The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo.

abstract：:RNA silencing is a potent means of antiviral defense in plants and animals. A hallmark of this defense response is the production of 21- to 24-nucleotide viral small RNAs via mechanisms that remain to be fully understood. Many viruses encode suppressors of RNA silencing, and some viral RNAs function directly as silenc...

journal_title：Journal of virology

authors： Itaya A,Zhong X,Bundschuh R,Qi Y,Wang Y,Takeda R,Harris AR,Molina C,Nelson RS,Ding B

更新日期：2007-03-01 00:00:00

abstract：:We applied deep sequencing technology to small RNA fractions from cells lytically infected with murine gammaherpesvirus 68 (gammaHV68) in order to define in detail small RNAs generated from a cluster of tRNA-related polycistronic structures located at the left end of the viral genome. We detected 10 new candidate micr...

journal_title：Journal of virology

authors： Reese TA,Xia J,Johnson LS,Zhou X,Zhang W,Virgin HW

更新日期：2010-10-01 00:00:00

abstract：:Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...

journal_title：Journal of virology

authors： Hellen CU,Lee CK,Wimmer E

更新日期：1992-06-01 00:00:00

abstract：:We have analyzed the production of tumor necrosis factor alpha (TNF-alpha) induced by in vitro infection with African swine fever (ASF) virus (ASFV) and the systemic and local release of this inflammatory cytokine upon in vivo infection. An early increase in TNF-alpha mRNA expression was detected in ASFV-infected alve...

journal_title：Journal of virology

authors： Gómez del Moral M,Ortuño E,Fernández-Zapatero P,Alonso F,Alonso C,Ezquerra A,Domínguez J

更新日期：1999-03-01 00:00:00

abstract：:Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a s...

journal_title：Journal of virology

authors： Summers MD,Anderson DL

更新日期：1972-04-01 00:00:00

abstract：:Infection by rat virus has been studied in cultures of rat embryo cells to evaluate the Margolis-Kilham hypothesis that the virus preferentially infects tissues with actively dividing cells. An enhancement of infection was seen in cultures infected 10 hr after fresh medium was added as compared to infection of station...

journal_title：Journal of virology

authors： Tennant RW,Layman KR,Hand RE

更新日期：1969-12-01 00:00:00

abstract：:The vesicular stomatitis virus (VSV) RNA-dependent RNA polymerase consists of two viral proteins; the large (L) protein is the main catalytic subunit, and the phosphoprotein (P) is an essential cofactor for polymerase function. The P protein interacts with the L protein and the N-RNA template, thus connecting the poly...

journal_title：Journal of virology

authors： Mondal A,Victor KG,Pudupakam RS,Lyons CE,Wertz GW

更新日期：2014-02-01 00:00:00

abstract：:T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...

journal_title：Journal of virology

authors： Yamada Y,Nakada D

更新日期：1976-06-01 00:00:00

abstract：:Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...

journal_title：Journal of virology

authors： Després P,Griffin JW,Griffin DE

更新日期：1995-11-01 00:00:00

abstract：:The human T-cell leukemia virus type 1 (HTLV-1) envelope protein is required for virus spread. This study further characterizes the role of the envelope protein in HTLV-1 immortalization. Viruses with single amino acid substitutions within the SU protein at residue 75, 81, 95, 101, 105, or 195 or with a C-terminal cyt...

journal_title：Journal of virology

authors： Tsukahara T,Wielgosz MM,Ratner L

更新日期：2001-10-01 00:00:00

abstract：:Lentiviruses have in their transmembrane glycoprotein (TM) a highly immunogenic structure referred to as the principal immunodominant domain (PID). The PID forms a loop of 5 to 7 amino acids between two conserved cysteines. Previous studies showed that envelope (Env) glycoprotein functions of feline immunodeficiency v...

journal_title：Journal of virology

authors： Merat R,Raoul H,Leste-Lasserre T,Sonigo P,Pancino G

更新日期：1999-07-01 00:00:00

abstract：:Group I host range (hr) mutants of adenovirus type 5 are unable to transform rat embryo or rat embryo brain cells but induce an abnormal transformation of baby rat kidney cells. We established several transformed rat kidney cell lines and characterized them with respect to the transformed phenotype and the structure o...

journal_title：Journal of virology

authors： Ruben M,Bacchetti S,Graham FL

更新日期：1982-02-01 00:00:00

abstract：:The human papillomavirus (HPV) minor capsid protein L2 plays important roles in the generation of infectious viral particles and in the initial steps of infection. Here we show that HPV-16 L2 protein is sumoylated at lysine 35 and that sumoylation affects its stability. Interestingly, the sumoylated form of L2 cannot ...

journal_title：Journal of virology

authors： Marusic MB,Mencin N,Licen M,Banks L,Grm HS

更新日期：2010-11-01 00:00:00

abstract：:In varicella-zoster virus (VZV)-infected primary human brain vascular adventitial fibroblasts (BRAFs), levels of beta interferon (IFN-β,) STAT1, and STAT2 transcripts as well as STAT1 and STAT2 protein were decreased. IFN-α transcript levels were increased but not secreted IFN-α protein levels. Compared to IFN-α-treat...

journal_title：Journal of virology

authors： Nagel MA,James SF,Traktinskiy I,Wyborny A,Choe A,Rempel A,Baird NL,Gilden D

更新日期：2014-10-01 00:00:00

abstract：:New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorptio...

journal_title：Journal of virology

authors： Reimer CB,Baker RS,Van Frank RM,Newlin TE,Cline GB,Anderson NG

更新日期：1967-12-01 00:00:00

abstract：:The L5R gene of vaccinia virus is conserved among all sequenced members of the Poxviridae but has no predicted function or recognized nonpoxvirus homolog. Here we provide the initial characterization of the L5 protein. L5 is expressed following DNA replication with kinetics typical of a viral late protein, contains a ...

journal_title：Journal of virology

authors： Townsley AC,Senkevich TG,Moss B

更新日期：2005-09-01 00:00:00

abstract：:Viruses are considered to use vesicular trafficking in infected cells, but the details of assembly/release pathways of hepatitis B virus (HBV) are still unknown. To identify key regulators of HBV production, we performed short interfering RNA (siRNA) screening for Rab proteins, which are considered to act as molecular...

journal_title：Journal of virology

authors： Inoue J,Ninomiya M,Umetsu T,Nakamura T,Kogure T,Kakazu E,Iwata T,Takai S,Sano A,Fukuda M,Watashi K,Isogawa M,Tanaka Y,Shimosegawa T,McNiven MA,Masamune A

更新日期：2019-07-17 00:00:00

abstract：:The infectious entry pathway of duck hepatitis B virus (DHBV) was investigated with primary duck hepatocytes. Virus uptake was measured by a selective PCR technique which allows for the detection of a successful infection without the need for viral replication or gene expression. To test whether DHBV uptake occurs by ...

journal_title：Journal of virology

authors： Köck J,Borst EM,Schlicht HJ

更新日期：1996-09-01 00:00:00

abstract：:Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding...

journal_title：Journal of virology

authors： Freel SA,Lamoreaux L,Chattopadhyay PK,Saunders K,Zarkowsky D,Overman RG,Ochsenbauer C,Edmonds TG,Kappes JC,Cunningham CK,Denny TN,Weinhold KJ,Ferrari G,Haynes BF,Koup RA,Graham BS,Roederer M,Tomaras GD

更新日期：2010-05-01 00:00:00

abstract：:The S2 gene of bluetongue virus, serotype 17, has been cloned, and the nonstructural protein NS2 has been expressed. Synthetic peptides matching regions within the amino acid sequence of NS2 were used to map three single-stranded RNA (ssRNA)-binding regions within the protein. A prokaryotic expression system was then ...

journal_title：Journal of virology

authors： Fillmore GC,Lin H,Li JK

更新日期：2002-01-01 00:00:00

abstract：:Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs...

journal_title：Journal of virology

authors： Conely AJ,Velicer LF

更新日期：1978-03-01 00:00:00

abstract：:Apoptosis of neurons and astrocytes is induced by human immunodeficiency type 1 (HIV-1) infection in vitro and has been demonstrated in brain tissue from patients with AIDS. We analyzed a panel of diverse HIV-1 primary isolates for the ability to replicate and induce neuronal and astrocyte apoptosis in primary human b...

journal_title：Journal of virology

authors： Ohagen A,Ghosh S,He J,Huang K,Chen Y,Yuan M,Osathanondh R,Gartner S,Shi B,Shaw G,Gabuzda D

更新日期：1999-02-01 00:00:00

abstract：:Extracts of competent cells of Bacillus subtilis exhibited nuclease activity on radioactively labeled cell deoxyribonucleic acid (DNA). The activity was not decreased when Mg was omitted from the reaction mixture but was decreased to zero by addition of ethylenediaminetetraacetic acid. Of the other metals tested, only...

journal_title：Journal of virology

authors： Epstein HT

更新日期：1971-06-01 00:00:00

abstract：:A number of options are available to modify and improve DNA vaccines. An interesting approach to improve DNA vaccines is to fuse bioactive domains, like cytotoxic-T-lymphocyte-associated protein 4 (CTLA-4), to an antigen. Such fusion antigens are expressed in vivo and directed to immune cells by the specific bioactive...

journal_title：Journal of virology

authors： Lu M,Isogawa M,Xu Y,Hilken G

更新日期：2005-05-01 00:00:00

abstract：:Activation of IκB kinase subunit γ (IKKγ), a key regulator of the classical NF-κB pathway, by the vFLIP protein of Kaposi's sarcoma-associated herpesvirus (KSHV) and the Tax protein of human T cell lymphotropic virus type 1 (HTLV1) is essential for virus-associated cancer. We show that vFLIP and Tax activate this path...

journal_title：Journal of virology

authors： Shimizu A,Baratchian M,Takeuchi Y,Escors D,Macdonald D,Barrett T,Bagneris C,Collins M,Noursadeghi M

更新日期：2011-07-01 00:00:00

abstract：:The hepatitis C virus (HCV)-specific CD4+ T-cell response against nonstructural proteins is strongly associated with successful viral clearance during acute hepatitis C. To further develop these observations into peptide-based vaccines and clinical immunomonitoring tools like HLA class II tetramers, a detailed charact...

journal_title：Journal of virology

authors： Gerlach JT,Ulsenheimer A,Grüner NH,Jung MC,Schraut W,Schirren CA,Heeg M,Scholz S,Witter K,Zahn R,Vogler A,Zachoval R,Pape GR,Diepolder HM

更新日期：2005-10-01 00:00:00

abstract：:Proviral clones of human immunodeficiency virus type 1 which contained single amino acid changes in the envelope V3 region were constructed. PCR amplification of Sup-T1 T cells transfected with one such mutant, G312T, revealed low levels of virus that resulted in the generation of a revertant virus, in which an alanin...

journal_title：Journal of virology

authors： Morris JF,Sternberg EJ,Gutshall L,Petteway SR Jr,Ivanoff LA

更新日期：1994-12-01 00:00:00

abstract：:Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...

journal_title：Journal of virology

authors： Kanda T,Steele R,Ray R,Ray RB

更新日期：2007-11-01 00:00:00

abstract：:We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adul...

journal_title：Journal of virology

authors： Bonavia A,Arbour N,Yong VW,Talbot PJ

更新日期：1997-01-01 00:00:00

abstract：:A new subgroup of avian leukosis virus (ALV) that includes a unique env gene, designated J, was identified recently in England. Sequence analysis of prototype English isolate HPRS-103 revealed several other unique genetic characteristics of this strain and provided information that it arose by recombination between ex...

journal_title：Journal of virology

authors： Benson SJ,Ruis BL,Garbers AL,Fadly AM,Conklin KF

更新日期：1998-12-01 00:00:00