Abstract:
:After incubation at 37 C for 2 or more days, Basal Medium Eagle (BME) supplemented with 25% fetal calf serum (FCS) was not able to support adequate growth of EB3 and other lines of Burkitt tumor cells. The medium did, however, increase, by a factor of about 10, the number of cells synthesizing herpes-type virus with which the cultures were persistently infected. Not every lot of FCS produced these effects, nor were these effects seen when BME and FCS were incubated separately for 7 days before the medium was completed. At 37 C, appropriate lots of FCS interacted with several of the amino acids present in BME; this interaction resulted in an inhibition of cellular growth, whereas interaction with arginine yielded the virus-enhancing effect. Arginine-free BME, supplemented with 25% FCS and used without prior incubation, prevented cellular replication and promoted viral infection to a similar extent as did preincubated complete medium. Replenishment of arginine reduced, but did not regularly abolish, the virus-enhancing activity of preincubated media. RPMI-1629 medium was less affected by preincubation with FCS because it contained twice the amount of arginine that BME contained. The FCS factors which act upon arginine and other amino acids are not dialyzable and are partially resistant to heating at 56 or 60 C for 30 to 60 min. Calf and horse sera appear to be devoid of these activities. The nature of these interactions, as well as the mechanism by which arginine deficiency enhanced the viral infection, remains to be ascertained.
journal_name
J Viroljournal_title
Journal of virologyauthors
Henle W,Henle Gdoi
10.1128/JVI.2.3.182-191.1968subject
Has Abstractpub_date
1968-03-01 00:00:00pages
182-91issue
3eissn
0022-538Xissn
1098-5514journal_volume
2pub_type
杂志文章abstract::To determine whether the tripartite leader is required for efficient translation in adenovirus-infected cells at late times of infection, we constructed recombinant adenoviruses containing the influenza virus nucleocapsid protein (NP) gene expressed under the control of the adenovirus major late promoter (MLP). We cho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1606-1616.1988
更新日期:1988-05-01 00:00:00
abstract::Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a glutamine codon (CAG) at the junction did not replicate. A revertant of this virus consisted of the original virus and a virus w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6376-6379.1991
更新日期:1991-11-01 00:00:00
abstract::Primary RNA transcripts from the human immunodeficiency virus type 1 (HIV-1) are processed into mature mRNA by a complex series of splicing events. Viral structural proteins and reverse transcriptase are translated from unspliced or singly spliced transcripts. Proteins which control virus replication, including tat, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3391-3398.1990
更新日期:1990-07-01 00:00:00
abstract::Using a method for freeze-drying intact cells, uninfected and murine leukemia virus (MuLV)-infected JLSV9 cell surfaces, as well as murine mammary tumor virus (MuMTV)-infected cell surfaces, were examined by electron microscopy. The 10-nm knobs of MuLV and the 5-nm spikes of MuMTV were clearly revealed on the surfaces...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.358-365.1977
更新日期:1977-01-01 00:00:00
abstract::We have cloned several prototypic members of the family of human endogenous retroviruslike elements having a histidine tRNA primer-binding site (RTVL-H) and have determined the nucleotide sequence of one of these clones (RTVL-H2). The RTVL-H2 sequence is 5,813 nucleotides long, with long terminal repeats of 450 nucleo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4060-4066.1987
更新日期:1987-12-01 00:00:00
abstract::An immunological hierarchy among three H-2Db-restricted cytotoxic T lymphocyte (CTL) determinants in simian virus 40 (SV40) large T antigen (Tag) was described previously: determinants I and II/III are immunodominant, whereas determinant V is immunorecessive. To assess the immunogenicity of each determinant individual...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1469-1481.1998
更新日期:1998-02-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infection causes the active degradation of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), and this process is reliant on the expression of the HSV-1 immediate-early protein Vmw110. In this study we investigated in more detail the mechanism by which the degradation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.650-657.1999
更新日期:1999-01-01 00:00:00
abstract::Sensory neurons within trigeminal ganglia (TG) are the primary site for bovine herpesvirus 1 (BHV-1) latency. During latency, viral gene expression is restricted to the latency-related (LR) gene and the open reading frame ORF-E. We previously constructed an LR mutant virus that expresses LR RNA but not any of the know...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01009-09
更新日期:2009-09-01 00:00:00
abstract::The DNAs of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses contain overlapping segments of the early region of wild-type SV40 DNA. The complementary DNA strands of these five viruses have been separated with synthetic polyribonucleotides in isopycnic cesium chloride gradients. The relat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.3.677-689.1974
更新日期:1974-03-01 00:00:00
abstract::The techniques of deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization and immunological precipitation were used to compare the synthesis of adenovirus-specific macromolecules in African green monkey kidney (AGMK) cells infected with adenovirus, an abortive infection, and coinfected with both adenovirus and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1109-1117.1972
更新日期:1972-12-01 00:00:00
abstract::The combination of an amino acid deletion at codon 67 (delta 67) and Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is associated with high-level resistance to multiple RT inhibitors. To determine the relative contributions of the delta 67 and T69G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.10958-10964.2000
更新日期:2000-12-01 00:00:00
abstract::Storage of the dimeric (alphabeta) form of avian myeloblastosis virus (AMV) DNA polymerase in glycerol resulted in the release of the smaller alpha subunit, as detected by glycerol gradient sedimentation. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of enzyme stored in glycerol showed the conc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.904-910.1976
更新日期:1976-06-01 00:00:00
abstract::Two strains of minute virus of mice (MVM) show different host cell specificities. The prototype strain MVM(p) grows in fibroblasts, whereas the immunosuppressive variant MVM(i) grows in T lymphocytes. In this study, we have mapped on the viral genome a cell type-specific determinant: it is located between 69 and 85 ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.552-557.1988
更新日期:1988-02-01 00:00:00
abstract::The entire measles virus (MV) hemagglutinin (HA)-coding region was reconstructed from cloned cDNAs and used as part of a hybrid transcription unit to replace a region of the adenovirus type 5 genome corresponding to the entire E1a transcription unit and most of the E1b transcription unit. The resulting recombinant vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2718-2727.1988
更新日期:1988-08-01 00:00:00
abstract::Evidence is presented that the herpes simplex virus type 2 glycoprotein previously designated gF is antigenically related to herpes simplex virus type 1 gC (gC-1). An antiserum prepared against type 1 virion envelope proteins immunoprecipitated gF of type 2 (gF-2), and competition experiments revealed that the anti-gC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.553-562.1983
更新日期:1983-09-01 00:00:00
abstract::Recently, it was demonstrated that the coxsackievirus B3 variant PD (CVB3 PD) is able to infect coxsackievirus-adenovirus receptor (CAR)-lacking cells by using heparan sulfates (HS) as additional receptors (A. E. Zautner, U. Korner, A. Henke, C. Badorff, and M. Schmidtke, J. Virol. 77:10071-10077, 2003). For this stud...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01988-05
更新日期:2006-07-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::Infectious bursal disease virus (IBDV), a double-stranded RNA (dsRNA) virus belonging to the Birnaviridae family, is an economically important avian pathogen. The IBDV capsid is based on a single-shelled T=13 lattice, and the only structural subunits are VP2 trimers. During capsid assembly, VP2 is synthesized as a pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-07
更新日期:2007-07-01 00:00:00
abstract::West Nile virus (WNV) is a neurotropic mosquito-borne flavivirus of global importance. Neuroinvasive WNV infection results in encephalitis and can lead to prolonged neurological impairment or death. Type I interferon (IFN-I) is crucial for promoting antiviral defenses through the induction of antiviral effectors, whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01778-18
更新日期:2019-03-05 00:00:00
abstract::Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.464-467.1991
更新日期:1991-01-01 00:00:00
abstract::Wide application of the gene-targeting technique has been hampered by its low level of efficiency. A replication-defective adenovirus vector was used for efficient delivery of donor DNA in order to bypass this problem. Homologous recombination was selected between a donor neo gene inserted in the adenovirus vector and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6180-6190.1995
更新日期:1995-10-01 00:00:00
abstract::All known DNA tumor viruses are known to target and inactivate two main cell cycle regulatory proteins, retinoblastoma protein (pRb) and p53. Inactivation of pRb promotes host cell cycle progression into S phase, and inactivation of p53 promotes cell immortalization. The DNA tumor virus Kaposi's sarcoma associated her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10336-10347.2004
更新日期:2004-10-01 00:00:00
abstract:UNLABELLED:CBFβ was recently found to be a key regulator of the ability of human immunodeficiency virus type 1 (HIV-1) Vif to overcome host antiviral APOBEC3 proteins. However, the detailed molecular requirements for the Vif-CBFβ interaction are still not clear. Here, we mapped the minimum Vif domain required for CBFβ ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03604-13
更新日期:2014-03-01 00:00:00
abstract::Disruption of Epstein-Barr virus (EBV) latency is mediated by ZEBRA, the protein product of the immediate-early EBV gene, BZLF1. In vitro, phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), induces reactivation of EBV. However, the physiological stimuli responsible for the disruption ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5810-5818.2000
更新日期:2000-07-01 00:00:00
abstract::Arginine methylation of human immunodeficiency virus type 1 (HIV-1) Tat protein downregulates its key function in viral-gene transactivation. The fate of methylated Tat is unknown, so it is unclear whether methylated Tat is degraded or persists in the cell for additional functions. Here we show that the arginine methy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00499-09
更新日期:2009-11-01 00:00:00
abstract::Although many human immunodeficiency virus type 1 (HIV-1)-infected persons are treated with multiple protease inhibitors in combination or in succession, mutation patterns of protease isolates from these persons have not been characterized. We collected and analyzed 2,244 subtype B HIV-1 isolates from 1,919 persons wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4836-4847.2003
更新日期:2003-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) does not replicate in murine cells. We investigated the basis of this block by infecting a murine NIH 3T3 reporter cell line that stably expressed human CD4, CCR5, and cyclin T1 and contained a transactivatable HIV-1 long terminal repeat (LTR)-green fluorescent protein (GFP)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3859-3870.2000
更新日期:2000-04-01 00:00:00
abstract::We analyzed wild mouse DNAs for the number and type of proviral genes related to the env sequences of various murine leukemia viruses (MuLVs). Only Mus species closely related to laboratory mice carried these retroviral sequences, and the different subclasses of viral env genes tended to be restricted to specific taxo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3082-3088.1987
更新日期:1987-10-01 00:00:00
abstract::The development of immunization strategies to protect against ocular infection with herpes simplex virus 1 (HSV-1) must address the issue of the effects of the strategy on the establishment of latency in the trigeminal ganglia (TG). It is the reactivation of this latent virus that can cause recurrent disease and corne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00913-14
更新日期:2014-07-01 00:00:00
abstract::Hepatitis C virus (HCV) is an enveloped positive-stranded RNA hepatotropic virus. HCV pseudoparticles infect liver-derived cells, supporting a model in which liver-specific molecules define HCV internalization. Three host cell molecules have been reported to be important entry factors or receptors for HCV internalizat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02286-07
更新日期:2008-05-01 00:00:00