Abstract:
:Several reports have shown that human cytomegalovirus (HCMV)-infected cells are resistant to NK lysis. These studies have focused on receptor-ligand interactions, and different HCMV proteins have been indicated to mediate inhibitory NK signals. Here, we report that the HCMV protein UL16 is of major importance for the ability of HCMV-infected cells to resist NK cell-mediated cytotoxicity. Fibroblasts infected with the UL16 deletion mutant HCMV strain exhibited a 70% increased sensitivity to NK killing at 7 days postinfection compared to AD169-infected cells. Interestingly, HCMV-infected cells did not appear to engage inhibitory molecules on NK cells, since the levels of granzyme B were not reduced in supernatants obtained from NK cell cocultures with infected target cells compared to uninfected target cells. Furthermore, HCMV-infected cells, but not cells infected with the UL16 deletion mutant HCMV strain, exhibited a significantly increased resistance to the action of cytolytic proteins, including perforin, granzyme B, streptolysin O, and porcine NK lysin. In addition, fluorescence-activated cell sorting for UL16-positive transfected cells resulted in protection levels of 90% compared to control cells carrying the green fluorescent protein vector. Thus, the UL16 protein mediates an increased protection against the action of cytolytic proteins released by activated NK cells, possibly by a membrane-stabilizing mechanisms, rather than by delivering negative signals to NK cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Odeberg J,Browne H,Metkar S,Froelich CJ,Brandén L,Cosman D,Söderberg-Nauclér Cdoi
10.1128/jvi.77.8.4539-4545.2003subject
Has Abstractpub_date
2003-04-01 00:00:00pages
4539-45issue
8eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.954-963.1975
更新日期:1975-04-01 00:00:00
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更新日期:2016-03-28 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00