Abstract:
:Many complex viruses use an assembly pathway in which their genome is packaged into an empty procapsid which subsequently matures into its final expanded form. We utilized Pseudomonas phage 6, a well-established virus assembly model, to probe the plasticity of the procapsid maturation pathway. The 6 packaging nucleoside triphosphatase (NTPase), which powers sequential translocation of the three viral genomic single-stranded RNA molecules to the procapsid during capsid maturation, is part of the mature 6 virion but may spontaneously be dissociated from the procapsid shell. We demonstrate that the dissociation of NTPase subunits results in premature capsid expansion, which is detected as a change in the sedimentation velocity and as defects in RNA packaging and transcription activity. However, this dead-end conformation of the procapsids was rescued by the addition of purified NTPase hexamers, which efficiently associated on the NTPase-deficient particles and subsequently drove their contraction to the compact naive conformation. The resulting particles regained their biological and enzymatic activities, directing them into a productive maturation pathway. These observations imply that the maturation pathways of complex viruses may contain reversible steps that allow the rescue of the off-pathway conformation in an overall unidirectional virion assembly pathway. Furthermore, we provide direct experimental evidence that particles which have different physical properties (distinct sedimentation velocities and conformations) display different stages of the genome packaging program and show that the transcriptional activity of the 6 procapsids correlates with the number of associated NTPase subunits.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sun X,Pirttimaa MJ,Bamford DH,Poranen MMdoi
10.1128/JVI.02285-13subject
Has Abstractpub_date
2013-12-01 00:00:00pages
13279-86issue
24eissn
0022-538Xissn
1098-5514pii
JVI.02285-13journal_volume
87pub_type
杂志文章abstract:UNLABELLED:Equine influenza viruses (EIV) are responsible for rapidly spreading outbreaks of respiratory disease in horses. Although natural infections of humans with EIV have not been reported, experimental inoculation of humans with these viruses can lead to a productive infection and elicit a neutralizing antibody r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02449-14
更新日期:2015-02-01 00:00:00
abstract::In vitro transcription by vesicular stomatitis virus nucleocapsids is inhibited by enzymatic dephosphorylation of the NS protein. We provide evidence that specific, partial dephosphorylation of NS molecules is the only detectable change in nucleocapsids treated with bacterial alkaline phosphatase under conditions that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.104-112.1982
更新日期:1982-07-01 00:00:00
abstract::Influenza virus defective interfering (DI) particles are naturally occurring noninfectious virions typically generated during in vitro serial passages in cell culture of the virus at a high multiplicity of infection. DI particles are recognized for the role they play in inhibiting viral replication and for the impact ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00240-13
更新日期:2013-07-01 00:00:00
abstract::IgG1 b12 is a broadly neutralizing antibody against human immunodeficiency virus type 1 (HIV-1). The epitope recognized by b12 overlaps the CD4 receptor-binding site (CD4bs) on gp120 and has been a target for vaccine design. Determination of the three-dimensional structure of immunoglobulin G1 (IgG1) b12 allowed model...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5863-5876.2003
更新日期:2003-05-01 00:00:00
abstract::The distantly related lentiviruses human immunodeficiency virus type 1 (HIV-1) and visna virus each encode a posttranscriptional regulatory protein, termed Rev, that is critical for expression of the viral structural proteins. We genetically mapped the cis-acting target sequence for visna virus Rev, the visna virus Re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3609-3615.1992
更新日期:1992-06-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is frequently associated with extrahepatic manifestations, including nonmalignant and malignant B-cell lymphoproliferative disorders. It has been reported that specific changes or recurring motifs in the amino acid sequence of the HCV hypervariable region 1 (HVR1) may be assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-06
更新日期:2007-05-01 00:00:00
abstract::To investigate the genetic stability of vaccinia virus DNA, we have tested whether alterations occurred in the polypeptide composition of this complex virus during persistent infections. We found that variants isolated at various passages in Friend erythroleukemia cells persistently infected with vaccinia virus contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2642-2647.1987
更新日期:1987-08-01 00:00:00
abstract::The binding specificities of a panel of avian influenza virus subtype H5 hemagglutinin (HA) proteins bearing mutations at key residues in the receptor binding site were investigated. The results demonstrate that two simultaneous mutations in the receptor binding site resulted in H5 HA binding in a pattern similar to t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.502-507.2004
更新日期:2004-01-01 00:00:00
abstract:UNLABELLED:Influenza B virus causes annual epidemics and, along with influenza A virus, accounts for substantial disease and economic burden throughout the world. Influenza B virus infects only humans and some marine mammals and is not responsible for pandemics, possibly due to a very low frequency of reassortment and ...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
abstract::We have previously shown for the paramyxovirus simian virus 5 (SV5) that a functional promoter for RNA replication requires proper spacing between two discontinuous elements: a 19-base segment at the 3' terminus (conserved region I [CRI]) and an 18-base internal region (CRII) that is contained within the coding region...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3993-3998.2001
更新日期:2001-04-01 00:00:00
abstract::The products of the A33R and A36R genes of vaccinia virus are incorporated into the membranes of intracellular enveloped virions (IEV). When extracts of cells that had been infected with vaccinia virus and labeled with H(3)(32)PO(4) were immunoprecipitated with antibodies against the A33R protein, two prominent bands ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.303-310.2001
更新日期:2001-01-01 00:00:00
abstract::Members of the Paramyxovirinae subfamily rely on the concerted action of two envelope glycoprotein complexes, attachment protein H and the fusion (F) protein oligomer, to achieve membrane fusion for viral entry. Despite advances in X-ray information, the organization of the physiological attachment (H) oligomer in fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01222-10
更新日期:2010-12-01 00:00:00
abstract::The Friend or Moloney mink cell focus-forming (MCF) virus encodes a recombinant-type envelope glycoprotein, gp70, that is closely related to the membrane glycoprotein, gp55, of Friend spleen focus-forming virus (SFFV). We have shown previously that gp55 has the ability to activate cell growth by binding to the cellula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2408-2414.1991
更新日期:1991-05-01 00:00:00
abstract::Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that has a worldwide distribution in the population of cattle. Many factors make human contamination by BoHV-4 likely to occur. In this study, we performed in vitro experiments to assess the risk and the consequences of human infection by BoHV-4. First, by using a re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2336-2347.2004
更新日期:2004-03-01 00:00:00
abstract::Utilization of internal ribosome entry segment (IRES) structures in the 5' noncoding region (5'NCR) of picornavirus RNAs for initiation of translation requires a number of host cell factors whose distribution may vary in different cells and whose requirement may vary for different picornaviruses. We have examined the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9668-9675.1998
更新日期:1998-12-01 00:00:00
abstract::Heat shock protein 70 (Hsp70) is incorporated within the membrane of primate lentiviral virions. Here we demonstrate that Hsp70 is also incorporated into oncoretroviral virions and that it remains associated with membrane-stripped human immunodeficiency virus type 1 (HIV-1) virion cores. To determine if Hsp70 promotes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5557-5567.2005
更新日期:2005-05-01 00:00:00
abstract::Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-16
更新日期:2017-03-29 00:00:00
abstract::T cells are central players in the immune response to infectious disease, with the specificity of their responses controlled by the T-cell receptor (TCR)/CD3 complex on the cell surface. Impairment of TCR/CD3-directed CD4(+) T-cell immune responses is frequently observed in individuals infected with human immunodefici...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.5207-5213.1999
更新日期:1999-06-01 00:00:00
abstract::The latent membrane protein 1 (LMP-1) of Epstein-Barr virus (EBV) functionally resembles a constitutively active, CD40-like receptor and contributes to the maintenance of proliferation of EBV-infected primary human B lymphocytes. LMP-1 is targeted to the plasma membrane, where it binds TRAF, TRADD, and JAK molecules t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.20.9755-9761.2000
更新日期:2000-10-01 00:00:00
abstract::Hepatitis C virus core protein forms the viral nucleocapsid and plays a critical role in the formation of infectious particles. In this study, we demonstrate that the highly conserved residue G33, located within domain 1 of the core protein, is important for the production of cell culture-infectious virus (HCVcc). Ala...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05452-11
更新日期:2012-01-01 00:00:00
abstract::Large numbers of polymorphonuclear leukocytes (PMNs) infiltrated the murine vaginal mucosa within 24 h after intravaginal inoculation with an attenuated strain of herpes simplex virus type 2 (HSV-2). The role of these cells in resolution of a primary genital infection and in protection of HSV-immune animals against ch...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6380-6386.1999
更新日期:1999-08-01 00:00:00
abstract::Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7598-7602.1998
更新日期:1998-09-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA virus, is an important causative agent of waterborne hepatitis. Expression of cDNA (encoding amino acids 1 to 979 of HEV nonstructural open reading frame 1) in insect cells resulted in synthesis of a 110-kDa protein (P110), a fraction of which was proteolytically processe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6249-6255.2001
更新日期:2001-07-01 00:00:00
abstract::Hepatitis delta virus (HDV) replicates by RNA-dependent RNA synthesis according to a double rolling circle model. Also synthesized during replication is a 0.8-kb, polyadenylated mRNA encoding the hepatitis delta antigen (HDAg). It has been proposed that this mRNA species represents the initial product of HDV RNA repli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5449-5456.1998
更新日期:1998-07-01 00:00:00
abstract::To determine the various roles of RNase H in reverse transcription, we generated a panel of mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase based on sequence alignments and the crystal structures of Escherichia coli and human immunodeficiency virus type 1 RNases H (S. W. Blain an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4440-4452.1995
更新日期:1995-07-01 00:00:00
abstract::An in vitro method which led to the transformation of hamster embryo fibroblasts after exposure to herpes simplex virus type 2 (HSV-2) inactivated with ultraviolet irradiation is described. The transformed cells (333-8-9) produced tumors when inoculated into newborn Syrian hamsters but not when injected into weanling ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.4.469-477.1971
更新日期:1971-10-01 00:00:00
abstract::Molecular clones of the subgroup A feline leukemia virus FeLV-A/Glasgow-1 have been obtained. Nucleotide sequence analysis of the 3' end of the proviral genome and comparison with the published sequence of FeLV-B/Gardner-Arnstein showed that the most extensive differences are located within the 5' domain of the env ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.825-834.1986
更新日期:1986-06-01 00:00:00
abstract::Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) were examined by using polyclonal rabbit antibodies and monoclonal antibodies against HHV-6-infected cells and human sera. More than 20 proteins and six glycoproteins specific for HHV-6-infected cells were identified from [35S]methionine-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2835-2840.1989
更新日期:1989-06-01 00:00:00
abstract::The enhancer unit present in the retrovirus equine infectious anemia virus (EIAV) was previously shown to contain binding sites for proteins belonging to MDBP, PEA2, AP-1, and ets families. The EIAV ets motif matches the consensus sequence for both PEA3- and PU.1-binding sites. Here, we show by gel shift analysis that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3885-3890.1993
更新日期:1993-07-01 00:00:00
abstract::The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01051-08
更新日期:2008-10-01 00:00:00