Abstract:
:The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced similar amounts of INV and formed similar-size primary plaques but that IHD-J produced 10 to 40 times more EEV and spread to distant cells much more efficiently than did WR. Nevertheless, cells infected with WR and IHD-J had similar amounts of CEV, indicating that wrapping and transport of WR virions were unimpaired. A WR mutant with a deletion in VP37, the major outer envelope protein, formed normal amounts of INV; however, the generation of CEV was blocked and plaque formation was inhibited. These results suggested that CEV is the form of virus that mediates cell-to-cell spread. Marker rescue experiments indicated that the differences in EEV production by WR and IHD-J were not due to sequence differences in VP37. The low amount of WR EEV could be attributed to retention of CEV on the cell membrane. In support of this hypothesis, mild treatment with trypsin released as much or more infectious virus from cells infected with WR as it did with cells infected with IHD-J. Most of the virus released by trypsin sedimented with the buoyant density of EEV. Also, addition of trypsin to cells following inoculation with WR led to a comet-shaped distribution of secondary plaques characteristic of IHD-J. These results demonstrated that the release of CEV from the cell surface was limiting for extracellular virus formation and affirmed the role of EEV in long-range spread.
journal_name
J Viroljournal_title
Journal of virologyauthors
Blasco R,Moss Bdoi
10.1128/JVI.66.7.4170-4179.1992subject
Has Abstractpub_date
1992-07-01 00:00:00pages
4170-9issue
7eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
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pub_type: 杂志文章
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doi:10.1128/jvi.76.13.6669-6677.2002
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journal_title:Journal of virology
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journal_title:Journal of virology
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doi:10.1128/JVI.34.3.615-626.1980
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journal_title:Journal of virology
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更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章
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