Abstract:
:Wide application of the gene-targeting technique has been hampered by its low level of efficiency. A replication-defective adenovirus vector was used for efficient delivery of donor DNA in order to bypass this problem. Homologous recombination was selected between a donor neo gene inserted in the adenovirus vector and a target mutant neo gene on a nuclear papillomavirus plasmid. These recombinant adenoviruses allowed gene transfer to 100% of the treated cells without impairing their viability. Homologous recombinants were obtained at a level of frequency much higher than that obtained by electroporation or a calcium phosphate procedure. The structure of the recombinants was analyzed in detail after recovery in an Escherichia coli strain. All of the recombinants examined had experienced a precise correction of the mutant neo gene. Some of them had a nonhomologous rearrangement of their sequences as well. One type of nonhomologous recombination took place at the end of the donor-target homology. The vector adenovirus DNA was inserted into some of the products obtained at a high multiplicity of infection. The insertion was at the end of the donor-target homology with a concomitant insertion of a 10-bp-long filler sequence in one of the recombinants. The possible relationship between these rearrangements and the homologous recombination is discussed. These results demonstrate the applicability of adenovirus-mediated gene delivery in gene targeting and gene therapy.
journal_name
J Viroljournal_title
Journal of virologyauthors
Fujita A,Sakagami K,Kanegae Y,Saito I,Kobayashi Idoi
10.1128/JVI.69.10.6180-6190.1995subject
Has Abstractpub_date
1995-10-01 00:00:00pages
6180-90issue
10eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Severe complications of Zika virus (ZIKV) infection might be caused by inflammation, but how ZIKV induces proinflammatory cytokines is not understood. In this study, we show opposite regulatory effects of the ZIKV NS5 protein on interferon (IFN) signaling. Whereas ZIKV and its NS5 protein were potent suppressors of ty...
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更新日期:1980-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.4012-4018.1987
更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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更新日期:2005-02-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.62.8.2629-2635.1988
更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.63.11.4913-4918.1989
更新日期:1989-11-01 00:00:00
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更新日期:1988-09-01 00:00:00
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更新日期:1979-12-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7362-7366.1995
更新日期:1995-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02339-06
更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00