is common; however, only about 10 to 15% of infections persist, and approximately 10 to 15% of these persistent infections result in cancer. Basal epidermal stem cells are the presumed target cells for HPV infection, providing a reservoir of latently infected c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Woappi Y,Hosseinipour M,Creek KE,Pirisi L

    更新日期:2018-05-14 00:00:00

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    abstract::Toroviruses (family Coronaviridae, order Nidovirales) are enveloped, positive-stranded RNA viruses that have been implicated in enteric disease in cattle and possibly in humans. Despite their potential veterinary and clinical relevance, little is known about torovirus epidemiology and molecular genetics. Here, we pres...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Smits SL,Lavazza A,Matiz K,Horzinek MC,Koopmans MP,de Groot RJ

    更新日期:2003-09-01 00:00:00

  • Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.

    abstract::Retrovirus particles each contain two copies of the viral genome in the form of a noncovalently linked RNA dimer. Earlier studies have mapped a cis-acting region near the 5' end of the human immunodeficiency virus type 1 (HIV-1) genome, termed the psi locus, which appears essential for initiation of genomic dimerizati...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Clever JL,Parslow TG

    更新日期:1997-05-01 00:00:00

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    abstract::Rio Bravo virus (RBV) is a member of the family Flaviviridae, genus Flavivirus. It belongs to a group of viruses in the genus with no known vector. In this report, we analyze the complete genome of the prototype RBV, strain M64. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Volkova E,Tesh RB,Monath TP,Vasilakis N

    更新日期:2012-04-01 00:00:00

  • The integrity of the stem structure of human immunodeficiency virus type 1 Tat-responsive sequence of RNA is required for interaction with the interferon-induced 68,000-Mr protein kinase.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Roy S,Agy M,Hovanessian AG,Sonenberg N,Katze MG

    更新日期:1991-02-01 00:00:00

  • Evolutionarily conserved function of a viral microRNA.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Seo GJ,Fink LH,O'Hara B,Atwood WJ,Sullivan CS

    更新日期:2008-10-01 00:00:00

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    abstract::Borna disease virus (BDV) is an enveloped virus with a genome organization characteristic of Mononegavirales. However, based on its unique features, BDV is considered the prototypic member of a new virus family, Bornaviridae, within the order Mononegavirales. We have described the establishment of a reverse genetics s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rosario D,Perez M,de la Torre JC

    更新日期:2005-05-01 00:00:00

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    abstract::We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes. DNA containing the hybrid genes was transfected into chicken cells together with viral vector DNA and helper viral DNA, an...

    journal_title:Journal of virology

    pub_type: 杂志文章


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    更新日期:1985-12-01 00:00:00