Abstract:
:Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. This process is thought to involve RNA secondary and tertiary structural motifs since there is little conservation of the primary sequence of this region between retroviruses. To confirm RNA secondary structures, which we and others have predicted for this region, disruptive, compensatory, and deletion mutations were introduced into proviral constructs, which were then assayed in a permissive cell line. Disruption of either of two predicted stem-loops was found to greatly reduce RNA encapsidation and replication, whereas compensatory mutations restoring base pairing to these stem-loops had a wild-type phenotype. A GGNGR motif was identified in the loops of three hairpins in this region. Results were consistent with the hypothesis that the process of efficient RNA encapsidation is linked to dimerization. Replication and encapsidation were shown to occur at a reduced rate in the absence of the previously described kissing hairpin motif.
journal_name
J Viroljournal_title
Journal of virologyauthors
Harrison GP,Miele G,Hunter E,Lever AMdoi
10.1128/JVI.72.7.5886-5896.1998subject
Has Abstractpub_date
1998-07-01 00:00:00pages
5886-96issue
7eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
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pub_type: 杂志文章
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更新日期:1984-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1746-1752.1988
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1981-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00