Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier.

abstract：:Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mi...

journal_title：Journal of virology

authors： Fritz RB

更新日期：1974-01-01 00:00:00

abstract：:Infectious leukemia virus production by two chronically infected NIH/MOL lines was strongly inhibited by interferon treatment of the cells. The corresponding degree of inhibition in JLSV-11 cells was much lower. Multiplication of encephalomyocarditis virus in all three cell lines was barely affected by interferon trea...

journal_title：Journal of virology

authors： Sen GC,Herz RE

更新日期：1983-03-01 00:00:00

abstract：:In an attempt to generate broadly cross-reactive, neutralizing monoclonal antibodies (MAbs) to simian immunodeficiency virus (SIV), we compared two immunization protocols using different preparations of oligomeric SIV envelope (Env) glycoproteins. In the first protocol, mice were immunized with soluble gp140 (sgp140) ...

journal_title：Journal of virology

authors： Edinger AL,Ahuja M,Sung T,Baxter KC,Haggarty B,Doms RW,Hoxie JA

更新日期：2000-09-01 00:00:00

abstract：:Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter--abse...

journal_title：Journal of virology

authors： Leahy MB,Zecchin G,Brownlee GG

更新日期：2002-02-01 00:00:00

abstract：:The G glycoprotein of human respiratory syncytial virus (RSV) was identified previously as the viral attachment protein. Although we and others recently showed that G is not essential for replication in vitro, it does affect the efficiency of replication in a cell type-dependent fashion and is required for efficient r...

journal_title：Journal of virology

authors： Teng MN,Collins PL

更新日期：2002-06-01 00:00:00

abstract：:The effect of bacteriophage SPO1 infection of Bacillus subtilis and a deoxyribonucleic acid (DNA) polymerase-deficient (pol(-)) mutant of this microorganism on the synthesis of DNA has been examined. Soon after infection, the incorporation of deoxyribonucleoside triphosphates into acid-insoluble material by cell lysat...

journal_title：Journal of virology

authors： Yehle CO,Ganesan AT

更新日期：1972-02-01 00:00:00

abstract：:The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protei...

journal_title：Journal of virology

authors： Marín-López A,Calvo-Pinilla E,Barriales D,Lorenzo G,Brun A,Anguita J,Ortego J

更新日期：2018-07-31 00:00:00

abstract：:Upon stimulation with alpha/beta interferon, rat cells synthesize three Mx proteins. Sequence analysis of corresponding cDNAs reveals that these three proteins are derived from three distinct genes. One of the rat cDNAs is termed Mx1 because it is most closely related to the mouse Mx1 cDNA and because it codes for a n...

journal_title：Journal of virology

authors： Meier E,Kunz G,Haller O,Arnheiter H

更新日期：1990-12-01 00:00:00

abstract：:The genetic evolution of human immunodeficiency virus type 1 (HIV-1) in the brain is distinct from that in lymphoid tissues, indicating tissue-specific compartmentalization of the virus. Few primary HIV-1 envelope glycoproteins (Envs) from uncultured brain tissues have been biologically well characterized. In this stu...

journal_title：Journal of virology

authors： Ohagen A,Devitt A,Kunstman KJ,Gorry PR,Rose PP,Korber B,Taylor J,Levy R,Murphy RL,Wolinsky SM,Gabuzda D

更新日期：2003-11-01 00:00:00

abstract：:The binding specificities of a panel of avian influenza virus subtype H5 hemagglutinin (HA) proteins bearing mutations at key residues in the receptor binding site were investigated. The results demonstrate that two simultaneous mutations in the receptor binding site resulted in H5 HA binding in a pattern similar to t...

journal_title：Journal of virology

authors： Harvey R,Martin AC,Zambon M,Barclay WS

更新日期：2004-01-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection leads to lifelong viral persistence through its latency in B cells. EBV-specific T cells control reactivations and prevent the development of EBV-associated malignancies in most healthy carriers, but infection can sometimes cause chronic disease and malignant transformation. Epstein-...

journal_title：Journal of virology

authors： Leskowitz RM,Zhou XY,Villinger F,Fogg MH,Kaur A,Lieberman PM,Wang F,Ertl HC

更新日期：2013-08-01 00:00:00

abstract：:Two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of T4D and other T-even bacteriophage particles. The two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding...

journal_title：Journal of virology

authors： Kozloff LM,Lute M,Crosby LK

更新日期：1975-12-01 00:00:00

abstract：:MC159 is a viral FLIP (FLICE inhibitory protein) encoded by the molluscum contagiosum virus (MCV) enabling MCV to evade antiviral immunity and to establish persistent infections in humans. Here, we show that MC159 contains a functional SH3 binding motif, which mediates avid and selective binding to SH3BP4, a signaling...

journal_title：Journal of virology

authors： Schmotz C,Uğurlu H,Vilen S,Shrestha S,Fagerlund R,Saksela K

更新日期：2019-05-01 00:00:00

abstract：:Experimental evidence from the simian immunodeficiency virus (SIV) model of AIDS has shown that the nef gene is critical in the pathogenesis of AIDS. Consequently, nef is of considerable interest in both antiviral drug and vaccine development. Preliminary findings in two rhesus macaques indicated that a deletion of on...

journal_title：Journal of virology

authors： Whatmore AM,Cook N,Hall GA,Sharpe S,Rud EW,Cranage MP

更新日期：1995-08-01 00:00:00

abstract：:We used 18 monoclonal antibodies against B19 parvovirus to identify neutralizing epitopes on the viral capsid. Of the 18 antibodies, 9 had in vitro neutralizing activity in a bone marrow colony culture assay. The overlapping polypeptide fragments spanning the B19 structural proteins were produced in a pMAL-c Escherich...

journal_title：Journal of virology

authors： Yoshimoto K,Rosenfeld S,Frickhofen N,Kennedy D,Hills R,Kajigaya S,Young NS

更新日期：1991-12-01 00:00:00

abstract：:To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...

journal_title：Journal of virology

authors： von der Weth A,Deppert W

更新日期：1993-02-01 00:00:00

abstract：:In order to elucidate the molecular mechanisms of action of the tat-responsive sequence, mutational analysis of the tat-responsive sequence was carried out. The most critical region comprised nucleotides +18 to +44 and included the 3-nucleotide bulge at positions +23 to +25, the loop sequence, and an intact stem. In a...

journal_title：Journal of virology

authors： Roy S,Parkin NT,Rosen C,Itovitch J,Sonenberg N

更新日期：1990-03-01 00:00:00

abstract：:Heating oncornavirus RNAs at temperatures insufficient for complete denaturation results in forms migrating between the native form (vRNA) and the completely denatured form (vRNA) after gel electrophoresis. Intermediate forms from Rous sarcoma virus or murine leukemia virus were isolated after heating of vRNA's at 58 ...

journal_title：Journal of virology

authors： Bader JP,Ray DA

更新日期：1976-09-01 00:00:00

abstract：:Heron hepatitis B virus (HHBV) is an avian hepadnavirus that is closely related to duck hepatitis B virus (DHBV). To learn more about the mechanism of hepadnavirus replication, we characterized a clone of HHBV that contains a substitution of DHBV sequence from nucleotide coordinates 403 to 1364. This clone, named HDE1...

journal_title：Journal of virology

authors： Mueller-Hill K,Loeb DD

更新日期：1996-12-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) protease and its substrate, the assembly protein ICP35, are involved in virion maturation. Both proteins are encoded by a single open reading frame but are translated independently from 3'-coterminal mRNAs of different sizes and are in frame. The herpesvirus shell assembles arou...

journal_title：Journal of virology

authors： Robertson BJ,McCann PJ 3rd,Matusick-Kumar L,Preston VG,Gao M

更新日期：1997-02-01 00:00:00

abstract：:PEPSCAN analysis has been used to characterize the immunogenic regions of the capsid protein (CP) in virions of plum pox potyvirus (PPV). In addition to the well-known highly immunogenic N- and C-terminal domains of CP, regions within the core domain of the protein have also shown high immunogenicity. Moreover, the N ...

journal_title：Journal of virology

authors： Fernández-Fernández MR,Martínez-Torrecuadrada JL,Roncal F,Domínguez E,García JA

更新日期：2002-12-01 00:00:00

abstract：:Macrophage adherence, an important regulatory signal, has the potential to affect human immunodeficiency virus (HIV) production either directly or by priming monocytes to respond to other activating signals. We have investigated the role of adherence as an activator of HIV-1 transcription and release. The effects of a...

journal_title：Journal of virology

authors： Shattock RJ,Friedland JS,Griffin GE

更新日期：1993-06-01 00:00:00

abstract：:Little is known about the biological significance of human adenovirus type 5 (Ad5) E1A in vivo. However, Ad5 E1A is well defined in vitro and can be detected frequently in the lungs of patients with pulmonary disease. Transgenic expression of the Ad5 E1A gene targeted to the mouse lung reveals distinct biological effe...

journal_title：Journal of virology

authors： Yang Y,McKerlie C,Borenstein SH,Lu Z,Schito M,Chamberlain JW,Buchwald M

更新日期：2002-09-01 00:00:00

abstract：:To examine the mode of transmission of BK polyomavirus (BKV), urine samples were collected from Japanese-Americans in Los Angeles and from other southern Californians. Subtype I was the main subtype found in samples from both groups. The subtype I subgroup Ib-2, which is predominant in Europe, was the primary subgroup...

journal_title：Journal of virology

authors： Yogo Y,Zhong S,Suzuki M,Shibuya A,Kitamura T

更新日期：2007-12-01 00:00:00

abstract：:Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...

journal_title：Journal of virology

authors： Han Q,Aligo J,Manna D,Belton K,Chintapalli SV,Hong Y,Patterson RL,van Rossum DB,Konan KV

更新日期：2011-07-01 00:00:00

abstract：:The protein product of varicella-zoster virus (VZV) ORF47 is a serine/threonine protein kinase and tegument component. Evaluation of two recombinants of the Oka strain, rOka47DeltaC, with a C-terminal truncation of ORF47, and rOka47D-N, with a point mutation in the conserved kinase motif, showed that ORF47 kinase func...

journal_title：Journal of virology

authors： Besser J,Ikoma M,Fabel K,Sommer MH,Zerboni L,Grose C,Arvin AM

更新日期：2004-12-01 00:00:00

abstract：:We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...

journal_title：Journal of virology

authors： Lee PS,Zhu X,Yu W,Wilson IA

更新日期：2015-07-01 00:00:00

abstract：:Rift Valley fever (RVF) virus (RVFV) can cause severe human disease characterized by either acute-onset hepatitis, delayed-onset encephalitis, retinitis and blindness, or a hemorrhagic syndrome. The existing nonhuman primate (NHP) model for RVF utilizes an intravenous (i.v.) exposure route in rhesus macaques (Macaca m...

journal_title：Journal of virology

authors： Smith DR,Bird BH,Lewis B,Johnston SC,McCarthy S,Keeney A,Botto M,Donnelly G,Shamblin J,Albariño CG,Nichol ST,Hensley LE

更新日期：2012-02-01 00:00:00

abstract：:Dendritic cells (DCs) play a central role in instructing antiviral immune responses. DCs, however, can become targeted by different viruses themselves. We recently demonstrated that human DCs can be productively infected with echoviruses (EVs), but not coxsackie B viruses (CVBs), both of which are RNA viruses belongin...

journal_title：Journal of virology

authors： Kramer M,Schulte BM,Toonen LW,Barral PM,Fisher PB,Lanke KH,Galama JM,van Kuppeveld FJ,Adema GJ

更新日期：2008-03-01 00:00:00

abstract：:The structural instability of inactivated foot-and-mouth disease virus (FMDV) hinders the development of vaccine industry. Here we found that some transition metal ions like Cu2+ and Ni2+ could specifically bind to FMDV capsids at capacities about 7089 and 3448 metal ions per capsid, respectively. These values are abo...

journal_title：Journal of virology

authors： Lin X,Yang Y,Song Y,Li S,Zhang X,Su Z,Zhang S

更新日期：2021-01-13 00:00:00