Abstract：
:A poliovirus-specific polyuridylic acid [poly(U)] polymerase that copies a polyadenylic acid template complexed to an oligouridylic acid primer was isolated from the membrane fraction of infected HeLa cells and was found to sediment at 4 to 5S on a linear 5 to 20% glycerol gradient. When the poly(U) polymerase was isolated from cells labeled with [(35)S]methionine and was analyzed by glycerol gradient centrifugation and polyacrylamide gel electrophoresis, the position of only one viral protein was found to correlate with the location of enzyme activity. This protein had an apparent molecular weight of 62,500 based on its electrophoretic mobility relative to that of several molecular weight standards and was designated p63. When the poly(U) polymerase was isolated from the soluble fraction of a cytoplasmic extract, the activity was found to sediment at about 7S. In this case, however, both p63 and NCVP2 (77,000-dalton precursor of p63) cosedimented with the 7S activity peak. When the 7S polymerase activity was purified by phosphocellulose chromatography, both p63 and NCVP2 were found to co-chromatograph with poly(U) polymerase activity. The poliovirus replicase complexed with its endogenous RNA template was isolated from infected cells labeled with [(35)S]methionine and was centrifuged through a linear 15 to 30% glycerol gradient. The major viral polypeptide component in a 26S peak of replicase activity was p63, but small amounts of other poliovirus proteins were also present. When the replicase-template complex was treated with RNase T1 before centrifugation, a single peak of activity was found that sedimented at 20S and contained only labeled p63. Thus, p63 was found to be the only viral polypeptide in the replicase bound to its endogenous RNA template, and appears to be active as a poly(U) polymerase either as a monomer protein or as a 7S complex.
journal_name
J Viroljournal_title
Journal of virologyauthors
Flanegan JB,Baltimore Ddoi
10.1128/JVI.29.1.352-360.1979subject
Has Abstractpub_date
1979-01-01 00:00:00pages
352-60issue
1eissn
0022-538Xissn
1098-5514journal_volume
29pub_type
杂志文章abstract：:As potential targets for human immunodeficiency virus type 1 and simian immunodeficiency virus (HIV-1 and SIV), dendritic cells (DCs) likely play a significant role in the onset and spread of infection as well as in the induction of antiviral immunity. Using the SIV-macaque system to study the very early events in DC-...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.76.6.2936-2951.2002
更新日期：2002-03-01 00:00:00
abstract：UNLABELLED:Like most positive-strand RNA viruses, infection by plant tombusviruses results in extensive rearrangement of specific host cell organelle membranes that serve as the sites of viral replication. The tombusvirus Tomato bushy stunt virus (TBSV) replicates within spherules derived from the peroxisomal boundary ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.03840-13
更新日期：2014-06-01 00:00:00
abstract：:Tobacco necrosis virus, strain D (TNV-D), is a positive-strand RNA virus in the genus Betanecrovirus and family Tombusviridae The production of its RNA-dependent RNA polymerase, p82, is achieved by translational readthrough. This process is stimulated by an RNA structure that is positioned immediately downstream of th...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02443-16
更新日期：2017-03-29 00:00:00
abstract：:A virus structurally similar to viruses associated with maedi, progressive pneumonia, and visna of sheep has been isolated from buffy coat cells of cattle with chronic lymphocytosis. Electron microscope studies revealed three variants of the virion: (i) an intracytoplasmic form 98 to 116 nm in diameter when occurring ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.13.1.197-204.1974
更新日期：1974-01-01 00:00:00
abstract：:The signaling lymphocyte activation molecule F1 (SLAMF1) is both a microbial sensor and entry receptor for measles virus (MeV). Herein, we describe a new role for SLAMF1 to mediate MeV endocytosis that is in contrast with the alternative, and generally accepted, model that MeV genome enters cells only after fusion at ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02255-16
更新日期：2017-03-13 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02686-05
更新日期：2006-08-01 00:00:00
abstract：:A murine retrovirus encoding the middle T protein of polyomavirus infected and transformed nonestablished chicken embryo cells. The infected cultures formed colonies in soft agar-containing medium and released infectious transforming virus. Middle T protein expressed in the transformed chicken cells associated with p6...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.56.3.1023-1026.1985
更新日期：1985-12-01 00:00:00
abstract：:Picornaviruses are a family of positive-strand RNA viruses that are responsible for a variety of devastating human and animal diseases. An attenuated strain of mengovirus (vMC24) is serologically indistinguishable from the lethal murine wild-type mengovirus and encephalomyocarditis virus (EMCV). Immunogen-specific sti...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.8.4914-4923.1995
更新日期：1995-08-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.07046-11
更新日期：2012-03-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.16.6.1391-1400.1975
更新日期：1975-12-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00823-11
更新日期：2011-11-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.77.22.12033-12047.2003
更新日期：2003-11-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.63.9.3622-3633.1989
更新日期：1989-09-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02231-07
更新日期：2008-07-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.8.3642-3649.2000
更新日期：2000-04-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01259-13
更新日期：2013-09-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00537-18
更新日期：2018-07-17 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.1.4.738-746.1967
更新日期：1967-08-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.12.6998-7003.1991
更新日期：1991-12-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.62.10.3867-3869.1988
更新日期：1988-10-01 00:00:00
abstract：:Brome mosaic virus (BMV), a member of the alphavirus-like super-family of positive-strand RNA viruses, encodes two proteins required for viral RNA replication: 1a and 2a. 1a contains m7G methyltransferase- and helicase-like domains, while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal extensions...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.70.7.4729-4736.1996
更新日期：1996-07-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.3.1725-1730.1998
更新日期：1998-03-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.6.3889-3892.1995
更新日期：1995-06-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.3.5.477-483.1969
更新日期：1969-05-01 00:00:00
abstract：:Recently, efficient and long-term in vivo gene transfer by recombinant adeno-associated virus type 2 (rAAV) vectors has been demonstrated in a variety of tissues. Further improvement in vector titer and purity will expedite this in vivo exploration and provide preclinical information required for use in human gene the...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.3.2224-2232.1998
更新日期：1998-03-01 00:00:00
abstract：:Cytomegalovirus (CMV) infection is the most common opportunistic infection in immunosuppressed individuals, such as transplant recipients or people living with HIV/AIDS, and congenital CMV is the leading viral cause of developmental disabilities in infants. Due to the highly species-specific nature of CMV, animal mode...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.05840-11
更新日期：2011-12-01 00:00:00
abstract：:The latent membrane protein 1 (LMP-1) of Epstein-Barr virus (EBV) functionally resembles a constitutively active, CD40-like receptor and contributes to the maintenance of proliferation of EBV-infected primary human B lymphocytes. LMP-1 is targeted to the plasma membrane, where it binds TRAF, TRADD, and JAK molecules t...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.20.9755-9761.2000
更新日期：2000-10-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00106-07
更新日期：2007-08-01 00:00:00
abstract：:The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) initiates apoptosis in diverse insects through events triggered by virus DNA (vDNA) replication. To define the proapoptotic pathway and its role in antivirus defense, we investigated the link between the host's DNA damage response (DDR) a...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02246-12
更新日期：2012-12-01 00:00:00
abstract：:Human MxA protein is an interferon-induced 76-kDa GTPase that exhibits antiviral activity against several RNA viruses. Wild-type MxA accumulates in the cytoplasm of cells. TMxA, a modified form of wild-type MxA carrying a foreign nuclear localization signal, accumulates in the cell nucleus. Here we show that MxA prote...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.4.2591-2599.1997
更新日期：1997-04-01 00:00:00
