Abstract:
:New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient. Kilogram quantities of impurity have been rapidly separated from grams of purified virus, which have been conveniently concentrated several hundred-fold by the purification process. Experimental vaccines made by these procedures are being evaluated.
journal_name
J Viroljournal_title
Journal of virologyauthors
Reimer CB,Baker RS,Van Frank RM,Newlin TE,Cline GB,Anderson NGdoi
10.1128/JVI.1.6.1207-1216.1967subject
Has Abstractpub_date
1967-12-01 00:00:00pages
1207-16issue
6eissn
0022-538Xissn
1098-5514journal_volume
1pub_type
杂志文章abstract::The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia virus type I (HTLV-I) infection. The HTLV-I regulatory protein Tax and the envelope protein gp46 have been implicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3134-3141.1993
更新日期:1993-06-01 00:00:00
abstract::The goal of an effective AIDS vaccine is to generate immunity that will prevent human immunodeficiency virus 1 (HIV-1) acquisition. Despite limited progress toward this goal, renewed optimism has followed the recent success of the RV144 vaccine trial in Thailand. However, the lack of complete protection in this trial ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00315-12
更新日期:2012-05-01 00:00:00
abstract::Poliovirus infection of HeLa cells results in a rapid inhibition of host protein synthesis by a mechanism that does not affect the translation of poliovirus RNA. It has been suggested that this virus-induced translational control results from inactivation of the cap-binding protein complex, and it has been shown that ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2480-2488.1987
更新日期:1987-08-01 00:00:00
abstract::Translation of aphthovirus RNA is initiated at an internal ribosome entry site (IRES) element, preceding the first functional AUG initiation codon. The effect of mutations at the base of domain 3 of the aphthovirus IRES on translation activity has been analyzed by site-directed mutagenesis and expression of bicistroni...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.992-998.1996
更新日期:1996-02-01 00:00:00
abstract::Merkel cell polyomavirus (MCPyV) is the major cause for Merkel cell carcinoma (MCC), a rare but highly aggressive skin cancer predominantly found in elderly and immunosuppressed patients. The early viral gene products large T-antigen (LT) and small T-antigen (sT) are important for efficient viral DNA replication, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01638-19
更新日期:2020-02-14 00:00:00
abstract::Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-10
更新日期:2010-07-01 00:00:00
abstract::The viral membrane proteins M and E are the minimal requirements for the budding of coronavirus particles. Since the E protein occurs in particles only in trace amounts, the lateral interactions between the M proteins apparently generate the major driving force for envelope formation. By using coimmunoprecipitation an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.4967-4978.2000
更新日期:2000-06-01 00:00:00
abstract::The role of nitric oxide after poliovirus infection of the human HeLa (carcinoma) and U937 (promonocytic) cell lines has been analyzed. Both types of cells produced detectable levels of nitric oxide after poliovirus infection. However, this production was not sufficient to limit viral productivity. On the other hand, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2538-2540.1998
更新日期:1998-03-01 00:00:00
abstract::Levels of the p53 tumor suppressor protein are increased in human cytomegalovirus (HCMV)-infected cells and may be important for HCMV pathogenesis. In normal cells p53 levels are kept low due to an autoregulatory feedback loop where p53 activates the transcription of mdm2 and mdm2 binds and ubiquitinates p53, targetin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3833-3843.2006
更新日期:2006-04-01 00:00:00
abstract::An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1207-1216.1990
更新日期:1990-03-01 00:00:00
abstract::Our previous studies with influenza A viruses indicated that the association of M1 with viral RNA and nucleoprotein (NP) is required for the efficient formation of helical ribonucleoprotein (RNP) and for the nuclear export of RNPs. RNA-binding domains of M1 map to the following two independent regions: a zinc finger m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9585-9591.2004
更新日期:2004-09-01 00:00:00
abstract::Retroviruses and retrotransposons insert into the host genome with no obvious sequence specificity. We examined the target sites of the retroelement ZAM by sequencing each host-ZAM junction in the genome of Drosophila melanogaster. Our overall data provide compelling evidence that ZAM integration machinery recognizes ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.7061-7064.1999
更新日期:1999-08-01 00:00:00
abstract::We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was emp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01349-08
更新日期:2009-02-01 00:00:00
abstract:UNLABELLED:The gastrointestinal mucosa is the primary site where human immunodeficiency virus type 1 (HIV-1) invades, amplifies, and becomes persistently established, and cell-to-cell transmission of HIV-1 plays a pivotal role in mucosal viral dissemination. Mast cells are widely distributed in the gastrointestinal tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03008-15
更新日期:2015-12-30 00:00:00
abstract::Glycosylation of the envelope glycoprotein of vesicular stomatitis virus was examined using virus-infected HeLa cells that were pulse-labeled with radioactive sugar precursors. The intracellular sites of glycosylation and the stepwise elongation of the carbohydrate side chains of the G protein were monitored by membra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.3.646-657.1976
更新日期:1976-12-01 00:00:00
abstract::The susceptibility to tumors induced by raf and raf/myc retroviruses was investigated in BALB/c, C57BL/6, (BALB/c x C57BL/6)F1 and (BALB/c x C57BL/6) backcross mice. Newborn mice were susceptible to neoplasms generated by both viruses, but resistance to raf-induced leukemia developed rapidly in all mice as they mature...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2411-2414.1989
更新日期:1989-05-01 00:00:00
abstract:UNLABELLED:Despite encoding multiple viral proteins that modulate the retinoblastoma (Rb) protein in a manner classically defined as inactivation, human cytomegalovirus (HCMV) requires the presence of the Rb protein to replicate efficiently. In uninfected cells, Rb controls numerous pathways that the virus also command...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01718-15
更新日期:2015-12-01 00:00:00
abstract::The gene encoding the precursor polypeptide of the Marek's disease herpesvirus (MDHV) secretory glycoprotein gp57-65 (formerly identified as A antigen) has been sequenced. Previous results had localized the gene to a 4.6-kilobase (kb) segment of the BamHI B fragment in the unique long region of the MDHV genome. S1 nuc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2373-2379.1988
更新日期:1988-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5'-CAGGTA-3', called ZV, located at nucleotides -17 to -12 relative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.867-877.2001
更新日期:2001-01-01 00:00:00
abstract::BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.30-39.1979
更新日期:1979-10-01 00:00:00
abstract::By superinfection of human immunodeficiency virus type 2 (HIV-2) strain HIV-2ben-infected macaques with simian immunodeficiency virus (SIV) strain SIVmac, we investigated the mutual influences of an apathogenic and a pathogenic virus in vivo. Four rhesus and two cynomolgus monkeys were infected with HIV-2ben in 1988 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1564-1574.1995
更新日期:1995-03-01 00:00:00
abstract::The E6 oncoprotein of high-risk human papillomavirus type 16 (HPV16) interacts with several nuclear transcription factors and coactivators in addition to cytoplasmic proteins, suggesting that nuclear import of HPV16 E6 plays a role in the cellular transformation process. In this study we have investigated the nuclear ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2330-2337.2003
更新日期:2003-02-01 00:00:00
abstract::Alfalfa mosaic virus (AMV) and ilarvirus RNAs are infectious only in the presence of the viral coat protein; therefore, an understanding of coat protein's function is important for defining viral replication mechanisms. Based on in vitro replication experiments, the conformational switch model states that AMV coat pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5752-5761.2005
更新日期:2005-05-01 00:00:00
abstract::Immunity to human immunodeficiency virus virion-like structures or a polyprotein has been examined after DNA immunization with Rev-independent expression vectors. A Gag-Pol fusion protein stimulated cytotoxic T lymphocyte and antibody responses to Gag and Pol, while a Gag-Pol pseudoparticle did not elicit substantial ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4947-4951.2001
更新日期:2001-05-01 00:00:00
abstract::To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1302-1305.1995
更新日期:1995-02-01 00:00:00
abstract::It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.2.785-793.2006
更新日期:2006-01-01 00:00:00
abstract::Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5299-5302.1993
更新日期:1993-09-01 00:00:00
abstract::The Epstein-Barr virus (EBV) is an oncogenic human herpesvirus. EBV latent membrane protein 1 (LMP-1) is a viral oncogene that manifests its oncogenic phenotype through activation of cellular signaling pathways involved in cell growth, survival, differentiation, and transformation. Lytic LMP-1 (lyLMP-1) is a related E...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00180-06
更新日期:2006-08-01 00:00:00
abstract::The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.401-405.1977
更新日期:1977-10-01 00:00:00
abstract::By inhibiting techniques using indirect immunofluorescence tests and indirect immunoelectron microscopy, the G(Gross) soluble antigens (GSA) in the body fluids of AKR and C58 mice, which have a high incidence of spontaneous leukemia, were classified according to the known specificity of G antigens in the murine Gross ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1208-1219.1972
更新日期:1972-12-01 00:00:00