Abstract:
:We have obtained a polyclonal antiserum, N-BE, against the denatured, amino-terminal half of the measles virus (MV) nucleocapsid (N) protein and a monoclonal antibody (MAb), N46, which recognizes a conformation-dependent epitope in the same region. Amino acid residues 23 to 239 were required and sufficient for the formation of the conformational epitope. Using these antibodies, we show that the N protein of MV is synthesized as a relatively unfolded protein which first appears in the free-protein pool. This nascent N protein undergoes a conformational change into a more folded mature form. This change does not require the participation of other viral proteins or genomic RNA. The mature N protein does not accumulate in the free-protein pool but is quickly and selectively incorporated into the viral nucleocapsids. The mature N protein is a target for interaction with the phosphoprotein (P protein) of MV. This interaction interferes with the recognition of the N protein by the N46 MAb. This suggests that the association with the P protein may mask the binding site for the N46 MAb or that it induces a conformational change in the N protein.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gombart AF,Hirano A,Wong TCdoi
10.1128/JVI.67.7.4133-4141.1993subject
Has Abstractpub_date
1993-07-01 00:00:00pages
4133-41issue
7eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::Mother-to-child transmission can occur in utero, mainly intrapartum and postpartum in case of breastfeeding. In utero transmission is highly restricted and results in selection of viral variant from the mother to the child. We have developed an in vitro system that mimics the interaction between viruses, infected cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4780-4791.2001
更新日期:2001-05-01 00:00:00
abstract::We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was emp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01349-08
更新日期:2009-02-01 00:00:00
abstract::We previously reported the establishment of several lymphoid cell lines from X-ray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses (P. Sankar-Mistry and P. Jolicoeur, J. Virol.35:270-275, 1980). Biological characterization of five of these new primary radiation leukemia viruses (RadLVs) in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.565-575.1983
更新日期:1983-02-01 00:00:00
abstract::The fusion (F) protein of simian virus 5 (strain W3A) induced extensive cell fusion in BHK cells when expressed alone, while that of strain WR did not. Mutational analysis demonstrated that the fusing activity can be transferred to the WR F protein by a proline residue at position 22 of subunit W3A F2. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9855-9858.1997
更新日期:1997-12-01 00:00:00
abstract::The rotavirus nonstructural phosphoprotein NSP5 is encoded by a gene in RNA segment 11. Immunofluorescence analysis of fixed cells showed that NSP5 polypeptides remained confined to viroplasms even at a late stage when provirions migrated from these structures. When NSP5 was expressed in COS-7 cells in the absence of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6398-6405.1998
更新日期:1998-08-01 00:00:00
abstract::Callitrichid hepatitis is an infection of New World primates caused by an arenavirus, currently referred to as callitrichid hepatitis virus, that is closely related to lymphocytic choriomeningitis virus (LCMV). We have cloned and sequenced the GP-C gene of callitrichid hepatitis virus and found that the cDNA sequence ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1349-1352.1995
更新日期:1995-02-01 00:00:00
abstract::The complete nucleotide sequence of the NS mRNA of vesicular stomatitis virus (New Jersey serotype) was established from two cDNA clones spanning the entire coding region of the mRNA. The gene is 856 nucleotides long and can code for a polypeptide of 274 amino acids. Comparison with the nucleotide sequence of the NS g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.1.60-66.1985
更新日期:1985-07-01 00:00:00
abstract::Adenovirus (Ad) gene transfer vectors can be used to transfer and express antigens and function as strong adjuvants and thus are useful platforms for the development of genetic vaccines. Based on the hypothesis that Ad vectors with enhanced infectibility of dendritic cells (DC) may be able to evoke enhanced immune res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2572-2580.2004
更新日期:2004-03-01 00:00:00
abstract::The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00056-10
更新日期:2010-07-01 00:00:00
abstract::Binding of the human immunodeficiency virus type 1 (HIV-1) Gag protein precursor, Pr55(Gag), to membrane is an indispensable step in virus assembly. Previously, we reported that a matrix (MA) residue 6 substitution (6VR) imposed a virus assembly defect similar to that observed with myristylation-defective mutants, sug...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4136-4144.1999
更新日期:1999-05-01 00:00:00
abstract::Retroviruses integrate into cellular DNA nonrandomly. Lentiviruses such as human immunodeficiency virus type 1 (HIV-1) favor the bodies of active genes and gene-enriched transcriptionally active regions of chromosomes. The interaction between lentiviral integrase and the cellular protein lens epithelium-derived growth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01148-12
更新日期:2013-01-01 00:00:00
abstract::Tick-borne encephalitis virus (TBEV), a major tick-borne viral pathogen of humans, is known to cause neurological diseases such as meningitis, encephalitis, and meningoencephalitis. However, the life cycle and pathogenesis of TBEV are not well understood. Here, we show that the knockdown or knockout of ADAM15 (a disin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01926-20
更新日期:2021-01-28 00:00:00
abstract::Myxomatosis is a recurrent problem on rabbit farms throughout Europe despite the success of vaccines. To identify gene variations of field and vaccine strains that may be responsible for changes in virulence, immunomodulation, and immunoprotection, the genomes of 6 myxoma virus (MYXV) strains were sequenced: German fi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01570-16
更新日期:2017-01-31 00:00:00
abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
abstract::Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3330-3338.1992
更新日期:1992-06-01 00:00:00
abstract::A selection strategy, the activator trap, was used in order to identify genes of human cytomegalovirus (HCMV) that encode strong transcriptional activation domains in mammalian cells. This approach is based on the isolation of activation domains from a GAL4 fusion library by means of selective plasmid replication, whi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4836-4847.2002
更新日期:2002-05-01 00:00:00
abstract::Recombinant vaccinia viruses expressing C-terminally truncated E's that ranged in length from 9 to 99% of the N-terminal sequence were constructed. The overall antigenicity of the E products was analyzed by radioimmunoprecipitation, using dengue virus hyperimmune mouse ascitic fluid (HMAF) or an anti-E peptide serum. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1400-1407.1991
更新日期:1991-03-01 00:00:00
abstract::The infection of a bacterium by a phage starts with attachment to a receptor molecule on the host cell surface by the phage. Since receptor-phage interactions are crucial to successful infections, they are major determinants of phage host range and, by extension, of the broader effects that phages have on bacterial co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-20
更新日期:2020-11-09 00:00:00
abstract::PVC-211 murine leukemia virus (MuLV) is a neuropathogenic retrovirus that has undergone genetic changes from its nonneuropathogenic parent, Friend MuLV, that allow it to efficiently infect rat brain capillary endothelial cells (BCEC). To clarify the mechanism by which PVC-211 MuLV expression in BCEC induces neurologic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02343-08
更新日期:2009-05-01 00:00:00
abstract::Fatal infection with the highly pathogenic Lassa virus (LASV) is characterized by extensive viral dissemination, indicating broad tissue tropism. The major cellular receptor for LASV is the highly conserved extracellular matrix receptor dystroglycan (DG). Binding of LASV depends on DG's tissue-specific posttranslation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01613-17
更新日期:2018-02-12 00:00:00
abstract::The nucleotide sequences of several sites of recombination between adenovirus DNA and hamster, mouse, or human cell DNAs were determined. These sites of recombination had been cloned from adenovirus type 2 (Ad2)- or type 12 (Ad12)-transformed cells, from Ad12-induced tumor cells, or from a symmetric recombinant betwee...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.344-353.1987
更新日期:1987-02-01 00:00:00
abstract::Humans differ substantially with respect to susceptibility to human immunodeficiency virus type 1 (HIV-1). We evaluated variants of nine host genes participating in the viral life cycle for their role in modulating HIV-1 infection. Alleles were assessed ex vivo for their impact on viral replication in purified CD4 T c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12674-12680.2005
更新日期:2005-10-01 00:00:00
abstract::Coronaviruses generally have a narrow host range, infecting one or just a few species. Using targeted RNA recombination, we constructed a mutant of the coronavirus mouse hepatitis virus (MHV) in which the ectodomain of the spike glycoprotein (S) was replaced with the highly divergent ectodomain of the S protein of fel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1393-1406.2000
更新日期:2000-02-01 00:00:00
abstract::A vaccine regimen that elicits broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine research. In this study, we assessed the immunogenicity of the CAP256 superinfecting viral envelope (CAP256 SU) protein delivered by modified vaccinia virus Ankara (MVA) and DNA vaccines in different prime-boost com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
更新日期:2019-04-03 00:00:00
abstract::Activation of IκB kinase subunit γ (IKKγ), a key regulator of the classical NF-κB pathway, by the vFLIP protein of Kaposi's sarcoma-associated herpesvirus (KSHV) and the Tax protein of human T cell lymphotropic virus type 1 (HTLV1) is essential for virus-associated cancer. We show that vFLIP and Tax activate this path...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02337-10
更新日期:2011-07-01 00:00:00
abstract::The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a formidable battle horse for the study of viral immunology, as well as viral persistence and associated diseases. Investigations with LCMV have uncovered basic mechanisms by which viruses avoid elimination by the host adaptive immune response. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00555-06
更新日期:2006-09-01 00:00:00
abstract::Hepadnaviruses contain a relaxed circular DNA genome (RC-DNA) with discontinuities in both strands. Upon infectious entry into a host cell, this genome is converted into a covalently closed superhelical form (CCC-DNA), which later serves as the template for transcription. Here we examined whether the viral polymerase ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4867-4874.1993
更新日期:1993-08-01 00:00:00
abstract::Y-box binding protein YB-1 is a member of a family of DNA and RNA binding proteins which have been shown to affect gene expression at both the transcriptional and translational levels. We have previously shown that YB-1 modulates transcription from the promoters of the ubiquitous human polyomavirus JC virus (JCV). Her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10146-10157.1999
更新日期:1999-12-01 00:00:00
abstract::S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.221-224.1987
更新日期:1987-01-01 00:00:00
abstract::Two major DNA variants have been isolated from serially propagated stocks of the WR strain of vaccinia virus. Restriction enzyme mapping of the two variants with HindIII, AvaI, XhoI, SstII, and SmaI revealed a 6.3-megadalton deletion in the smaller DNA variant. The deletion was mapped at ca. 6.8 megadaltons in from th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1000-1010.1981
更新日期:1981-03-01 00:00:00