Abstract:
:We investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (HSV-2). Unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin G (IgG) and secretory IgA (S-IgA) from immune secretions were adjusted to their concentrations in vivo. Wild-type HSV-2 was incubated in the immunoglobulin preparations for 15 min in vitro, followed by inoculation into vaginae of nonimmune mice. HSV-2 was neutralized by unfractionated antibody and purified IgG from immune secretions but not by unfractionated nonimmune antibody or by purified immune S-IgA. The protective effect of IgG in vivo was investigated by passively transferring purified serum IgG from immune and nonimmune donors to nonimmune recipients before vaginal challenge infection. Immune IgG significantly reduced the percentage of vaginal epithelium infected, concentrations of shed virus protein in the vaginal lumen, and illness scores, even though the viral antibody titers in serum and vaginal secretions of recipient mice at the time of challenge were only 29 and 8%, respectively, of those in actively immunized mice. Additionally, removal of vaginal secretions from immune mice 10 min before vaginal challenge with HSV-2 significantly increased the concentration of shed virus protein in the vaginal lumen after challenge. Collectively, the data indicate that IgG antibody in vaginal secretions of immune mice provides early protection against vaginal challenge infection, probably by neutralizing virus in the vaginal lumen. In contrast, S-IgA antibody contributed relatively little to immune protection of the vagina.
journal_name
J Viroljournal_title
Journal of virologyauthors
Parr EL,Parr MBdoi
10.1128/JVI.71.11.8109-8115.1997subject
Has Abstractpub_date
1997-11-01 00:00:00pages
8109-15issue
11eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Reduction of major histocompatibility complex class I cell surface expression occurs in adenovirus-, herpes simplex virus-, human cytomegalovirus (HCMV)-, and murine cytomegalovirus-infected cell systems. Recently, it was demonstrated that the down-regulation mediated by HCMV infection is posttranslational, as a resul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4830-4841.1995
更新日期:1995-08-01 00:00:00
abstract::In most prion diseases, infectivity accumulates in lymphoreticular organs early after infection. Defects in hematopoietic compartments, such as impaired B-cell maturation, or in stromal compartments, such as abrogation of follicular dendritic cells, can delay or prevent lymphoreticular prion colonization. However, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7097-7106.2001
更新日期:2001-08-01 00:00:00
abstract::Natural evolution in primate lentiviral reverse transcriptase (RT) appears to have been constrained by the necessity to maintain function within an asymmetric protein composed of two identical primary amino acid sequences (66 kDa), of which one is cleaved (51 kDa). In this study, a detailed phylogenetic analysis now s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00991-10
更新日期:2010-10-01 00:00:00
abstract::The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6705-6711.1995
更新日期:1995-11-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5'-CAGGTA-3', called ZV, located at nucleotides -17 to -12 relative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.867-877.2001
更新日期:2001-01-01 00:00:00
abstract::ICP0 is a potent activator of herpes simplex virus type 1 gene expression in transient assays and in productive infection. A role for ICP0 in reactivation from latency in vivo has also been suggested on the basis of the observation that viruses with mutations in both copies of the diploid gene for ICP0 reactivate less...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7501-7512.1993
更新日期:1993-12-01 00:00:00
abstract::Numerous deaths of koi and common carp (Cyprinus carpio) were observed on many farms throughout Israel, resulting in severe financial losses. The lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. Diseased fish exhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.1983-1991.2005
更新日期:2005-02-01 00:00:00
abstract::Dengue virus (DENV) is an important human pathogen, especially in the tropical and subtropical parts of the world, causing considerable morbidity and mortality. DENV replication occurs in the cytoplasm; however, a high proportion of nonstructural protein 5 (NS5), containing methyltransferase (MTase) and RNA-dependent ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03083-12
更新日期:2013-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-06
更新日期:2006-09-01 00:00:00
abstract::As part of the continuing effort to develop an effective HIV vaccine, we generated a poxviral vaccine vector (previously described) designed to improve on the results of the RV144 phase III clinical trial. The construct, NYVAC-KC, is a replication-competent, attenuated recombinant of the vaccinia virus strain NYVAC. N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01513-18
更新日期:2019-01-17 00:00:00
abstract::To generate a null U(L)49 gene mutant of herpes simplex virus 1 (HSV-1), we deleted from the viral DNA, encoded as a bacterial artificial chromosome (BAC), the U(L)49 open reading frame and, in a second step, restored it. Upon transfection into Vero cells, the BAC-DeltaU(L)49 DNA yielded foci of degenerated cells that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01239-07
更新日期:2007-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5339-5345.1993
更新日期:1993-09-01 00:00:00
abstract::A/duck/Shanghai/28-1/2009(H4N2) (DK28) was isolated from a live poultry market in Shanghai, China. Using PCR and sequencing analysis, we obtained the complete genome sequences of the DK28 virus. The sequence analysis demonstrated that this H4N2 virus was a novel multiple-gene reassortant avian influenza virus (AIV) wh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02179-12
更新日期:2012-11-01 00:00:00
abstract::Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9137-9144.1999
更新日期:1999-11-01 00:00:00
abstract::The serological relationship of the ribonucleic acid (RNA)-containing coliphages MS-2, M-12, R-17, f(2), beta, fr, f(4), and Qbeta was determined. Antisera against MS-2, R-17, f(2), fr, and Qbeta neutralized the infectivity of all of these RNA phages to varying degrees. Although each phage was serologically distinct, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.5.567-573.1969
更新日期:1969-11-01 00:00:00
abstract::Type C RNA virus(es) was readily released from primary lymphosarcoma cell cultures of WH/J rabbits after induction with halogenated pyrimidines. The virus contained an RNA-directed DNA polymerase and the p30 structural protein. The rabbit virus RNA-directed DNA polymerase and p30 protein shared antigenic homologies wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.313-319.1978
更新日期:1978-08-01 00:00:00
abstract::Recombinant myxoma virus (MYXV) can be produced without a loss of infectivity, and its highly specific host range makes it an ideal vaccine vector candidate, although careful examination of its interaction with the immune system is necessary. Similar to rabbit bone marrow-derived dendritic cells (BM-DCs), ovine dendri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00128-11
更新日期:2011-12-01 00:00:00
abstract::The ribonucleoprotein (RNP) internal components of influenza virus were separated into distinct size classes by sedimentation in glycerol gradients and examined by electron microscopy by using positive staining with uranyl acetate. The large RNP have a peak in length distribution at 90 to 110 nm, the medium, at 60 to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.795-800.1972
更新日期:1972-10-01 00:00:00
abstract:UNLABELLED:Antibodies against the fusion (F) protein of respiratory syncytial virus (RSV) play an important role in the protective immune response to this important respiratory virus. Little is known, however, about antibody levels against multiple F-specific epitopes induced by infection or after vaccination against R...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-16
更新日期:2016-06-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.394-402.1984
更新日期:1984-02-01 00:00:00
abstract::Hepatitis C virus (HCV)-specific CD8 cell exhaustion may represent a mechanism of HCV persistence. The inhibitory receptor PD-1 has been reported to be up-regulated in exhausted CD8 cells. Therefore, we studied PD-1 expression longitudinally during acute HCV infection. Most HCV-specific CD8 cells expressed PD-1 at the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-06
更新日期:2006-11-01 00:00:00
abstract::Transcript levels from the P5 promoter of adeno-associated virus type 2 (AAV) are negatively regulated by the AAV Rep78 and Rep68 proteins in the absence of helper virus. We have identified a Rep-responsive negative cis element of the P5 promoter between the P5 TATA box and transcription start site by using 5' and 3' ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6787-6796.1995
更新日期:1995-11-01 00:00:00
abstract::The coronavirus, mouse hepatitis virus strain JHM, causes acute and chronic neurological diseases in rodents. Here we demonstrate that two closely related virus variants, both of which cause acute encephalitis in susceptible strains of mice, cause markedly different diseases if mice are protected with a suboptimal amo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10260-10269.2003
更新日期:2003-10-01 00:00:00
abstract::Human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. To gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. We show that for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6533-6540.2003
更新日期:2003-06-01 00:00:00
abstract::Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR viruses, we have engine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01397-13
更新日期:2013-10-01 00:00:00
abstract::We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.625-634.1981
更新日期:1981-12-01 00:00:00
abstract::To examine the pathway of the coreceptor switching of CCR5-using (R5) virus to CXCR4-using (X4) virus in simian-human immunodeficiency virus SHIV(SF162P3N)-infected rhesus macaque BR24, analysis was performed on variants present at 20 weeks postinfection, the time when the signature gp120 V3 loop sequence of the X4 sw...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-08
更新日期:2008-07-01 00:00:00
abstract::Human cytomegalovirus (HCMV) pathogenesis is dependent on the hematogenous spread of the virus to host tissue. While data suggest that infected monocytes are required for viral dissemination from the blood to the host organs, infected endothelial cells are also thought to contribute to this key step in viral pathogene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01016-06
更新日期:2006-12-01 00:00:00
abstract::Regulatory elements interacting with DNA-binding proteins have been investigated in the promoter sequence of the early PE38 gene in the Autographa californica nuclear polyhedrosis virus (AcNPV). A GATA motif located 50 nucleotides upstream of the PE38 transcriptional start site is recognized differentially in the cour...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3494-3503.1992
更新日期:1992-06-01 00:00:00
abstract::The functions of the two rotavirus glycoproteins were investigated by using tunicamycin and a variant of SA11 rotavirus having nonglycosylated VP7. Results showed that glycosylation of VP7 is not required for normal viral morphogenesis and infectivity and suggested that the nonstructural glycoprotein is involved in as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.270-274.1983
更新日期:1983-04-01 00:00:00