Abstract:
:The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog of the UV-damaged DNA-binding protein (UV-DDB) recovered from a monkey cell cDNA library. UV-DDB is presumed to be involved in DNA repair. The interaction between X protein and XAP-1 protein was verified by immunoprecipitation of yeast cell lysates expressing both proteins and by in vitro mixing with X protein expressed as a glutathione S-transferase fusion protein and XAP-1 protein either in HeLa cell extracts or synthesized by in vitro translation. We speculate that the interaction of X protein with a DNA repair protein may recruit cellular proteins to repair the partially double-stranded HBV genome or may modify cellular transcription processes. An effect on the cellular DNA repair system may explain a cofactor role for HBV in liver cancer development.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lee TH,Elledge SJ,Butel JSdoi
10.1128/JVI.69.2.1107-1114.1995subject
Has Abstractpub_date
1995-02-01 00:00:00pages
1107-14issue
2eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.75-84.1975
更新日期:1975-07-01 00:00:00
abstract::Previous studies using wild-type Encephalomyocarditis virus (EMCV) and Mengo virus, which have long poly(C) tracts (61 to 146 C's) at the 5' nontranslated region of the genome, and variants of these viruses genetically engineered to truncate or substitute the poly(C) tracts have produced conflicting data on the role o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9136-9146.2003
更新日期:2003-09-01 00:00:00
abstract::Most poxviruses express multiple proteins containing ankyrin (ANK) repeats accounting for a large superfamily of related but unique determinants of poxviral tropism. Recently, select members of this novel family of poxvirus proteins have drawn considerable attention for their potential roles in modulating intracellula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00963-09
更新日期:2009-12-01 00:00:00
abstract::In herpes simplex virus type 2, the mRNAs of ICP4 and ICP22/47 are divergently transcribed and their transcription initiation sites are separated by 750 base pairs (L. J. Whitton and J. B. Clements, Nucleic Acids Res. 12:2061-2078, 1984). This 750-base-pair region contains many recognized cis-acting elements, includin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2036-2047.1989
更新日期:1989-05-01 00:00:00
abstract::Western equine encephalitis virus (WEEV; Togaviridae, Alphavirus) is an enveloped RNA virus that is typically transmitted to vertebrate hosts by infected mosquitoes. WEEV is an important cause of viral encephalitis in humans and horses in the Americas, and infection results in a range of disease, from mild flu-like il...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00876-10
更新日期:2010-10-01 00:00:00
abstract::The positive-strand RNA bromoviruses encode two nonstructural proteins, 1a and 2a, involved in RNA-dependent RNA replication. These proteins have extensive sequence similarities with methyltransferase, helicase, and polymerase proteins of other plant and animal viruses. 1a and 2a can also form a complex in vitro. To e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7181-7189.1993
更新日期:1993-12-01 00:00:00
abstract::Measles virus has two glycoproteins. The larger glycoprotein (HA) is composed of 76,000-dalton subunits that are bound by disulfide bonds. The smaller glycoprotein (F) appears to contain a glucosamine-rich portion that is linked to an unglycosylated protein by disulfide bonds. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.2.687-692.1978
更新日期:1978-02-01 00:00:00
abstract::The glycan shield of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein serves as a barrier to antibody-mediated neutralization and plays a critical role in transmission and infection. One of the few broadly neutralizing HIV-1 antibodies, 2G12, binds to a carbohydrate epitope consisting of an array...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00412-08
更新日期:2008-07-01 00:00:00
abstract::The mechanism of regulation for the ribozyme activity of hepatitis delta virus (HDV) RNA in infected cells is unknown. Previously, we developed a direct assay capable of detecting the ribozyme activity of HDV dimer or trimer RNAs in vivo (K.-S. Jeng, A. Daniel, and M. M. C. Lai, J. Virol, 70:2403-2410, 1996). In this ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4205-4209.1996
更新日期:1996-07-01 00:00:00
abstract::RNA viruses within a host exist as dynamic distributions of closely related mutants and recombinant genomes. These closely related mutants and recombinant genomes, which are subjected to a continuous process of genetic variation, competition, and selection, act as a unit of selection, termed viral quasispecies. Charac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00950-09
更新日期:2009-12-01 00:00:00
abstract::The dengue type 4 virus (DEN4) genome contains a 384-nucleotide (nt) 3' noncoding sequence in which the last 81 nt, predicted to form a secondary structure, are thought to be essential for virus replication. Immediately upstream of the secondary structure, short RNA sequences that are conserved among mosquito-borne fl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
更新日期:1996-06-01 00:00:00
abstract::Interleukin-15 (IL-15), natural killer (NK) cells, and NK T (NKT) cells, components of the innate immune system, are known to contribute to defense against pathogens, including viruses. Here we report that IL-15(-/-) (NK(-) and NKT(-/+)) mice and RAG-2(-/-)/gamma(c)(-/-) (NK(-) and NKT(-)) mice that lack all lymphoid ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::Several reports have shown that human cytomegalovirus (HCMV)-infected cells are resistant to NK lysis. These studies have focused on receptor-ligand interactions, and different HCMV proteins have been indicated to mediate inhibitory NK signals. Here, we report that the HCMV protein UL16 is of major importance for the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.4539-4545.2003
更新日期:2003-04-01 00:00:00
abstract:UNLABELLED:Gammaherpesviruses establish persistent, systemic infections and cause cancers. Murid herpesvirus 4 (MuHV-4) provides a unique window into the early events of host colonization. It spreads via lymph nodes. While dendritic cells (DC) pass MuHV-4 to lymph node B cells, subcapsular sinus macrophages (SSM), whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01108-16
更新日期:2016-09-29 00:00:00
abstract::Nonstructural protein 1 (NS1) of yellow fever virus (YF) is a glycoprotein localized to extracytoplasmic compartments within infected cells. We have previously shown that NS1 can be supplied in trans and is required for viral RNA replication, a process thought to occur in membrane-bound cytoplasmic complexes. Here we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4611-4621.1999
更新日期:1999-06-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus termed SARS-CoV. We and others have previously shown that the replication of SARS-CoV can be suppressed by exogenously added interferon (IFN), a cytokine which is normally synthesized by cells as a reaction to virus infection. Here, we demonstra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2079-2086.2005
更新日期:2005-02-01 00:00:00
abstract::The RNA elements that are required for replication of defective interfering (DI) RNA of the JHM strain of mouse hepatitis virus (MHV) consist of three discontinuous genomic regions: about 0.46 to 0.47 kb from both terminal sequences and an internal 58-nucleotide (nt)-long sequence (58-nt region) present at about 0.9 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.7926-7933.1998
更新日期:1998-10-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infection is well controlled mainly by cytotoxic CD8(+) T lymphocytes (CTL) directed against the matrix protein pp65 despite the numerous immune escape mechanisms developed by the virus. Dendritic cells (DCs) are key antigen-presenting cells for the generation of an immune response which h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.10018-10024.2000
更新日期:2000-11-01 00:00:00
abstract::The relative contributions of interleukin-12 (IL-12) and IL-23 to viral pathogenesis have not been extensively studied. IL-12p40 mRNA rapidly increases after neurotropic coronavirus infection. Infection of mice defective in both IL-12 and IL-23 (p40(-/-)), in IL-12 alone (p35(-/-)), and in IL-23 alone (p19(-/-)) revea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00315-09
更新日期:2009-06-01 00:00:00
abstract::The contributions of the glycoproteins gI (ORF67) and gE (ORF68) to varicella-zoster virus (VZV) replication were investigated in deletion mutants made by using cosmids with VZV DNA derived from the Oka strain. Deletion of both gI and gE prevented virus replication. Complete deletion of gI or deletions of 60% of the N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8279-8288.1997
更新日期:1997-11-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-drive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4565-4567.1990
更新日期:1990-09-01 00:00:00
abstract::Human immunodeficiency virus clade C (HIV-C) accounts for >56% of all HIV infections worldwide. To investigate vaccine safety and efficacy in nonhuman primates, a pathogenic, R5-tropic, neutralization-sensitive simian-human immunodeficiency virus (SHIV) carrying HIV-C env would be desirable. We have constructed SHIV-2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02066-08
更新日期:2009-02-01 00:00:00
abstract::Interallelic complementation between certain temperature-sensitive mutants of gene 42 of bacteriophage T4 was demonstrated by measuring the incorporation of labeled thymine into DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.415-416.1973
更新日期:1973-08-01 00:00:00
abstract::We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.4021-4024.1990
更新日期:1990-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00
abstract:UNLABELLED:Herpes simplex virus 1 (HSV-1) most commonly causes recrudescent labial ulcers; however, it is also the leading cause of infectious blindness in developed countries. Previous research in animal models has demonstrated that the severity of HSV-1 ocular disease is influenced by three main factors: host innate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00821-16
更新日期:2016-08-26 00:00:00
abstract::Recombinant retroviruses containing the trans activator genes of human T-cell leukemia virus (HTLV) type II and human T-cell lymphotropic virus type III were constructed. The trans activator genes tat II and tat III were inserted into the murine retroviral vector pZIPNEOSV(X)1. Recombinant plasmids were transfected in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.379-384.1986
更新日期:1986-01-01 00:00:00
abstract::We report a Sendai virus (SeV) vector system for expression of major histocompatibility complex (MHC) class I/peptide complexes. We cloned the extracellular domain of a human MHC class I heavy chain, HLA-A*2402, and human beta-2 microglobulin (beta2m) fused with HLA-A*2402-restricted human immunodeficiency virus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11982-11988.2002
更新日期:2002-12-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
abstract::Cholesterol plays an essential role in the life cycle of several enveloped viruses. Many of these viruses manipulate host cholesterol metabolism to facilitate their replication. HIV-1 infection of CD4(+) T cells activates the sterol regulatory element-binding protein 2 (SREBP2) transcriptional program, which includes ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00337-11
更新日期:2011-08-01 00:00:00