Abstract:
:The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated in nearly every aspect of viral gene expression, including transcription, RNA stability, splicing, export, and translation. Here we demonstrate that ORF57 interacts with the KSHV K-bZIP protein in vitro and in cell extracts from lytically reactivated infected cells. To further test the biological relevance of the interaction, we performed a chromatin immunoprecipitation and microarray (ChIP-chip) analysis using anti-ORF57 antibodies and a KSHV tiling array. The results revealed four specific areas of enrichment, including the ORF4 and K8 (K-bZIP) promoters, as well as oriLyt, all of which interact with K-bZIP. In addition, ORF57 associated with DNA corresponding to the PAN RNA transcribed region, a known posttranscriptional target of ORF57. All of the peaks were RNase insensitive, demonstrating that ORF57 association with the viral genome is unlikely to be mediated exclusively by an RNA tether. Our data demonstrate that ORF57 associates with the viral genome by using at least two modes of recruitment, and they suggest that ORF57 and K-bZIP coregulate viral gene expression during lytic infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hunter OV,Sei E,Richardson RB,Conrad NKdoi
10.1128/JVI.03459-12subject
Has Abstractpub_date
2013-04-01 00:00:00pages
4005-16issue
7eissn
0022-538Xissn
1098-5514pii
JVI.03459-12journal_volume
87pub_type
杂志文章abstract::We have previously shown that the herpes simplex virus immediate-early regulatory protein ICP27 acts posttranscriptionally to affect mRNA processing (R. M. Sandri-Goldin and G. E. Mendoza, Genes Dev. 6:848-863, 1992). Specifically, in the presence of ICP27, spliced target mRNAs were decreased 5- to 10-fold in transfec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.4797-4810.1994
更新日期:1994-08-01 00:00:00
abstract::Influenza A virus NS2 protein, also called nuclear export protein (NEP), is crucial for the nuclear export of viral ribonucleoproteins. However, the molecular mechanisms of NEP mediation in this process remain incompletely understood. A leucine-rich nuclear export signal (NES2) in NEP, located at the predicted N2 heli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06519-11
更新日期:2013-01-01 00:00:00
abstract::Reverse transcription in hepatitis B viruses is initiated through a unique protein priming mechanism whereby the viral reverse transcriptase (RT) first assembles into a ribonucleoprotein (RNP) complex with its RNA template and then initiates DNA synthesis de novo using the RT itself as a protein primer. RNP formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11447-11455.2000
更新日期:2000-12-01 00:00:00
abstract::The highly syncytium-inducing M strain and the weakly syncytium-inducing SC strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (HN) and membrane (M) proteins, while their fusion (F) proteins are identical (T. Shioda, S. Wakao, S. Suzu, and H. Sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3661-3668.1989
更新日期:1989-09-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen affecting an estimated 3% of the world's population. Recent advances have enabled in vitro propagation of the virus and allow assembly and egress to be investigated for the first time. As a component of the virion, the HCV core protein likely functions primarily i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00793-07
更新日期:2007-10-01 00:00:00
abstract::Viral particles infecting some stocks of the protozoan parasite Leishmania braziliensis subsp. guyanensis contain a double-stranded RNA genome of ca. 5 kbp and are associated with an RNA-dependent RNA polymerase which synthesizes in vitro double-stranded and single-stranded, genome-length transcripts. The majority of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4211-4215.1991
更新日期:1991-08-01 00:00:00
abstract::Endocytosis and endosomal trafficking regulate the proteins targeted to the plasma membrane and play essential roles in diverse cellular processes, including responses to pathogen attack. Here, we report the identification of Glycine max (soybean) endocytosis dynamin-like protein 5A (GmSDL5A) associated with purified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01320-18
更新日期:2018-11-12 00:00:00
abstract::Herpesviruses encode an essential, maturational serine protease whose catalytic domain, assemblin (28 kDa), is released by self-cleavage from a 74-kDa precursor (pPR, pUL80a). Although there is considerable information about the structure and enzymatic characteristics of assemblin, a potential pharmacologic target, co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02821-06
更新日期:2007-04-01 00:00:00
abstract::In the course of our studies on oxidative stress as a component of pathological processes in humans, we showed that microintrusion into cells with microcapillary and ultramicroelectrochemical detection could mimic many types of mechanical intrusion leading to an instant (0.1 s) and high (some femtomoles) burst release...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1447-1452.1999
更新日期:1999-02-01 00:00:00
abstract::Kinetic curves for neutralization of adenovirus type 2 with anti-hexon serum revealed no lag periods even when the serum was highly diluted or when the temperature was lowered to 4 degrees C, thus indicating a single-hit mechanism. Multiplicity curves determined with anti-hexon serum displayed a linear correlation bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2321-2328.1988
更新日期:1988-07-01 00:00:00
abstract::We have obtained a polyclonal antiserum, N-BE, against the denatured, amino-terminal half of the measles virus (MV) nucleocapsid (N) protein and a monoclonal antibody (MAb), N46, which recognizes a conformation-dependent epitope in the same region. Amino acid residues 23 to 239 were required and sufficient for the for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4133-4141.1993
更新日期:1993-07-01 00:00:00
abstract::Mutants (N(-)nin) of bacteriophage lambda in which the N gene product is not required for growth on wild-type Escherichia coli do not plate on recA bacterial mutants. Secondary mutants, selected for growth on recA, lie within the immunity region to the right of gene cI and appear identical to the cro mutants of Eisen ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.938-945.1972
更新日期:1972-06-01 00:00:00
abstract::Adenovirus types 5 and 3 (Ad5 and Ad3), two human adenovirus serotypes of evolutionarily divergent subgroups, show very different levels of E1A gene expression early after infection of permissive cells. Since adenovirus E1A gene expression is known to be transcriptionally autoregulated, we have investigated the differ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1833-1838.1989
更新日期:1989-04-01 00:00:00
abstract::For efficient and versatile use of adenovirus (Ad) as an in vivo gene therapy vector, modulation of the viral tropism is highly desirable. In this study, a novel method to genetically alter the Ad fiber tropism is described. The knob and the last 15 shaft repeats of the fiber gene were deleted and replaced with an ext...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7280-7289.2001
更新日期:2001-08-01 00:00:00
abstract::Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a transmissible lung cancer of sheep known as ovine pulmonary carcinoma. Recently, we have found that the expression of the JSRV envelope (Env) is sufficient to transform mouse NIH 3T3 cells in classical transformation assays. To further investigate the mech...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.11002-11009.2001
更新日期:2001-11-01 00:00:00
abstract::HLA-B*57 is strongly associated with immune control of HIV and delayed AIDS progression. The closely related, but less protective, HLA-B*58:01 presents similar epitopes, but HLA-B*58:01(+) individuals do not generate CD8(+) T cells targeting the KF11-Gag epitope, which has been linked to low viremia. Here we show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01122-13
更新日期:2013-10-01 00:00:00
abstract::The capsid (CA) domain of the Moloney murine leukemia virus (Mo-MuLV) Gag protein has a unique carboxy terminus with a highly charged arginine-rich sequence. Mutant viruses harboring arginine-to-alanine mutations affecting this region of CA displayed significant defects in virion release, and the few viral particles p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3339-3344.2003
更新日期:2003-03-01 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequenci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3158-3167.2002
更新日期:2002-04-01 00:00:00
abstract::Nondefective reticuloendotheliosis virus induces chicken bursal lymphoma in a manner similar to that of avian leukosis virus. The provirus integrates in the c-myc locus and uses a promoter insertion mechanism to activate c-myc expression. We cloned a provirus involved in c-myc activation from a B lymphoma. Detailed st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2084-2090.1987
更新日期:1987-07-01 00:00:00
abstract::To investigate the genetic stability of vaccinia virus DNA, we have tested whether alterations occurred in the polypeptide composition of this complex virus during persistent infections. We found that variants isolated at various passages in Friend erythroleukemia cells persistently infected with vaccinia virus contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2642-2647.1987
更新日期:1987-08-01 00:00:00
abstract::Human coronavirus NL63 (HCoV-NL63), a common human respiratory pathogen, is associated with both upper and lower respiratory tract disease in children and adults. Currently, no antiviral drugs are available to treat CoV infections; thus, potential drug targets need to be identified and characterized. Here, we identify...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02747-06
更新日期:2007-06-01 00:00:00
abstract::Egress of wrapped virus (WV) to the cell periphery following vaccinia virus (VACV) replication is dependent on interactions with the microtubule motor complex kinesin-1 and is mediated by the viral envelope protein A36. Here we report that ectromelia virus (ECTV), a related orthopoxvirus and the causative agent of mou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06636-11
更新日期:2012-07-01 00:00:00
abstract::An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3307-3311.1997
更新日期:1997-04-01 00:00:00
abstract::Klebsiella pneumoniae is a member of the family Enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. The emergence of multidrug-resistant strains of K. pneumoniae has became a public health problem globally. To develop an effective antimicrobial agent, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02435-12
更新日期:2012-12-01 00:00:00
abstract::NYVAC has been engineered as a safe, attenuated vaccinia virus (VV) vector for use in vaccination against a broad spectrum of pathogens and tumors. Due to the interest in NYVAC-based vectors as vaccines and current phase I/II clinical trials with this vector, there is a need to analyze the human host response to NYVAC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.985-998.2006
更新日期:2006-01-01 00:00:00
abstract::Murine cytomegalovirus propagated in mouse embryo fibroblasts was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 x g for 90 min. (ii) The concentrated virus was passed through a Bio-Rad Bio-Gel A-15m column to eliminate contaminating materials smaller than 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.906-915.1976
更新日期:1976-03-01 00:00:00
abstract::BK polyomavirus (BKV) establishes persistent, low-level, and asymptomatic infections in most humans and causes polyomavirus-associated nephropathy (PVAN) and other pathologies in some individuals. The activation of BKV replication following kidney transplantation, leading to viruria, viremia, and, ultimately, PVAN, is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06369-11
更新日期:2012-03-01 00:00:00
abstract::We previously reported that CD4C/human immunodeficiency virus (HIV)(Nef) transgenic (Tg) mice, expressing Nef in CD4(+) T cells and cells of the macrophage/dendritic cell (DC) lineage, develop a severe AIDS-like disease, characterized by depletion of CD4(+) T cells, as well as lung, heart, and kidney diseases. In orde...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00125-09
更新日期:2009-10-01 00:00:00
abstract::Accumulation of the interferon-stimulated gene 15 (ISG15) protein product, which is reversibly conjugated to numerous polypeptide targets, impacts the proteome and physiology of uninfected and infected cells. While many viruses, including human cytomegalovirus (HCMV), blunt host antiviral defenses by limiting ISG expr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02483-16
更新日期:2017-04-13 00:00:00
abstract::Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00211-08
更新日期:2008-05-01 00:00:00