Abstract:
:Phage XP-12, which has complete substitution of the cytosine residues in its DNA with 5-methylcytosine residues, was shown to inhibit incorporation of uracil into host DNA and RNA during the latent period. This apparent inhibition of host macromolecular synthesis was not accompanied by extensive degradation of the host chromosome. Phage DNA synthesis in infected cells occurred at a faster rate than host DNA synthesis in analogous uninfected cells. However, phage DNA synthesis could not be accurately monitored by incorporation of [methyl-3H]thymidine into DNA because, soon after infection, there was a marked inhibition of utilization of exogenous thymidine for DNA synthesis. Phage infection conferred upon a thymine auxotrophic host the ability to synthesize thymine nucleotides for phage DNA synthesis. It is suggested that a phage-induced thymidylate synthetase activity is partially responsible for the inhibition of thymidine incorporation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ehrlich M,Lin FH,Ehrlich K,Brown SL,Mayo JAdoi
10.1128/JVI.23.3.517-523.1977subject
Has Abstractpub_date
1977-09-01 00:00:00pages
517-23issue
3eissn
0022-538Xissn
1098-5514journal_volume
23pub_type
杂志文章abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-15
更新日期:2015-07-01 00:00:00
abstract::Transgene expression after the administration of recombinant adenovirus with E1 deleted is constantly transient. It is admitted that E1A-substituting activities of cellular or viral origin allow viral antigen synthesis and trigger cytotoxic lymphocyte-mediated clearance of the recipient cells. Our approach to solving ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.559-565.1996
更新日期:1996-01-01 00:00:00
abstract::In most human immunodeficiency virus type 1 (HIV-1)-infected individuals who achieve viral loads of <50 copies/ml during highly active antiretroviral therapy (HAART), low levels of plasma virus remain detectable for years by ultrasensitive methods. The relative contributions of ongoing virus replication and virus prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-09
更新日期:2009-10-01 00:00:00
abstract::We have used feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human immunodeficiency virus type 1 (HIV-1) PR. Our previous mutagenesis studies demonstrated that discrete substitutions in the acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00374-06
更新日期:2006-08-01 00:00:00
abstract::Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5886-5896.1998
更新日期:1998-07-01 00:00:00
abstract::During primary infection, the number of HIV-1 particles in plasma increases rapidly, reaches a peak, and then declines until it reaches a set point level. Understanding the kinetics of primary infection, and its effect on the establishment of chronic infection, is important in defining the early pathogenesis of HIV. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00127-10
更新日期:2010-06-01 00:00:00
abstract::We have analyzed the effects of reducing conditions on the folding of the spike (S) protein and on the intracellular transport of the membrane (M) protein of the mouse hepatitis coronavirus. These proteins differ in their potential to form disulfide bonds in the lumen of the endoplasmic reticulum (ER). Intrachain disu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7394-7401.1993
更新日期:1993-12-01 00:00:00
abstract::Signal peptidase cleavage at the C-prM junction in the flavivirus structural polyprotein is inefficient in the absence of the cytoplasmic viral protease, which catalyzes cleavage at the COOH terminus of the C protein. The signal peptidase cleavage occurs efficiently in circumstances where the C protein is deleted or i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2141-2149.1998
更新日期:1998-03-01 00:00:00
abstract::Alphaherpesviruses are parasites of the peripheral nervous system in their natural hosts. After the initial infection of peripheral tissues such as mucosal cells, these neurotropic viruses will invade the peripheral nervous system that innervates the site of infection via long-distance axonal transport of the viral ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10875-10889.2005
更新日期:2005-09-01 00:00:00
abstract::The ubiquitous human polyomavirus JC (JCV) is a small double-stranded DNA virus that establishes a persistent infection, and it is often transmitted from parents to children. There are at least 14 subtypes of the virus associated with different human populations. Because of its presumed codivergence with humans, JCV h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00441-06
更新日期:2006-10-01 00:00:00
abstract::Vertebrates mount a strong innate immune response against viruses, largely by activating the interferon system. Double-stranded RNA (dsRNA), a common intermediate formed during the life cycle of many viruses, is a potent trigger of this response. In contrast, no general inducible antiviral defense mechanism has been r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10442-10448.2004
更新日期:2004-10-01 00:00:00
abstract::Transmission of arenaviruses from rodent hosts to humans is generally thought to occur through inhalation or ingestion of dust or droplets containing viral particles. Here we demonstrate that two identified arenavirus receptors, alpha-dystroglycan (alpha-DG) and transferrin receptor 1 (TfR1), are expressed in polarize...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00100-08
更新日期:2008-06-01 00:00:00
abstract::The poliovirus polyprotein is cotranslationally linked to myristic acid at its amino-terminal glycine residue. We investigated the role of myristoylation in the viral replication cycle by site-directed mutagenesis of this glycine codon. Synthetic full-length RNA transcripts carrying a Gly-to-Ala mutation (G4002A) gave...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2433-2436.1990
更新日期:1990-05-01 00:00:00
abstract:UNLABELLED:The cellular response to virus infection is initiated when pathogen recognition receptors (PRR) engage viral pathogen-associated molecular patterns (PAMPs). This process results in induction of downstream signaling pathways that activate the transcription factor interferon regulatory factor 3 (IRF3). IRF3 pl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02202-15
更新日期:2015-12-16 00:00:00
abstract::Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes. Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis in humans, does not in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.11.5375-5380.2001
更新日期:2001-06-01 00:00:00
abstract::Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02586-07
更新日期:2008-05-01 00:00:00
abstract::Serial passage at high multiplicity of pseudorabies virus generates defective interfering particles (DIPs) whose genomes consist at least in part of reiterations of segments of DNA in which sequences originating from different regions of the genome have become covalently linked (F. J. Rixon and T. Ben-Porat, Virology ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.318-327.1986
更新日期:1986-08-01 00:00:00
abstract::Rhinoviruses (RV) are the major cause of acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). Rhinoviruses have been shown to activate macrophages, but rhinovirus replication in macrophages has not been reported. Tumor necrosis factor alpha (TNF-alpha) is implicated in the pathogenesis of ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00162-06
更新日期:2006-08-01 00:00:00
abstract::We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05624-11
更新日期:2011-12-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.5.401-408.1968
更新日期:1968-05-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract::Cervical cancer develops through the combined activities of the human papillomavirus (HPV) E6 and E7 oncoproteins. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02074-12
更新日期:2013-02-01 00:00:00
abstract::Among the five serine incorporator (SERINC) family members, SERINC5 (Ser5) was reported to strongly inhibit HIV-1 replication, which is counteracted by Nef. Ser5 produces 5 alternatively spliced isoforms: Ser5-001 has 10 putative transmembrane domains, whereas Ser5-004, -005, -008a, and -008b do not have the last one....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00137-17
更新日期:2017-04-28 00:00:00
abstract::We have previously shown that the nonconserved carboxy-terminal exon of the adenovirus type 2 E1A-289R protein contains two interchangeable sequence elements, auxiliary region (AR) 1 and AR2, that are required for efficient CR3-mediated transcriptional activation of the viral E4 promoter (M. Bondesson, C. Svensson, S....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5978-5983.1998
更新日期:1998-07-01 00:00:00
abstract::The mosquito-transmitted dengue virus (DENV) infects millions of people in tropical and subtropical regions. Maturation of DENV particles requires proper cleavage of the viral polyprotein, including processing of 8 of the 13 substrate cleavage sites by dengue virus NS2B/NS3 protease. With no available direct-acting an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00045-17
更新日期:2017-04-28 00:00:00
abstract::Vaccinia virus J1R protein is required for virion morphogenesis (W. L. Chiu and W. Chang, J. Virol. 76:9575-9587, 2002). In this work, we further characterized the J1R protein of wild-type vaccinia virus and compared it with the protein encoded by the temperature-sensitive mutant virus Cts45. The mutant Cts45 was foun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8046-8056.2005
更新日期:2005-07-01 00:00:00
abstract::The Moloney sarcoma virus-specific onc gene, referred to as v-mos, was used as probe to hybridize to restricted DNAs from various mouse-Chinese hamster hybrid cell lines. These hybrid cells contain, in addition to all of the Chinese hamster chromosomes, various numbers (less than a full complement) of mouse chromosome...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.2.752-754.1982
更新日期:1982-11-01 00:00:00
abstract::In an effort to identify host proteins involved in herpes simplex virus type 1 replication, monkey and human cellular protein activities (called OF-1) that bind the viral replication origin, oriS, have been described. We show by mass spectrometry that the DNA-binding component of human OF-1 contains Ku70 and Ku80 prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7839-7842.2004
更新日期:2004-07-01 00:00:00
abstract::The Tax protein from human T-cell leukemia virus type I (HTLV-I) is a 40-kDa phosphoprotein capable of activating transcription from its own long terminal repeat (LTR), as well as increasing the transcription of cellular genes. Transcriptional activation of the HTLV-I LTR has been demonstrated via a cyclic-AMP-respons...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4436-4441.1993
更新日期:1993-07-01 00:00:00
abstract::2'-5'-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein. Here we describe differential inhibitory activities of human OASL and the two mouse OASL homologs against respiratory syncytial virus (RSV) replication. Interestingly, nonstructural protein 1 (NS1) of RSV promoted proteas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01076-15
更新日期:2015-10-01 00:00:00