Selectable recombinant herpesvirus saimiri is capable of persisting in a human T-cell line.

Abstract:

:Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cells contained episomal viral DNA in high copy number. This selectable vector should be generally applicable for gene expression in human T cells.

journal_name

J Virol

journal_title

Journal of virology

authors

Grassmann R,Fleckenstein B

doi

10.1128/JVI.63.4.1818-1821.1989

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

1818-21

issue

4

eissn

0022-538X

issn

1098-5514

journal_volume

63

pub_type

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