Abstract:
:To map the DNA-binding domain of polyomavirus large T antigen, we constructed a set of plasmids coding for unidirectional carboxy- or amino-terminal deletion mutations in the large T antigen. Analysis of origin-specific DNA binding by mutant proteins expressed in Cos-1 cells revealed that the C-terminal boundary of the DNA-binding domain is at or near Glu-398. Fusion proteins of large T antigen lacking the first 200 N-terminal amino acids bound specifically to polyomavirus origin DNA; however, deletions beyond this site resulted in unstable proteins which could not be tested for DNA binding. Testing of point mutants and internal deletions by others suggested that the N-terminal boundary of the DNA-binding domain lies between amino acids 282 and 286. Taken together, these results locate the DNA-binding domain of polyomavirus large T antigen to the 116-amino-acid region between residues 282 and 398.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sunstrom NA,Acheson NH,Hassell JAdoi
10.1128/JVI.65.12.6998-7003.1991subject
Has Abstractpub_date
1991-12-01 00:00:00pages
6998-7003issue
12eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
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doi:10.1128/JVI.64.11.5412-5419.1990
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pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9844-9854.1998
更新日期:1998-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.664-671.1991
更新日期:1991-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1559-1568.2002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3530-3537.1991
更新日期:1991-07-01 00:00:00
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更新日期:1979-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1967-06-01 00:00:00
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更新日期:2004-10-01 00:00:00