Characterization of herpes simplex virus 2 temperature-sensitive mutants whose lesions map in or near the coding sequences for the major DNA-binding protein.

abstract：:Chimeric bovine respiratory syncytial viruses (BRSV) expressing glycoproteins of bovine parainfluenza virus type 3 (BPIV-3) instead of BRSV glycoproteins were generated from cDNA. In the BRSV antigenome cDNA, the open reading frames of the major BRSV glycoproteins, attachment protein G and fusion protein F, were repla...

journal_title：Journal of virology

authors： Stope MB,Karger A,Schmidt U,Buchholz UJ

更新日期：2001-10-01 00:00:00

abstract：:Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory nerve ganglia, but the characteristics of VZV latency are not well defined. Immunohistochemical detection of the VZV immediate-early 63 (IE63) protein in ganglion neurons has been described, but there are significant discrepancies in esti...

journal_title：Journal of virology

authors： Zerboni L,Sobel RA,Ramachandran V,Rajamani J,Ruyechan W,Abendroth A,Arvin A

更新日期：2010-04-01 00:00:00

abstract：:The human adenovirus type 2 (Ad2) mutant Ad2ts111 has previously been shown to contain two mutations which result in a complex phenotype. Ad2ts111 contains a single base change in the early region 1B (E1B) 19,000-molecular-weight (19K) coding region which yields a cyt deg phenotype and another defect which maps to the...

journal_title：Journal of virology

authors： Prelich G,Stillman BW

更新日期：1986-03-01 00:00:00

abstract：:Blattella germanica densovirus (BgDNV) is an autonomous parvovirus that infects the German cockroach. BgDNV possesses three mRNAs for NS proteins, two of which are splice variants of the unspliced transcript. The unspliced variant encodes open reading frame 5 (ORF5) (NS3), while NSspl1 encodes ORF3 (NS1) and ORF4 (NS2...

journal_title：Journal of virology

authors： Kapelinskaya TV,Martynova EU,Schal C,Mukha DV

更新日期：2011-11-01 00:00:00

abstract：:The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...

journal_title：Journal of virology

authors： Lee TH,Elledge SJ,Butel JS

更新日期：1995-02-01 00:00:00

abstract：:The human foamy virus (HFV) contains within the U3 region of its long terminal repeat (LTR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as...

journal_title：Journal of virology

authors： Maurer B,Serfling E,ter Meulen V,Rethwilm A

更新日期：1991-11-01 00:00:00

abstract：:Herpes simplex virus (HSV) has often been suggested for development as a vector, particularly for the nervous system. Considerable evidence has shown that for use of HSV as a vector, immediate-early (IE) gene expression must be minimized or abolished, otherwise such vectors are likely to be highly cytotoxic. Mutations...

journal_title：Journal of virology

authors： Thomas SK,Lilley CE,Latchman DS,Coffin RS

更新日期：1999-09-01 00:00:00

abstract：:We have used immunofluorescence in parallel with transmission and scanning electron microscopy to characterize the unusual cytoplasmic and nucleolar accumulation of Simian virus 40 (SV40) virion protein (C antigen) at restrictive temperatures (39 to 41 C) in monkey cells infected with a temperature-sensitive mutant of...

journal_title：Journal of virology

authors： Widmer C,Robb JA

更新日期：1974-12-01 00:00:00

abstract：:The great majority of viral mRNAs in mouse C127 cells transformed by bovine papillomavirus type 1 (BPV) have a common 3' end at the early polyadenylation site which is 23 nucleotides (nt) downstream of a canonical poly(A) consensus signal. Twenty percent of BPV mRNA from productively infected cells bypasses the early ...

journal_title：Journal of virology

authors： Andrews EM,DiMaio D

更新日期：1993-12-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome (PRRS) consistently elevates the frequency of disease and mortality in young pigs. Many different secondary bacterial diseases occur in PRRS virus (PRRSV)-infected pigs. However, to date, establishing a reproducible experimental model of PRRSV infection in weaned pigs, wit...

journal_title：Journal of virology

authors： Feng W,Laster SM,Tompkins M,Brown T,Xu JS,Altier C,Gomez W,Benfield D,McCaw MB

更新日期：2001-05-01 00:00:00

abstract：:Cellular and viral factors participate in the replication cycle of rotavirus. We report that the guanine nucleotide exchange factor GBF1, which activates the small GTPase Arf1 to induce COPI transport processes, is required for rotavirus replication since knocking down GBF1 expression by RNA interference or inhibiting...

journal_title：Journal of virology

authors： Martínez JL,Arnoldi F,Schraner EM,Eichwald C,Silva-Ayala D,Lee E,Sztul E,Burrone ÓR,López S,Arias CF

更新日期：2019-09-12 00:00:00

abstract：:The regulation of murine cytomegalovirus early (E) gene expression was studied in the cell line B25, which is stably transfected with the immediate-early ie1/ie3 gene complex. Infection of B25 cells in the presence of the protein synthesis inhibitor cycloheximide resulted in the expression of some E genes, whereas for...

journal_title：Journal of virology

authors： Bühler B,Keil GM,Weiland F,Koszinowski UH

更新日期：1990-05-01 00:00:00

abstract：:Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active ...

journal_title：Journal of virology

authors： Zhong W,Gutshall LL,Del Vecchio AM

更新日期：1998-11-01 00:00:00

abstract：:The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein...

journal_title：Journal of virology

authors： Privalsky ML,Penhoet EE

更新日期：1977-10-01 00:00:00

abstract：:We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...

journal_title：Journal of virology

authors： Lee PS,Zhu X,Yu W,Wilson IA

更新日期：2015-07-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is an oncogenic human herpesvirus that dramatically reorganizes host gene expression to immortalize primary B cells. In this study, we analyzed EBV-regulated host gene expression changes following primary B-cell infection, both during initial proliferation and through transformation into lymph...

journal_title：Journal of virology

authors： Price AM,Tourigny JP,Forte E,Salinas RE,Dave SS,Luftig MA

更新日期：2012-10-01 00:00:00

abstract：:The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping c...

journal_title：Journal of virology

authors： van Zijl M,Quint W,Briaire J,de Rover T,Gielkens A,Berns A

更新日期：1988-06-01 00:00:00

abstract：:Influenza A viruses (IAVs) cause seasonal epidemics and occasional pandemics, representing a serious public health concern. It has been described that one mechanism used by some IAV strains to escape the host innate immune responses and modulate virus pathogenicity involves the ability of the PA-X and NS1 proteins to ...

journal_title：Journal of virology

authors： Nogales A,Rodriguez L,DeDiego ML,Topham DJ,Martínez-Sobrido L

更新日期：2017-08-10 00:00:00

abstract：:The development of antiviral drug resistance is an important problem in the treatment of human immunodeficiency virus type 1 (HIV-1) infection. Potent antiretroviral therapy is currently used for treatment, and typically consists of at least two reverse transcriptase (RT) inhibitors. We have previously reported that b...

journal_title：Journal of virology

authors： Chen R,Yokoyama M,Sato H,Reilly C,Mansky LM

更新日期：2005-09-01 00:00:00

abstract：:Murine gammaherpesvirus 68 (MHV68) is a gammaherpesvirus that was first isolated from murid rodents. MHV68 establishes a latent infection in the spleen and other lymphoid organs. Several gammaherpesviruses, including herpesvirus saimiri, human herpesvirus 8, and MHV68, encode proteins with extensive homology to the D-...

journal_title：Journal of virology

authors： Hoge AT,Hendrickson SB,Burns WH

更新日期：2000-08-01 00:00:00

abstract：:The effect of bacteriophage SPO1 infection of Bacillus subtilis and a deoxyribonucleic acid (DNA) polymerase-deficient (pol(-)) mutant of this microorganism on the synthesis of DNA has been examined. Soon after infection, the incorporation of deoxyribonucleoside triphosphates into acid-insoluble material by cell lysat...

journal_title：Journal of virology

authors： Yehle CO,Ganesan AT

更新日期：1972-02-01 00:00:00

abstract：:Nipah virus (NiV), of the family Paramyxoviridae, was isolated in 1999 in Malaysia from a human fatality in an outbreak of severe human encephalitis, when human infections were linked to transmission of the virus from pigs. Consequently, a swine vaccine able to abolish virus shedding is of veterinary and human health ...

journal_title：Journal of virology

authors： Weingartl HM,Berhane Y,Caswell JL,Loosmore S,Audonnet JC,Roth JA,Czub M

更新日期：2006-08-01 00:00:00

abstract：:Lipid rafts are proposed to function as platforms for both receptor signaling and trafficking. Following interaction with antigenic peptides, the T-cell receptor (TCR) rapidly translocates to lipid rafts, where it transmits signals and subsequently undergoes endocytosis. The Tip protein of herpesvirus saimiri (HVS), w...

journal_title：Journal of virology

authors： Park J,Cho NH,Choi JK,Feng P,Choe J,Jung JU

更新日期：2003-08-01 00:00:00

abstract：:Wild-type clones of reovirus serotypes 1 (Lang), 2 (Jones), and 3 (Dearing) were serially passaged in L cells at a high multiplicity of infection, and the virus population was examined at passage levels 2, 5, and 11 for the presence of temperature-sensitive (ts) mutants. By passage 11 all three serotypes contained ts ...

journal_title：Journal of virology

authors： Ahmed R,Chakraborty PR,Fields BN

更新日期：1980-04-01 00:00:00

abstract：:Human immunodeficiency virus type 1 contains a transmembrane glycoprotein with an unusually long cytoplasmic domain. To determine the role of this domain in virus replication, a series of single nucleotide changes that result in the insertion of premature termination codons throughout the cytoplasmic domain has been c...

journal_title：Journal of virology

authors： Dubay JW,Roberts SJ,Hahn BH,Hunter E

更新日期：1992-11-01 00:00:00

abstract：:Hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). In this report, we demonstrate that the cellular entry of HFRS-associated hantaviruses is facilitated by specific integrins expressed on plate...

journal_title：Journal of virology

authors： Gavrilovskaya IN,Brown EJ,Ginsberg MH,Mackow ER

更新日期：1999-05-01 00:00:00

abstract：UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...

journal_title：Journal of virology

authors： Yang E,Arvin AM,Oliver SL

更新日期：2016-07-27 00:00:00

abstract：:Intercellular adhesion molecule 1 (ICAM-1) mediates binding and entry of major group human rhinoviruses (HRVs). Whereas the entry pathway of minor group HRVs has been studied in detail and is comparatively well understood, the pathway taken by major group HRVs is largely unknown. Use of immunofluorescence microscopy, ...

journal_title：Journal of virology

authors： Khan AG,Pickl-Herk A,Gajdzik L,Marlovits TC,Fuchs R,Blaas D

更新日期：2010-04-01 00:00:00

abstract：:Rfv3 is an autosomal dominant gene that influences the recovery of resistant mice from Friend retrovirus (FV) infection by limiting viremia and promoting a more potent neutralizing antibody response. We previously reported that Rfv3 is encoded by Apobec3, an innate retrovirus restriction factor. However, it was recent...

journal_title：Journal of virology

authors： Santiago ML,Smith DS,Barrett BS,Montano M,Benitez RL,Pelanda R,Hasenkrug KJ,Greene WC

更新日期：2011-01-01 00:00:00

abstract：:Unlike RNA viruses, most DNA viruses replicate their genomes with high-fidelity polymerases that rarely make base substitution errors. Nevertheless, experimental evolution studies have revealed rapid acquisition of adaptive mutations during serial passage of attenuated vaccinia virus (VACV). One way in which adaptatio...

journal_title：Journal of virology

authors： Senkevich TG,Zhivkoplias EK,Weisberg AS,Moss B

更新日期：2020-08-31 00:00:00