Abstract:
:The ICP18.5 gene (UL28) of herpes simplex virus type 1 is a member of a well-conserved gene family among herpesviruses and is thought to play a role in localization of viral glycoproteins. We have cloned, sequenced, and expressed the entire pseudorabies virus (PRV) ICP18.5 open reading frame in Escherichia coli as a Cro-ICP18.5 fusion protein. Rabbit antiserum against Cro-ICP18.5 immunoprecipitated a 79-kDa protein from PRV-infected cells as well as a 79-kDa protein from in vitro translation of a T7 RNA polymerase transcript of the ICP18.5 gene. ICP18.5 could be detected in infected cells by 2 h postinfection. Analysis by indirect immunofluorescence demonstrated that ICP18.5 became associated with the nucleus. Subcellular fractionation confirmed that ICP18.5 synthesized during a pulse-chase experiment appeared in the nuclear fraction with time and was stable for at least 2.5 h after synthesis. Pulse-chase analysis revealed that ICP18.5 was synthesized as a monomer during a 2-min pulse labeling but formed faster sedimenting complexes which were sensitive to sodium dodecyl sulfate (SDS) treatment. The majority of ICP18.5 appeared in complexes with an antigenically unrelated 70-kDa protein. Immunoblot analysis of total infected-cell extracts using polyvalent anti-ICP18.5 serum demonstrated that a 74-kDa cellular protein in addition to the 79-kDa ICP18.5 was detected. This cellular protein was present at similar levels in uninfected cells and in PRV-infected cells at least 12 h into the infectious cycle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pederson NE,Enquist LWdoi
10.1128/JVI.65.7.3746-3758.1991subject
Has Abstractpub_date
1991-07-01 00:00:00pages
3746-58issue
7eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::The phenomenon whereby the host immune system responds to only a few of the many possible epitopes in a foreign protein is termed immunodominance. Immunodominance occurs not only during microbial infection but also following vaccination, and clarification of the underlying mechanism may permit the rational design of v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4251-4259.2002
更新日期:2002-05-01 00:00:00
abstract::We tested whether the selection of target sites can be manipulated by fusing retroviral integrase with a sequence-specific DNA-binding protein. A hybrid protein that has the Escherichia coli LexA protein fused to the C terminus of the human immunodeficiency virus type 1 integrase was constructed. The fusion protein, I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.37-46.1996
更新日期:1996-01-01 00:00:00
abstract::Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the viri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.688-693.1969
更新日期:1969-11-01 00:00:00
abstract::The epithelium plays a key role in the spread of Lassa virus. Transmission from rodents to humans occurs mainly via inhalation or ingestion of droplets, dust, or food contaminated with rodent urine. Here, we investigated Lassa virus infection in cultured epithelial cells and subsequent release of progeny viruses. We s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02240-09
更新日期:2010-04-01 00:00:00
abstract::A double-isotopic label difference analysis of polyacrylamide gels has been used to distinguish between cellular and viral protein accumulation in infected cells and to quantify the kinetics of accumulation of viral polypeptides. This technique, coupled with the determination of total radioactive amino acid incorporat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.788-800.1974
更新日期:1974-04-01 00:00:00
abstract::The E7 proteins of human papillomaviruses (HPVs) promote S-phase reentry in differentiated keratinocytes of the squamous epithelia to support viral DNA amplification. In this study, we showed that nuclear p130 was present in the differentiated strata of several native squamous epithelia susceptible to HPV infection. I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01202-07
更新日期:2008-05-01 00:00:00
abstract::Precursor polyproteins containing translational products of the gag gene of Moloney murine leukemia virus were purified by gel electrophoresis and cleaved into large fragments by hydroxylamine, mild acid hydrolysis, or cyanogen bromide. The hydroxylamine cleavage method (specific for asparagine-glycine bonds) was adap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.841-859.1983
更新日期:1983-06-01 00:00:00
abstract::Mouse hepatitis virus (MHV) infection spreads from MHV-infected DBT cells, which express the MHV receptor CEACAM1 (MHVR), to BHK cells, which are devoid of the receptor, by intercellular membrane fusion (MHVR-independent fusion). This mode of infection is a property of wild-type (wt) JHMV cl-2 virus but is not seen in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.950-958.2002
更新日期:2002-02-01 00:00:00
abstract::Reovirus virions are nonenveloped icosahedral particles consisting of two concentric protein shells, termed outer capsid and core. Outer-capsid protein sigma1 is the viral attachment protein and binds carbohydrate molecules on the surface of host cells. Monoclonal antibody (MAb) 4F2, which is specific for outer-capsid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6625-6634.2001
更新日期:2001-07-01 00:00:00
abstract::The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.995-998.2004
更新日期:2004-01-01 00:00:00
abstract::Norovirus (NoV) infections are a significant health burden to society, yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02138-16
更新日期:2017-01-18 00:00:00
abstract::We investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (HSV-2). Unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin G (IgG) and secretory IgA (S-IgA) from immune secret...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8109-8115.1997
更新日期:1997-11-01 00:00:00
abstract:UNLABELLED:Ebola virus (EBOV) belongs to the group of nonsegmented negative-sense RNA viruses. The seven EBOV genes are separated by variable gene borders, including short (4- or 5-nucleotide) intergenic regions (IRs), a single long (144-nucleotide) IR, and gene overlaps, where the neighboring gene end and start signal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01863-14
更新日期:2014-11-01 00:00:00
abstract::The origin and role of the endonuclease activity associated with purified virions of simian virus 40, previously described by this and other laboratories, have been further investigated. We found that the enzymatic activity from virions of temperature-sensitive (ts) mutants is not more heat labile than that from wild-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.2.352-362.1976
更新日期:1976-02-01 00:00:00
abstract:UNLABELLED:The immunoglobulin superfamily protein receptors for poliovirus, human rhinovirus, and coxsackievirus B (CVB) serve to bind the viruses to target cells and to facilitate the release of the virus genome by catalyzing the transition from the mature infectious virus to the A-particle uncoating intermediate. Rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01790-14
更新日期:2014-10-01 00:00:00
abstract::Early sensing of viral components or infection-induced tissue damage is a prerequisite for the successful control of pathogenic viruses by the host innate immune system. Recent results from our laboratory show how immune cells use the DNA-sensing machinery to detect intracellular damage generated early during infectio...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01081-16
更新日期:2017-06-26 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) produces multiple mRNAs through differential splicing and polyadenylation. Reverse transcriptase PCR was used to characterize transcripts from exons 1, 2, 3, and 4 (immediate-early 1 [IE1]). The expected IE72 and IE19 mRNAs were detected, as well as ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8191-8200.2004
更新日期:2004-08-01 00:00:00
abstract::Poliovirus proteins, except P3-7c, are associated with the endoplasmic reticulum after extraction of the cytoplasm and centrifugation of membranes to equilibrium in sucrose gradients. Proteins P3-2, P2-X, and P3-9 are found preferentially among the rough endoplasmic reticulum, whereas P3-7c is located in smooth endopl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.777-783.1984
更新日期:1984-12-01 00:00:00
abstract::BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.30-39.1979
更新日期:1979-10-01 00:00:00
abstract::Monoclonal antibody vpg15 detects a 24-kDa cell surface protein on feline cells permissive for infection with feline immunodeficiency virus (FIV). The antibody blocks infection of FIV-susceptible cells, and expression of the vpg15 marker is decreased in FIV-infected cells in vitro. These results suggest that the antib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1667-1671.1993
更新日期:1993-03-01 00:00:00
abstract::RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.2.465-477.1976
更新日期:1976-11-01 00:00:00
abstract::A major determinant of neurovirulence for the GDVII strain of Theiler's virus, a murine picornavirus, was mapped to the P1 capsid protein region. Chimeric viruses were constructed by using sequences from the 5' noncoding and P1 regions of the virulent GDVII strain to replace equivalent regions of the less virulent BeA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3901-3907.1993
更新日期:1993-07-01 00:00:00
abstract:UNLABELLED:The ATR kinase has essential functions in maintenance of genome integrity in response to replication stress. ATR is recruited to RPA-coated single-stranded DNA at DNA damage sites via its interacting partner, ATRIP, which binds to the large subunit of RPA. ATR activation typically leads to activation of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01412-14
更新日期:2014-09-01 00:00:00
abstract::The limited efficiency of in vivo gene transfer by replication-deficient retroviral vectors remains an obstacle to achieving effective gene therapy for solid tumors. One approach to circumvent this problem is the use of replication-competent retroviral vectors. However, the application of such vectors is at a comparat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02470-06
更新日期:2007-07-01 00:00:00
abstract::Poliovirus (PV) modifies membrane-trafficking machinery in host cells for its viral RNA replication. To date, ARF1, ACBD3, BIG1/BIG2, GBF1, RTN3, and PI4KB have been identified as host factors of enterovirus (EV), including PV, involved in membrane traffic. In this study, we performed small interfering RNA (siRNA) scr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00114-12
更新日期:2012-05-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) B capsids are composed of seven proteins, designated VP5, VP19C, 21, 22a, VP23, VP24, and VP26 in order of decreasing molecular weight. Three proteins (21, 22a, and VP24) are encoded by a single open reading frame (ORF), UL26, and include a protease whose structure and function have...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5365-5374.1994
更新日期:1994-09-01 00:00:00
abstract::Amphiphilic fatty acid derivatives having a 14- to 20-carbon cis-unsaturated alkyl chain were found to be potent inhibitors of the entry process of the lipid-containing bacteriophage PR4. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.413-416.1979
更新日期:1979-01-01 00:00:00
abstract::Rhesus lymphocryptovirus (rLCV) and Epstein-Barr virus (EBV) are closely related gammaherpesviruses that infect and cause disease in rhesus monkeys and humans, respectively. Thus, rLCV is an important model system for EBV pathogenesis. Both rLCV and EBV express microRNAs (miRNAs), several conserved in sequence and gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00110-10
更新日期:2010-05-01 00:00:00
abstract::Influenza viruses unable to express NS1 protein (delNS1) replicate poorly and induce large amounts of interferon (IFN). They are therefore considered candidate viruses for live-attenuated influenza vaccines. Their attenuated replication is generally assumed to result from the inability to counter the antiviral host re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01725-12
更新日期:2012-11-01 00:00:00
abstract::The nucleotide and deduced amino acid sequences of the P1 region of the genomes of 10 independent equine rhinitis A virus (ERAV) isolates were determined and found to be very closely related. A panel of seven monoclonal antibodies to the prototype virus ERAV.393/76 that bound to nonneutralization epitopes conserved am...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10550-10556.2001
更新日期:2001-11-01 00:00:00