The comparative genomics of human respiratory syncytial virus subgroups A and B: genetic variability and molecular evolutionary dynamics.


:Genomic variation and related evolutionary dynamics of human respiratory syncytial virus (RSV), a common causative agent of severe lower respiratory tract infections, may affect its transmission behavior. RSV evolutionary patterns are likely to be influenced by a precarious interplay between selection favoring variants with higher replicative fitness and variants that evade host immune responses. Studying RSV genetic variation can reveal both the genes and the individual codons within these genes that are most crucial for RSV survival. In this study, we conducted genetic diversity and evolutionary rate analyses on 36 RSV subgroup B (RSV-B) whole-genome sequences. The attachment protein, G, was the most variable protein; accordingly, the G gene had a higher substitution rate than other RSV-B genes. Overall, less genetic variability was found among the available RSV-B genome sequences than among RSV-A genome sequences in a comparable sample. The mean substitution rates of the two subgroups were, however, similar (for subgroup A, 6.47 × 10(-4) substitutions/site/year [95% credible interval {CI 95%}, 5.56 × 10(-4) to 7.38 × 10(-4)]; for subgroup B, 7.76 × 10(-4) substitutions/site/year [CI 95%, 6.89 × 10(-4) to 8.58 × 10(-4)]), with the time to their most recent common ancestors (TMRCAs) being much lower for RSV-B (19 years) than for RSV-A (46.8 years). The more recent RSV-B TMRCA is apparently the result of a genetic bottleneck that, over longer time scales, is still compatible with neutral population dynamics. Whereas the immunogenic G protein seems to require high substitution rates to ensure immune evasion, strong purifying selection in conserved proteins such as the fusion protein and nucleocapsid protein is likely essential to preserve RSV viability.


J Virol


Journal of virology


Tan L,Coenjaerts FE,Houspie L,Viveen MC,van Bleek GM,Wiertz EJ,Martin DP,Lemey P




Has Abstract


2013-07-01 00:00:00














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    journal_title:Journal of virology

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    authors: Wiesmüller L,Cammenga J,Deppert WW

    更新日期:1996-02-01 00:00:00

  • Relative replication fitness of a high-level 3'-azido-3'-deoxythymidine-resistant variant of human immunodeficiency virus type 1 possessing an amino acid deletion at codon 67 and a novel substitution (Thr-->Gly) at codon 69.

    abstract::The combination of an amino acid deletion at codon 67 (delta 67) and Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is associated with high-level resistance to multiple RT inhibitors. To determine the relative contributions of the delta 67 and T69G...

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    authors: Imamichi T,Berg SC,Imamichi H,Lopez JC,Metcalf JA,Falloon J,Lane HC

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  • Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.

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    authors: Chen SC,Jeng KS,Lai MMC

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  • Adenovirus type 12 gene 401 function and maintenance of transformation.

    abstract::Rat (3Y1) and hamster embryo brain cells were transformed by wild-type adenovirus type 12 or the DNA-minus temperature-sensitive mutant ts401. The ts401-transformed 3Y1 cells, but not the wild-type transformants, displayed a temperature-sensitive response with respect to the following characteristics of the transforme...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ledinko N,Schaeufele J,Soorma O

    更新日期:1979-01-01 00:00:00

  • The amphibian (Xenopus laevis) type I interferon response to frog virus 3: new insight into ranavirus pathogenicity.

    abstract:UNLABELLED:The increasing prevalence of ranavirus (RV; Iridoviridae) infections of wild and commercially maintained aquatic species is raising considerable concerns. While Xenopus laevis is the leading model for studies of immunity to RV, amphibian antiviral interferon (IFN) responses remain largely uncharacterized. Ac...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Grayfer L,De Jesús Andino F,Robert J

    更新日期:2014-05-01 00:00:00

  • N-linked glycan at residue 523 of human parainfluenza virus type 3 hemagglutinin-neuraminidase masks a second receptor-binding site.

    abstract::The hemagglutinin-neuraminidase (HN) glycoprotein plays a critical role in parainfluenza virus replication. We recently found that in addition to the catalytic binding site, HN of human parainfluenza virus type 1 (hPIV-1) may have a second receptor-binding site covered by an N-linked glycan at residue 173, which is ne...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mishin VP,Watanabe M,Taylor G,Devincenzo J,Bose M,Portner A,Alymova IV

    更新日期:2010-03-01 00:00:00

  • In vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms.

    abstract::NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Poncet D,Lindenbaum P,L'Haridon R,Cohen J

    更新日期:1997-01-01 00:00:00

  • Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Inhibits Major Histocompatibility Complex Class II Expression by Disrupting Enhanceosome Assembly through Binding with the Regulatory Factor X Complex.

    abstract:UNLABELLED:Major histocompatibility complex class II (MHC-II) molecules play a central role in adaptive antiviral immunity by presenting viral peptides to CD4(+) T cells. Due to their key role in adaptive immunity, many viruses, including Kaposi's sarcoma-associated herpesvirus (KSHV), have evolved multiple strategies ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Thakker S,Purushothaman P,Gupta N,Challa S,Cai Q,Verma SC

    更新日期:2015-05-01 00:00:00

  • Adenovirus Core Protein VII Downregulates the DNA Damage Response on the Host Genome.

    abstract::Viral manipulation of cellular proteins allows viruses to suppress host defenses and generate infectious progeny. Due to the linear double-stranded DNA nature of the adenovirus genome, the cellular DNA damage response (DDR) is considered a barrier to successful infection. The adenovirus genome is packaged with protein...

    journal_title:Journal of virology

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    authors: Avgousti DC,Della Fera AN,Otter CJ,Herrmann C,Pancholi NJ,Weitzman MD

    更新日期:2017-09-27 00:00:00

  • Nucleotide composition of the ribonucleic acid of rabies virus.

    abstract::The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Aaslestad HG,Urbano C

    更新日期:1971-12-01 00:00:00

  • Tetramerization of Phosphoprotein is Essential for Respiratory Syncytial Virus Budding while its N Terminal Region Mediates Direct Interactions with the Matrix Protein.

    abstract::It was shown previously that the Matrix (M), Phosphoprotein (P), and the Fusion (F) proteins of Respiratory syncytial virus (RSV) are sufficient to produce virus-like particles (VLPs) that resemble the RSV infection-induced virions. However, the exact mechanism and interactions among the three proteins are not known. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bajorek M,Galloux M,Richard CA,Szekely O,Rosenzweig R,Sizun C,Eleouet JF

    更新日期:2021-01-06 00:00:00

  • Genomic analysis reveals age-dependent innate immune responses to severe acute respiratory syndrome coronavirus.

    abstract::The relationship between immunosenescence and the host response to virus infection is poorly understood at the molecular level. Two different patterns of pulmonary host responses to virus were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Baas T,Roberts A,Teal TH,Vogel L,Chen J,Tumpey TM,Katze MG,Subbarao K

    更新日期:2008-10-01 00:00:00

  • Restriction endonuclease mapping of unintegrated viral DNA of B- and N-tropic BALB/c murine leukemia virus.

    abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rassart E,Jolicoeur P

    更新日期:1980-09-01 00:00:00

  • Strain variation of R5 direct repeats in the right-hand portion of the long unique segment of varicella-zoster virus DNA.

    abstract::We located a region of interstrain size variability in a short segment in an area at the right-hand end of the long unique sequence of the varicella-zoster viral genome. Varicella-zoster virus strains isolated in a district of Japan were classified into three groups on the basis of the size of this segment. Sequence c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hondo R,Yogo Y

    更新日期:1988-08-01 00:00:00

  • Breaking the Convention: Sialoglycan Variants, Coreceptors, and Alternative Receptors for Influenza A Virus Entry.

    abstract::The influenza A virus (IAV) envelope protein hemagglutinin binds α2,6- or α2,3-linked sialic acid as a host cell receptor. Bat IAV subtypes H17N10 and H18N11 form an exception to this rule and do not bind sialic acid but enter cells via major histocompatibility complex (MHC) class II. Here, we review current knowledge...

    journal_title:Journal of virology

    pub_type: 杂志文章,评审


    authors: Karakus U,Pohl MO,Stertz S

    更新日期:2020-01-31 00:00:00

  • A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression.

    abstract::We have recovered a virion from defective cDNA of Sendai virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene expression vector derived from a defective viral genome o...

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    authors: Li HO,Zhu YF,Asakawa M,Kuma H,Hirata T,Ueda Y,Lee YS,Fukumura M,Iida A,Kato A,Nagai Y,Hasegawa M

    更新日期:2000-07-01 00:00:00

  • The human interferon-induced MxA protein inhibits early stages of influenza A virus infection by retaining the incoming viral genome in the cytoplasm.

    abstract::The induction of an interferon-induced antiviral state is a powerful cellular response against viral infection that limits viral spread. Here, we show that a preexisting antiviral state inhibits the replication of influenza A viruses in human A549 cells by preventing transport of the viral genome to the nucleus and th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Xiao H,Killip MJ,Staeheli P,Randall RE,Jackson D

    更新日期:2013-12-01 00:00:00

  • Fusion of the termini of the murine cytomegalovirus genome after infection.

    abstract::The genome of murine cytomegalovirus, extracted from extracellular virions, is a linear double-stranded DNA molecule ca. 240 kilobase pairs long. In our initial cloning of subgenomic fragments of the murine cytomegalovirus genome, we obtained a HindIII clone which contained fused HindIII-terminal fragments. By hybridi...

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    pub_type: 杂志文章


    authors: Marks JR,Spector DH

    更新日期:1984-10-01 00:00:00

  • Neuroadapted yellow fever virus 17D: determinants in the envelope protein govern neuroinvasiveness for SCID mice.

    abstract::A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...

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    pub_type: 杂志文章


    authors: Nickells M,Chambers TJ

    更新日期:2003-11-01 00:00:00

  • TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core.

    abstract::TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to t...

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    authors: Valle-Casuso JC,Di Nunzio F,Yang Y,Reszka N,Lienlaf M,Arhel N,Perez P,Brass AL,Diaz-Griffero F

    更新日期:2012-05-01 00:00:00

  • Modification of human papillomavirus minor capsid protein L2 by sumoylation.

    abstract::The human papillomavirus (HPV) minor capsid protein L2 plays important roles in the generation of infectious viral particles and in the initial steps of infection. Here we show that HPV-16 L2 protein is sumoylated at lysine 35 and that sumoylation affects its stability. Interestingly, the sumoylated form of L2 cannot ...

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    authors: Marusic MB,Mencin N,Licen M,Banks L,Grm HS

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  • Biological and biochemical characteristics of prion strains conserved in persistently infected cell cultures.

    abstract::Abnormal prion protein (PrP(Sc)) plays a central role in the transmission of prion diseases, but the molecular basis of prion strains with distinct biological characteristics remains to be elucidated. We analyzed the characteristics of prion disease by using mice inoculated with the Chandler and Fukuoka-1 strains prop...

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    authors: Arima K,Nishida N,Sakaguchi S,Shigematsu K,Atarashi R,Yamaguchi N,Yoshikawa D,Yoon J,Watanabe K,Kobayashi N,Mouillet-Richard S,Lehmann S,Katamine S

    更新日期:2005-06-01 00:00:00

  • Viral sanctuaries during highly active antiretroviral therapy in a nonhuman primate model for AIDS.

    abstract::Highly active antiretroviral therapy (HAART) enables long-term suppression of plasma HIV-1 loads in infected persons, but low-level virus persists and rebounds following cessation of therapy. During HAART, this virus resides in latently infected cells, such as resting CD4(+) T cells, and in other cell types that may s...

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    authors: North TW,Higgins J,Deere JD,Hayes TL,Villalobos A,Adamson L,Shacklett BL,Schinazi RF,Luciw PA

    更新日期:2010-03-01 00:00:00

  • General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.

    abstract::The production and selection of infectious vaccinia virus recombinants expressing foreign genes was facilitated by the construction of plasmid vectors. These vectors contain all or part of the vaccinia virus thymidine kinase (TK) gene interrupted by multiple unique restriction endonuclease sites placed adjacent to the...

    journal_title:Journal of virology

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    authors: Mackett M,Smith GL,Moss B

    更新日期:1984-03-01 00:00:00

  • Cultivation and characterization of three strains of murine rotavirus.

    abstract::Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses...

    journal_title:Journal of virology

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    authors: Greenberg HB,Vo PT,Jones R

    更新日期:1986-02-01 00:00:00

  • Transient association of calnexin and calreticulin with newly synthesized G1 and G2 glycoproteins of uukuniemi virus (family Bunyaviridae).

    abstract::The membrane glycoproteins G1 and G2 of Uukuniemi virus, a member of the Bunyaviridae family, are cotranslationally cleaved from a common precursor in the endoplasmic reticulum (ER). Here, we show that newly made G1 and G2 associate transiently with calnexin and calreticulin, two lectins involved in glycoprotein foldi...

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    authors: Veijola J,Pettersson RF

    更新日期:1999-07-01 00:00:00

  • Early bone marrow hematopoietic defect in simian/human immunodeficiency virus C2/1-infected macaques and relevance to advance of disease.

    abstract::To clarify hematological abnormalities following infection with human immunodeficiency virus (HIV), we examined the hematopoietic capability of bone marrow by using cynomolgus monkeys infected with pathogenic simian/human immunodeficiency virus (SHIV) strain C2/1, an animal model of HIV infection. The relationship bet...

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    authors: Yamakami K,Honda M,Takei M,Ami Y,Kitamura N,Nishinarita S,Sawada S,Horie T

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  • Structure of varicella-zoster virus DNA.

    abstract::Varicella-zoster virus (VZV) DNA was prepared from nucleocapsids and from enveloped virions of a laboratory strain (Ellen) and directly from the vesicle fluids of patients with zoster infections. VZV Ellen nucleocapsid DNA was cleaved with 11 different restriction endonucleases and electrophoresed in agarose gels. The...

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    pub_type: 杂志文章


    authors: Straus SE,Aulakh HS,Ruyechan WT,Hay J,Casey TA,Vande Woude GF,Owens J,Smith HA

    更新日期:1981-11-01 00:00:00

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    abstract::Reoviruses are encapsidated double-stranded RNA viruses that cause systemic disease in mice after peroral (p.o.) inoculation and primary replication in the intestine. In this study, we define components of the immune system involved in the clearing of reovirus from the proximal small intestine. The intestines of immun...

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    pub_type: 杂志文章


    authors: Barkon ML,Haller BL,Virgin HW 4th

    更新日期:1996-02-01 00:00:00

  • High-risk human papillomavirus E6 oncoproteins interact with 14-3-3ζ in a PDZ binding motif-dependent manner.

    abstract::Cervical cancer develops through the combined activities of the human papillomavirus (HPV) E6 and E7 oncoproteins. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 prote...

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    authors: Boon SS,Banks L

    更新日期:2013-02-01 00:00:00