Abstract:
:Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use of other pathways. Here, we demonstrate using various inhibitors that functional entry of baculoviruses into insect and mammalian cells is primarily dependent on clathrin-mediated endocytosis. Our results further suggest that caveolae are somehow involved in baculovirus entry in mammalian cells. A caveolar endocytosis inhibitor, genistein, enhances baculovirus transduction in these cells considerably.
journal_name
J Viroljournal_title
Journal of virologyauthors
Long G,Pan X,Kormelink R,Vlak JMdoi
10.1128/JVI.00880-06subject
Has Abstractpub_date
2006-09-01 00:00:00pages
8830-3issue
17eissn
0022-538Xissn
1098-5514pii
80/17/8830journal_volume
80pub_type
杂志文章abstract::We have developed a high-throughput sequencing (HTS) workflow for investigating paramyxovirus transcription and replication. We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering the orientation of the RNAs from which they had been generated, cannot accurately be used to analyze the a...
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.66.10.6233-6236.1992
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4639-4650.1993
更新日期:1993-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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