Abstract:
:mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contains the thymidine kinase gene, have been identified and mapped by hybrid-arrested translation and mRNA selection. Such mRNA's, when translated in vitro, directed the synthesis of polypeptides of apparent molecular weights 43,000 (VI43) and 39,000 (VI39). mRNA for enzymatically active thymidine kinase was enriched by more than 20-fold after selection. Mapping was carried out with restriction endonuclease fragments of BamHI p, and locations of the 5' and 3' termini of VI43 mRNA were deduced. Analysis of nucleotide sequences around the 5' terminus revealed several consensus sequences commonly found at the start of eucaryotic mRNA's and which are presumably involved in initiation of transcription by RNA polymerase II. Translation of mRNA's for VI43, VI39, and the thymidine kinase enzyme was arrested only by a 1,170-base-pair region of BamHI p. Since this region is insufficient for adjacent genes, coding sequences for VI43 and VI39 must overlap; the possible relationship of these two polypeptides is discussed. A virus-induced product equivalent to VI39 was detected in infected cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Preston CM,McGeoch DJdoi
10.1128/JVI.38.2.593-605.1981subject
Has Abstractpub_date
1981-05-01 00:00:00pages
593-605issue
2eissn
0022-538Xissn
1098-5514journal_volume
38pub_type
杂志文章abstract:UNLABELLED:Marburg virus (MARV), which belongs to the virus family Filoviridae, causes hemorrhagic fever in humans and nonhuman primates that is often fatal. MARV is a lipid-enveloped virus that during the replication process extracts its lipid coat from the plasma membrane of the host cell it infects. MARV carries sev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02607-15
更新日期:2015-12-30 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00
abstract::Hepatitis C virus (HCV) chronic infection is characterized by low-level or undetectable cellular immune responses against HCV antigens. HCV proteins have been shown to affect various intracellular events and modulate immune responses, although the precise mechanisms used to mediate these effects are not fully understo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02547-07
更新日期:2008-04-01 00:00:00
abstract::Temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups I, II and IV inhibited the replication of wild-type vesicular stomatitis virus when mixed infections were carried out in BHK21 cells at 32, 37, and 39.5 C. The group IV mutant (ts G 41) was most effective in this regar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.1.102-107.1976
更新日期:1976-07-01 00:00:00
abstract::The role of the cell nucleus in the development of African swine fever virus in Vero cells has been studied. No viral growth could be detected in enucleated cells under conditions that allow normal development of Sindbis virus. Furthermore, African swine fever virus DNA synthesis was inhibited more than 95% after infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.902-905.1977
更新日期:1977-03-01 00:00:00
abstract::The activation of the human polyomavirus BK causes polyomavirus-associated nephropathy in immunocompromised humans. Studies of the virus have been restricted since the virus DNA replication is species specific. Cell-based and cell-free DNA replication systems, including the BK virus (BKV) monopolymerase DNA replicatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00527-10
更新日期:2010-07-01 00:00:00
abstract::Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
更新日期:2007-03-01 00:00:00
abstract::The hepatitis C virus (HCV) causes chronic hepatitis in most infected individuals by evading host immune defenses. In this investigation, we show that HCV-infected cells may go undetected in the immune system by suppressing major histocompatibility complex (MHC) class I antigen presentation to cytotoxic T lymphocytes....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11644-11650.2003
更新日期:2003-11-01 00:00:00
abstract::Spleen cells from mice, 4 to 60 days after infection with mouse hepatitis virus type 4, produced interleukin-2, as well as interleukin-3, in the absence of exogenous stimulants in vitro. This unique lymphokine production by mouse hepatitis virus type 4 infection was controlled by host genes. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3506-3508.1988
更新日期:1988-09-01 00:00:00
abstract::Bluetongue virus (BTV), a member of the Reoviridae family, is an insect-borne animal pathogen. Virus release from infected cells is predominantly by cell lysis, but some BTV particles are also released from the plasma membrane. The nonstructural protein NS3 has been implicated in this process. Using alternate initiato...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02352-10
更新日期:2011-05-01 00:00:00
abstract::Host cell interactions of human adenovirus serotypes belonging to subgroups B (adenovirus type 3 [Ad3] and Ad7) and C (Ad2 and Ad5) were comparatively analyzed at three levels: (i) binding of virus particles with host cell receptors; (ii) cointernalization of macromolecules with adenovirions; and (iii) adenovirus-indu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3661-3673.1990
更新日期:1990-08-01 00:00:00
abstract::We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5delta32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral bl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3684-3691.2006
更新日期:2006-04-01 00:00:00
abstract::Rhesus cytomegalovirus (RhCMV) infection of macaques exhibits strong similarities to human CMV (HCMV) persistence and pathogenesis. The immunogenicity of DNA vaccines encoding three RhCMV proteins (a truncated version of glycoprotein B lacking the transmembrane region and endodomain [gBDeltaTM], phosphoprotein 65-2 [p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-06
更新日期:2007-02-01 00:00:00
abstract::The primary herpes simplex virus type 2 (HSV-2) latency-associated transcript (LAT) promoter influences LAT expression and rates of virus reactivation. We explored the biological importance of particular neuronally responsive regions within the promoter by creating new recombinant viruses bearing a targeted deletion (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7903-7910.1997
更新日期:1997-10-01 00:00:00
abstract::Accumulated evidence demonstrates that Japanese encephalitis virus (JEV) infection triggers endoplasmic reticulum (ER) stress and neuron apoptosis. ER stress sensor protein kinase R-like endoplasmic reticulum kinase (PERK) has been reported to induce apoptosis under acute or prolonged ER stress. However, the precise r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00887-19
更新日期:2019-08-13 00:00:00
abstract::HeLa cells infected with human rhinovirus type 2 synthesize a mixture of single-and double-stranded ribonucleic acid (RNA). The RNA synthesized by the membrane-bound RNA polymerase complex in vitro is also a mixture of single- and double-stranded RNA, whereas the deoxycholate-treated RNA polymerase complex synthesized...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.1.93-98.1972
更新日期:1972-07-01 00:00:00
abstract:UNLABELLED:The risk of transmission of transmissible spongiform encephalopathies (TSE) between different species has been notoriously unpredictable because the mechanisms of transmission are not fully understood. A transmission barrier between species often prevents infection of a new host with a TSE agent. Nonetheless...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02296-14
更新日期:2015-05-01 00:00:00
abstract::V2/V3 conformational epitope antibodies that broadly neutralize HIV-1 (PG9 and PG16) have been recently described. Since an elicitation of previously known broadly neutralizing antibodies has proven elusive, the induction of antibodies with such specificity is an important goal for HIV-1 vaccine development. A critica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05045-11
更新日期:2011-10-01 00:00:00
abstract::Diverse members of the Paramyxovirus family of negative-strand RNA viruses effectively suppress host innate immune responses through the actions of their V proteins. The V protein mediates interference with the interferon regulatory RNA helicase MDA5 to avoid cellular antiviral responses. Analysis of the interaction i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00153-09
更新日期:2009-07-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is known to take an endosomal pathway for cell entry; however, it is thought to enter directly from the cell surface when a receptor-bound virion spike (S) protein is affected by trypsin, which induces cleavage of the S protein and activates its fusion po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01412-08
更新日期:2008-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1350-1361.1999
更新日期:1999-02-01 00:00:00
abstract::Serial passage at high multiplicity of pseudorabies virus generates defective interfering particles (DIPs) whose genomes consist at least in part of reiterations of segments of DNA in which sequences originating from different regions of the genome have become covalently linked (F. J. Rixon and T. Ben-Porat, Virology ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.318-327.1986
更新日期:1986-08-01 00:00:00
abstract::Brome mosaic virus (BMV), a member of the alphavirus-like super-family of positive-strand RNA viruses, encodes two proteins required for viral RNA replication: 1a and 2a. 1a contains m7G methyltransferase- and helicase-like domains, while 2a contains a polymerase (pol)-like core flanked by N- and C-terminal extensions...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4729-4736.1996
更新日期:1996-07-01 00:00:00
abstract::Nidoviruses (arteriviruses, coronaviruses, and roniviruses) are a phylogenetically compact but diverse group of positive-strand RNA viruses that includes important human and animal pathogens. Nidovirus RNA synthesis is mediated by a cytoplasmic membrane-associated replication/transcription complex that includes up to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00261-09
更新日期:2009-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) gene expression is highly cell and tissue specific. Cell factor-mediated regulatory interactions are involved in regulating the restricted expression of the HCMV major immediate-early (IE) gene (J. F. Baskar, P. P. Smith, G. Nilaver, R. A. Jupp, S. Hoffmann, N. J. Peffer, D. J. Tenney, A. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.236-244.1998
更新日期:1998-01-01 00:00:00
abstract::Inhibition of hepatitis B virus (HBV) replication and viral clearance from an infected host requires both the innate and adaptive immune responses. Expression of interferon (IFN)-inducible proteasome catalytic and regulatory subunits correlates with the IFN-alpha/beta- and IFN-gamma-mediated noncytopathic inhibition o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01779-06
更新日期:2007-01-01 00:00:00
abstract::In intact Madin-Darby canine kidney (MDCK) cell monolayers, vesicular stomatitis virus (VSV) matures only at basolateral membranes beneath tight junctions, whereas influenza virus buds from apical cell surfaces. Early in the growth cycle, the viral glycoproteins are restricted to the membrane domain from which each vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.848-860.1981
更新日期:1981-12-01 00:00:00
abstract::Approximately 10% of gastric carcinomas (GC) are comprised of cells latently infected with Epstein-Barr virus (EBV); however, the mechanism by which EBV contributes to the development of this malignancy is unclear. We have investigated the cellular effects of the only EBV nuclear protein expressed in GC, EBNA1, focusi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05623-11
更新日期:2012-01-01 00:00:00
abstract::Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1). Elevated expression of vascular endothelial growth factor (VEGF) in ATL patients is associated with leukemic cell invasion and infiltration in different organs. The regulatory protein Tax 1 encoded by HTLV-1 plays a pivotal role in T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02166-09
更新日期:2010-05-01 00:00:00
abstract:UNLABELLED:As a herpesvirus, Epstein-Barr virus (EBV) establishes a latent infection that can periodically undergo reactivation, resulting in lytic replication and the production of new infectious virus. Latent membrane protein-1 (LMP1), the principal viral oncoprotein, is a latency-associated protein implicated in reg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00711-15
更新日期:2015-08-01 00:00:00