Abstract:
:Rabies, as the oldest known infectious disease, remains a serious threat to public health worldwide. The eukaryotic cytosolic chaperonin TRiC/CCT complex facilitates the folding of proteins through ATP hydrolysis. Here, we investigated the expression, cellular localization, and function of neuronal CCTγ during neurotropic rabies virus (RABV) infection using mouse N2a cells as a model. Following RABV infection, 24 altered proteins were identified by using two-dimensional electrophoresis and mass spectrometry, including 20 upregulated proteins and 4 downregulated proteins. In mouse N2a cells infected with RABV or cotransfected with RABV genes encoding nucleoprotein (N) and phosphoprotein (P), confocal microscopy demonstrated that upregulated cellular CCTγ was colocalized with viral proteins N and P, which formed a hollow cricoid inclusion within the region around the nucleus. These inclusions, which correspond to Negri bodies (NBs), did not form in mouse N2a cells only expressing the viral protein N or P. Knockdown of CCTγ by lentivirus-mediated RNA interference led to significant inhibition of RABV replication. These results demonstrate that the complex consisting of viral proteins N and P recruits CCTγ to NBs and identify the chaperonin CCTγ as a host factor that facilitates intracellular RABV replication. This work illustrates how viruses can utilize cellular chaperonins and compartmentalization for their own benefit.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zhang J,Wu X,Zan J,Wu Y,Ye C,Ruan X,Zhou Jdoi
10.1128/JVI.03186-12subject
Has Abstractpub_date
2013-07-01 00:00:00pages
7608-21issue
13eissn
0022-538Xissn
1098-5514pii
JVI.03186-12journal_volume
87pub_type
杂志文章abstract::The time course and extent of mengovirus production were the same in metaphase-arrested and interphase L-cells, yet the expression of cytopathology was delayed several hours in metaphase cells. ...
journal_title:Journal of virology
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doi:10.1128/JVI.5.2.262-263.1970
更新日期:1970-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7839-7842.2004
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.5171-5176.1995
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7965-7973.1996
更新日期:1996-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7080-7088.1992
更新日期:1992-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9474-9486.2004
更新日期:2004-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.1-11.1982
更新日期:1982-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.847-853.1982
更新日期:1982-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.75.1.303-310.2001
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.326-329.1982
更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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