Abstract:
:The latent membrane protein 1 (LMP-1) of Epstein-Barr virus (EBV) functionally resembles a constitutively active, CD40-like receptor and contributes to the maintenance of proliferation of EBV-infected primary human B lymphocytes. LMP-1 is targeted to the plasma membrane, where it binds TRAF, TRADD, and JAK molecules to activate NF-kappaB-, AP-1-, and STAT-dependent pathways as does CD40. Yet LMP-1 appears to lack a ligand to regulate its signaling. We have found that LMP-1, when expressed at physiologic levels, inhibits gene expression detectably. Higher levels of LMP-1 expression eventually inhibit both the steady-state level of RNA produced from a BamHI C promoter reporter and general cellular protein synthesis. These findings indicate that LMP-1 can limit its signaling and that this control is manifest at two levels. The domain of LMP-1 that binds TRAF, TRADD, and JAK/STAT molecules is not required for this regulation. A derivative of LMP-1 that contains only its amino-terminal and membrane-spanning domains is sufficient to inhibit reporter activity when the reporter genes are expressed from the BamHI C and LMP-1 promoters. This same derivative of LMP-1 in parallel assays is sufficient to inhibit wild-type LMP-1's stimulation of NF-kappaB-dependent gene expression. We suggest that LMP-1 encodes stimulatory and inhibitory activities; the latter could limit signaling in the apparent absence of ligand-dependent down-regulation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sandberg ML,Kaykas A,Sugden Bdoi
10.1128/jvi.74.20.9755-9761.2000subject
Has Abstractpub_date
2000-10-01 00:00:00pages
9755-61issue
20eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
杂志文章abstract::Cell-to-cell movement of beet yellows closterovirus requires four structural proteins and a 6-kDa protein (p6) that is a conventional, nonstructural movement protein. Here we demonstrate that either virus infection or p6 overexpression results in association of p6 with the rough endoplasmic reticulum. The p6 protein p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3704-3709.2004
更新日期:2004-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is a prevalent pathogen that establishes lifelong infection in the host. Virus persistence is aided by extensive manipulation of the host immune system, particularly cytokine and chemokine signaling pathways. The HCMV UL111A gene encodes cmvIL-10, an ortholog of human interleukin-10 that h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01981-17
更新日期:2018-02-12 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) infection in the central nervous system (CNS) is characterized by replication in macrophages or brain microglia that express low levels of the CD4 receptor and is the cause of HIV-associated dementia and related cognitive and motor disorders that affect 20 to 30% o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01237-14
更新日期:2014-08-01 00:00:00
abstract::Natural Killer (NK) cells are important in the immune response to a number of viruses; however, the mechanisms used by NK cells to discriminate between healthy and virus-infected cells are only beginning to be understood. Infection with vaccinia virus provokes a marked increase in the susceptibility of target cells to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2225-2233.2006
更新日期:2006-03-01 00:00:00
abstract::We have examined the influence of the V1/V2 region of the human immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic properties of the virus. We observed that on the genomic background of the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of the macrophage-tropic strain, HIV-1SF162mc, in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2253-2259.1994
更新日期:1994-04-01 00:00:00
abstract::We report that adaptation to infect the guinea pig did not modify the capacity of foot-and-mouth disease virus (FMDV) to kill suckling mice and to cause an acute and transmissible disease in the pig, an important natural host for this pathogen. Adaptive amino acid replacements (I(248)-->T in 2C, Q(44)-->R in 3A, and L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00340-07
更新日期:2007-08-01 00:00:00
abstract::The large scale of the Ebola virus disease (EVD) outbreak in West Africa in 2013-2016 raised the question whether the host cell interactions of the responsible Ebola virus (EBOV) strain differed from those of other ebolaviruses. We previously reported that the glycoprotein (GP) of the virus circulating in West Africa ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00177-17
更新日期:2017-04-13 00:00:00
abstract::We have used deletion and recombinant long terminal repeat (LTR) mutants to examine enhancer activity differences between LTRs of the nonpathogenic Akv and the thymus lymphomagenic MCF13 murine retroviruses. Deletion mutant analysis revealed that major control regions for MCF13 and Akv LTR enhancer activity were simil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3353-3361.1989
更新日期:1989-08-01 00:00:00
abstract::The vif gene of human immunodeficiency virus type 1 is absolutely required for productive infection of primary cells derived from human blood and certain immortalized T lymphoid cells, for example, H9. Cells with this restrictive phenotype are termed nonpermissive, whereas cell lines in which vif-deficient virus can r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5297-5305.1996
更新日期:1996-08-01 00:00:00
abstract::The papillomavirus E2 gene product plays a pivotal role in viral replication. E2 has multiple functions, including (i) transcriptional activation and repression of viral promoters and (ii) the enhancement of viral DNA replication. It was previously reported that E2 suppressed the growth of papillomavirus-positive cerv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3752-3760.2000
更新日期:2000-04-01 00:00:00
abstract::Induction of virus-specific T-cell responses in mucosal as well as systemic compartments of the immune system is likely to be a critical feature of an effective AIDS vaccine. We investigated whether virus-specific CD8(+) lymphocytes induced in rhesus macaques by immunization with attenuated simian immunodeficiency vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8762-8766.2000
更新日期:2000-09-01 00:00:00
abstract::The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) can be proteolytically activated by cathepsins B and L upon viral uptake into target cell endosomes. In contrast, it is largely unknown whether host cell proteases located in the secretory pathway of infected cells and/or on the surf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02232-10
更新日期:2011-05-01 00:00:00
abstract::Genomic variation and related evolutionary dynamics of human respiratory syncytial virus (RSV), a common causative agent of severe lower respiratory tract infections, may affect its transmission behavior. RSV evolutionary patterns are likely to be influenced by a precarious interplay between selection favoring variant...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03278-12
更新日期:2013-07-01 00:00:00
abstract::We have analyzed the effects of reducing conditions on the folding of the spike (S) protein and on the intracellular transport of the membrane (M) protein of the mouse hepatitis coronavirus. These proteins differ in their potential to form disulfide bonds in the lumen of the endoplasmic reticulum (ER). Intrachain disu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7394-7401.1993
更新日期:1993-12-01 00:00:00
abstract::Three double-stranded RNA segments of bacteriophage phi 6 (L, M, and S) were transcribed in vitro by a virion-associated RNA polymerase. Regulation of L transcription was distinct from regulation of M and S transcription. Transcription of the L segment, which codes for early proteins, required manganous ion and high c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.196-203.1983
更新日期:1983-04-01 00:00:00
abstract::Human hepatitis B virus (HBV) is a small DNA virus that replicates inside the viral nucleocapsid by reverse transcription of an RNA intermediate. Encapsidation of this RNA pregenome is mediated by the interaction of the viral replication enzyme P with the structured 5'-proximal RNA element epsilon; replication was tho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.2764-2773.1996
更新日期:1996-05-01 00:00:00
abstract::We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05624-11
更新日期:2011-12-01 00:00:00
abstract::Region 1 DNA sequences (map positions 0 to 11% on the linear adenovirus 2 genome) are expressed both early and late in lytic infection and are required for transformation by the virus. During productive infection six distinct cytoplasmic RNAs are synthesized from this region. These RNAs comprise two families, each con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.621-629.1979
更新日期:1979-09-01 00:00:00
abstract::Monoclonal antibodies (MAbs) directed against epitopes in the V2 domain of human immunodeficiency virus type 1 gp120 often possess neutralizing activity, but these generally are highly type specific, neutralize only laboratory isolates, or have low potency. The most potent of these is C108g, directed against a type-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.6909-6917.2005
更新日期:2005-06-01 00:00:00
abstract::Ten bacteriophages that attack and lyse saprophytic strains of Bdellovibrio bacteriovorus were isolated. Morphological, serological, and host-range studies revealed that there were four different bdellovibrio phages present among the isolates. One of the phages lysed a strain of B. bacteriovorus that requires the pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.3.516-523.1972
更新日期:1972-09-01 00:00:00
abstract::Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.2.480-488.1977
更新日期:1977-05-01 00:00:00
abstract::We sequenced the genes coding for the two neutralization proteins, VP4 and VP7, of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5640-5644.1990
更新日期:1990-11-01 00:00:00
abstract::HIV-1 infections cannot be completely eradicated by drug therapy, as the virus persists in reservoirs. Low-level plasma viremia has been detected in patients treated for over 7 years, but the cellular compartments that support this low-level viremia have not been identified. The decay of HIV-1 during treatment appears...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00556-13
更新日期:2013-07-01 00:00:00
abstract:UNLABELLED:The latent HIV reservoir is a major impediment to curing HIV infection. The contribution of CD4(+) T cell activation status to the establishment and maintenance of the latent reservoir was investigated by enumerating viral DNA components in a cohort of 12 individuals commencing antiretroviral therapy (ART) c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03331-13
更新日期:2014-03-01 00:00:00
abstract::The efficiency of processing of polyoma viral RNA and of its export from nucleus to cytoplasm was measured in primary mouse kidney cells by comparing the initial rates of incorporation of [3H]uridine into cytoplasmic and nuclear viral RNA. Appropriate methods of cell fractionation were chosen to maximize yields of cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.628-635.1981
更新日期:1981-02-01 00:00:00
abstract::Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3512-3526.1994
更新日期:1994-06-01 00:00:00
abstract::Humans chronically infected with hepatitis B virus (HBV) are at further risk of liver cancer upon exposure to dietary aflatoxin B1 (AFB1), a carcinogenic product of the mold Aspergillus flavus. For the present study, we utilized double-transgenic mice (ATX mice) that express the HBV X protein (HBx) and possess a bacte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11770-11774.2002
更新日期:2002-11-01 00:00:00
abstract::The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters em...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01259-13
更新日期:2013-09-01 00:00:00
abstract::Some animal rotaviruses require the presence of sialic acid (SA) on the cell surface to infect the cell. We have isolated variants of rhesus rotavirus (RRV) whose infectivity no longer depends on SA. Both the SA-dependent and -independent interactions of these viruses with the cell are mediated by the virus spike prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.593-599.2000
更新日期:2000-01-01 00:00:00
abstract::The intranuclear structure of parental adenovirus 2 DNA was studied using digestion with micrococcal nuclease as a probe. When cultures were infected with 32P-labeled virions, at a multiplicity of 3,000 particles per cell, 14 to 21% of parental DNA penetrated the cell and reached the nucleus. Of this parental DNA, 60%...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.888-898.1979
更新日期:1979-03-01 00:00:00