Galectin-9 functionally impairs natural killer cells in humans and mice.

Abstract:

:Galectin-9 is a pleiotropic immune modulator affecting numerous cell types of innate and adaptive immunity. Patients with chronic infection with either hepatitis C virus (HCV) or HIV have elevated circulating levels. Limited data exist on the regulation of natural killer (NK) cell function through interaction with galectin-9. We found that galectin-9 ligation downregulates multiple immune-activating genes, including eight involved in the NK cell-mediated cytotoxicity pathway, impairs lymphokine-activated killing, and decreases the proportion of gamma interferon (IFN-γ)-producing NK cells that had been stimulated with interleukin-12 (IL-12)/IL-15. We demonstrate that the transcriptional and functional changes induced by galectin-9 are independent of Tim-3. Consistent with these results for humans, we find that the genetic absence of galectin-9 in mice is associated with greater IFN-γ production by NK cells and enhanced degranulation. We also show that in the setting of a short-term (4-day) murine cytomegalovirus infection, terminally differentiated NKs accumulate in the livers of galectin-9 knockout mice, and that hepatic NKs spontaneously produce significantly more IFN-γ in this setting. Taken together, our results indicate that galectin-9 engagement impairs the function of NK cells, including cytotoxicity and cytokine production.

journal_name

J Virol

journal_title

Journal of virology

authors

Golden-Mason L,McMahan RH,Strong M,Reisdorph R,Mahaffey S,Palmer BE,Cheng L,Kulesza C,Hirashima M,Niki T,Rosen HR

doi

10.1128/JVI.01085-12

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

4835-45

issue

9

eissn

0022-538X

issn

1098-5514

pii

JVI.01085-12

journal_volume

87

pub_type

杂志文章
  • Levels of Epstein-Barr virus DNA in lymphoblastoid cell lines are correlated with frequencies of spontaneous lytic growth but not with levels of expression of EBNA-1, EBNA-2, or latent membrane protein.

    abstract::The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.1.437-444.1990

    authors: Metzenberg S

    更新日期:1990-01-01 00:00:00

  • Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells.

    abstract::Herpesvirus saimiri (HVS) is a T-cell-specific transforming and oncogenic virus. A protein encoded by HVS known as Tip-484 (for tyrosine kinase interacting protein from HVS strain 484) is required for this transformation. Tip-484 binds specifically to the nonreceptor protein tyrosine kinase p56lck. By transfecting Tip...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.1.378-382.1997

    authors: Lund T,Medveczky MM,Medveczky PG

    更新日期:1997-01-01 00:00:00

  • Reversion of Q beta RNA phage mutants by homologous RNA recombination.

    abstract::Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.4.2435-2442.1992

    authors: Palasingam K,Shaklee PN

    更新日期:1992-04-01 00:00:00

  • Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies.

    abstract::Monoclonal antibodies to varicella-zoster virus were used to study viral glycoproteins by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Based on the viral glycoproteins immunoprecipitated, the five monoclonal antibodies fell into three groups. Two antibodies, 4B7 and 8G9 (group 1),...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.52.1.55-62.1984

    authors: Forghani B,Dupuis KW,Schmidt NJ

    更新日期:1984-10-01 00:00:00

  • Distribution of Hepatitis B Virus Nuclear DNA.

    abstract::Chronic hepatitis B affects over 300 million people who are at risk of developing liver cancer. The basis for the persistence of hepatitis B virus (HBV) in hepatocytes, even in the presence of available antiviral therapies, lies in the accumulation of covalently closed circular DNA (cccDNA) in nuclei of infected cells...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01391-17

    authors: Li M,Sohn JA,Seeger C

    更新日期:2017-12-14 00:00:00

  • Generation of an inverting herpes simplex virus 1 mutant lacking the L-S junction a sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the alpha 47 gene.

    abstract::The herpes simplex virus genome consists of two components, L and S, that invert relative to each other to yield four isomeric arrangements, prototype (P), inversion of the S component (Is), inversion of the L component (Il), and inversion of both components (Isl). Previous studies have shown that the 500-base-pair a ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.58.2.583-591.1986

    authors: Longnecker R,Roizman B

    更新日期:1986-05-01 00:00:00

  • Decline in CD4+ cell numbers in cats with naturally acquired feline immunodeficiency virus infection.

    abstract::T-cell subsets were studied by fluorescence-activated cell sorter analysis in 57 feline immunodeficiency virus (FIV)-seropositive cats with naturally acquired FIV infection to see whether CD4(+)-CD8+ alterations were comparable to those observed in human immunodeficiency virus-infected patients. CD4+ values were decre...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.3.1484-1488.1992

    authors: Hoffmann-Fezer G,Thum J,Ackley C,Herbold M,Mysliwietz J,Thefeld S,Hartmann K,Kraft W

    更新日期:1992-03-01 00:00:00

  • Downregulation of protein disulfide isomerase inhibits infection by the mouse polyomavirus.

    abstract::Early stages of infection by the mouse polyomavirus have been studied using HeLa cells stably expressing small interfering RNA to protein disulfide isomerase (PDI). Infectibility measured by nuclear T antigen expression was reduced commensurately with the degree of PDI downregulation. Infectibility was restored by tra...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01117-06

    authors: Gilbert J,Ou W,Silver J,Benjamin T

    更新日期:2006-11-01 00:00:00

  • Murine sarcoma virus ts110 RNA transcripts: origin from a single proviral DNA and sequence of the gag-mos junctions in both the precursor and spliced viral RNAs.

    abstract::Our previous studies have argued persuasively that in murine sarcoma virus ts110 (MuSVts110) the gag and mos genes are fused out of frame due to a approximately 1.5-kilobase (kb) deletion of wild-type murine sarcoma virus 349 (MuSV-349) viral information. As a consequence of this deletion, infected cells grown at 39 d...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.53.2.624-633.1985

    authors: Nash MA,Brizzard BL,Wong JL,Murphy EC Jr

    更新日期:1985-02-01 00:00:00

  • The ABCs of rhinoviruses, wheezing, and asthma.

    abstract::Human rhinoviruses (HRVs) were discovered as common cold pathogens over 50 years ago. Recent advances in molecular viral diagnostics have led to an appreciation of their role in more-significant respiratory illnesses, including bronchiolitis in infancy, childhood pneumonia, and acute exacerbations of chronic respirato...

    journal_title:Journal of virology

    pub_type: 杂志文章,评审

    doi:10.1128/JVI.02290-09

    authors: Gern JE

    更新日期:2010-08-01 00:00:00

  • Live covisualization of competing adeno-associated virus and herpes simplex virus type 1 DNA replication: molecular mechanisms of interaction.

    abstract::We performed live cell visualization assays to directly assess the interaction between competing adeno-associated virus (AAV) and herpes simplex virus type 1 (HSV-1) DNA replication. Our studies reveal the formation of separate AAV and HSV-1 replication compartments and the inhibition of HSV-1 replication compartment ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02476-06

    authors: Glauser DL,Strasser R,Laimbacher AS,Saydam O,Clément N,Linden RM,Ackermann M,Fraefel C

    更新日期:2007-05-01 00:00:00

  • Herpesvirus saimiri oncogene STP-C488 encodes a phosphoprotein.

    abstract::The STP-C488 open reading frame of herpesvirus saimiri encodes an oncoprotein that has transforming and tumor-inducing activities independent of the rest of the herpesvirus genome. STP-C488 protein has an unusual, membrane-associated, fibrous structure and is located primarily in perinuclear compartments. We now repor...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.3.1777-1780.1992

    authors: Jung JU,Desrosiers RC

    更新日期:1992-03-01 00:00:00

  • Persistent West Nile virus associated with a neurological sequela in hamsters identified by motor unit number estimation.

    abstract::To investigate the hypothesis that neurological sequelae are associated with persistent West Nile virus (WNV) and neuropathology, we developed an electrophysiological motor unit number estimation (MUNE) assay to measure the health of motor neurons temporally in hamsters. The MUNE assay was successful in identifying ch...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00017-09

    authors: Siddharthan V,Wang H,Motter NE,Hall JO,Skinner RD,Skirpstunas RT,Morrey JD

    更新日期:2009-05-01 00:00:00

  • Suppression of cellular proliferation by the papillomavirus E2 protein.

    abstract::Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.12.7791-7799.1995

    authors: Dowhanick JJ,McBride AA,Howley PM

    更新日期:1995-12-01 00:00:00

  • Inhibition of HIV-1 Tat-mediated transcription by a coumarin derivative, BPRHIV001, through the Akt pathway.

    abstract::The human immunodeficiency virus type 1 (HIV-1)-encoded RNA-binding protein Tat is known to play an essential role in viral gene expression. In the search for novel compounds to inhibit Tat transactivity, one coumarin derivative, BPRHIV001, was identified, with a 50% effective concentration (EC(50)) against HIV-1 at 1...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00175-11

    authors: Lin PH,Ke YY,Su CT,Shiao HY,Hsieh HP,Chao YK,Lee CN,Kao CL,Chao YS,Chang SY

    更新日期:2011-09-01 00:00:00

  • Transfer of a mutant dihydrofolate reductase gene into pre- and postimplantation mouse embryos by a replication-competent retrovirus vector.

    abstract::In order to explore the potential of retrovirus vectors for efficiently transferring foreign genes into mouse embryos, a replication-competent recombinant Moloney murine leukemia virus (Mo-MLV) vector carrying a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat was us...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.11.4857-4865.1989

    authors: Stuhlmann H,Jaenisch R,Mulligan RC

    更新日期:1989-11-01 00:00:00

  • Cytomegalovirus inhibits the engraftment of donor bone marrow cells by downregulation of hemopoietin gene expression in recipient stroma.

    abstract::Cytomegalovirus (CMV) disease after bone marrow (BM) transplantation is often associated with BM graft failure. There are two possible reasons for such a correlation. First, a poor hematopoietic reconstitution of unrelated etiology could promote the progression of CMV infection by the lack of immune control. Alternati...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.6.5006-5015.1998

    authors: Steffens HP,Podlech J,Kurz S,Angele P,Dreis D,Reddehase MJ

    更新日期:1998-06-01 00:00:00

  • Dexamethasone stimulates expression of transposable type A intracisternal retroviruslike genes in mouse (Mus musculus) cells.

    abstract::Dexamethasone treatment enhances expression of transposable intracisternal type A particles (IAP) at RNA and proteins levels in a murine retrovirus-transformed cell line (Ki-BALB). This effect was ascertained by electron microscopic numeration of IAP. By sequence comparison, we located glucocorticoid-responsive elemen...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.10.3867-3869.1988

    authors: Emanoil-Ravier R,Mercier G,Canivet M,Garcette M,Lasneret J,Peronnet F,Best-Belpomme M,Peries J

    更新日期:1988-10-01 00:00:00

  • Structural variations in species B adenovirus fibers impact CD46 association.

    abstract::A majority of species B adenoviruses (Ads) use CD46 as their primary receptor; however, the precise mechanisms involved in the binding of different Ad types to CD46 have not been resolved. Although previous studies indicate close similarities between two members of species B2 Ads in their usage of CD46, our current in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00754-08

    authors: Pache L,Venkataraman S,Reddy VS,Nemerow GR

    更新日期:2008-08-01 00:00:00

  • Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus.

    abstract::Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.13.1.42-45.1974

    authors: Fritz RB

    更新日期:1974-01-01 00:00:00

  • Mapping the genomic location of the gene encoding alpha-amanitin resistance in vaccinia virus mutants.

    abstract::To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.57.1.65-70.1986

    authors: Villarreal EC,Hruby DE

    更新日期:1986-01-01 00:00:00

  • Activation of the Epstein-Barr virus replicative cycle by human herpesvirus 6.

    abstract::One common attribute of herpesviruses is the ability to establish latent, life-long infections. The role of virus-virus interaction in viral reactivation between or among herpesviruses has not been studied. Preliminary experiments in our laboratory had indicated that infection of Epstein-Barr virus (EBV) genome-positi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.11.6768-6777.1993

    authors: Flamand L,Stefanescu I,Ablashi DV,Menezes J

    更新日期:1993-11-01 00:00:00

  • Aerosol transmission of chronic wasting disease in white-tailed deer.

    abstract::While the facile transmission of chronic wasting disease (CWD) remains incompletely elucidated, studies in rodents suggest that exposure of the respiratory mucosa may be an efficient pathway. The present study was designed to address this question in the native cervid host. Here, we demonstrate aerosol transmission of...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02852-12

    authors: Denkers ND,Hayes-Klug J,Anderson KR,Seelig DM,Haley NJ,Dahmes SJ,Osborn DA,Miller KV,Warren RJ,Mathiason CK,Hoover EA

    更新日期:2013-02-01 00:00:00

  • A cyclic AMP-responsive DNA-binding protein (CREB2) is a cellular transactivator of the bovine leukemia virus long terminal repeat.

    abstract::To gain insight into the cellular regulation of bovine leukemia virus (BLV) trans activation, a lambda-gt11 cDNA library was constructed with mRNA isolated from a BLV-induced tumor and the recombinant proteins were screened with an oligonucleotide corresponding to the tax activation-responsive element (TAR). Two clone...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.2.766-772.1992

    authors: Willems L,Kettmann R,Chen G,Portetelle D,Burny A,Derse D

    更新日期:1992-02-01 00:00:00

  • Genome-based identification of cancer genes by proviral tagging in mouse retrovirus-induced T-cell lymphomas.

    abstract::The identification of tumor-inducing genes is a driving force for elucidating the molecular mechanisms underlying cancer. Many retroviruses induce tumors by insertion of viral DNA adjacent to cellular oncogenes, resulting in altered expression and/or structure of the encoded proteins. The availability of the mouse gen...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.3.2056-2062.2003

    authors: Kim R,Trubetskoy A,Suzuki T,Jenkins NA,Copeland NG,Lenz J

    更新日期:2003-02-01 00:00:00

  • Herpes simplex virus gD and virions accumulate in endosomes by mannose 6-phosphate-dependent and -independent mechanisms.

    abstract::Herpes simplex virus (HSV) glycoprotein D (gD) is modified with mannose 6-phosphate (M6P) and binds to M6P receptors (MPRs). MPRs are involved in the well-characterized pathway by which lysosomal enzymes are directed to lysosomes via a network of endosomal membranes. Based on the impaired ability of HSV to form plaque...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.4.3330-3339.1998

    authors: Brunetti CR,Dingwell KS,Wale C,Graham FL,Johnson DC

    更新日期:1998-04-01 00:00:00

  • Adaptive Mutations in Replicase Transmembrane Subunits Can Counteract Inhibition of Equine Arteritis Virus RNA Synthesis by Cyclophilin Inhibitors.

    abstract::Previously, the cyclophilin inhibitors cyclosporine (CsA) and alisporivir (ALV) were shown to inhibit the replication of diverse RNA viruses, including arteriviruses and coronaviruses, which both belong to the order Nidovirales In this study, we aimed to identify arterivirus proteins involved in the mode of action of ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00490-19

    authors: de Wilde AH,Boomaars-van der Zanden AL,de Jong AWM,Bárcena M,Snijder EJ,Posthuma CC

    更新日期:2019-08-28 00:00:00

  • Identification of a new neutralization antigenic site on poliovirus coat protein VP2.

    abstract::Major neutralization antigenic sites have been previously mapped by us on VP1, the largest capsid protein of poliovirus type 1. Here we report the first identification of the primary sequence of a neutralization antigenic site on capsid protein VP2. Inspection of the amino acid sequence of VP2 led to the selection and...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.52.2.719-721.1984

    authors: Emini EA,Jameson BA,Wimmer E

    更新日期:1984-11-01 00:00:00

  • High rate of genetic rearrangement during replication of a Moloney murine leukemia virus-based vector.

    abstract::A protocol was designed to measure the forward mutation rate over an entire gene replicated as part of a Moloney murine leukemia virus-based vector. For these studies, the herpes simplex virus thymidine kinase (tk) gene under the control of the spleen necrosis virus U3 promoter was used as target sequence since it all...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.11.6357-6364.1993

    authors: Varela-Echavarría A,Prorock CM,Ron Y,Dougherty JP

    更新日期:1993-11-01 00:00:00

  • Simian virus 40 small tumor antigen activates AKT and telomerase and induces anchorage-independent growth of human epithelial cells.

    abstract::Human keratinocytes immortalized by full-length or early-region simian virus 40 (SV40) DNA grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the E6/E7 genes of human papillomaviruses. To determine the molecular basis for this biological difference in growth, we have used the in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.21.10685-10691.2002

    authors: Yuan H,Veldman T,Rundell K,Schlegel R

    更新日期:2002-11-01 00:00:00