Abstract:
:It was recently shown that the 7a protein of severe acute respiratory syndrome coronavirus induces biochemical changes associated with apoptosis. In this study, the mechanism by which the 7a protein induces apoptosis was examined. The 7a protein was tested for the ability to inhibit cellular gene expression because several proapoptotic viral proteins with this function have previously been identified. 7a protein inhibited expression of luciferase from an mRNA construct that specifically measures translation, whereas inhibitors of transcription and nucleocytoplasmic transport did not. The inhibition of translation and other cellular processes of gene expression have been associated with the induction of a stress response in cells. Western blot analysis using phosphospecific antibodies indicated that 7a protein activated p38 mitogen-activated protein kinase (MAPK), but not c-Jun N-terminal protein kinase/stress-activated protein kinase. Taken together, these data indicate that the induction of apoptosis by the 7a protein may be related to its ability to inhibit cellular translation and activate p38 MAPK.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kopecky-Bromberg SA,Martinez-Sobrido L,Palese Pdoi
10.1128/JVI.80.2.785-793.2006subject
Has Abstractpub_date
2006-01-01 00:00:00pages
785-93issue
2eissn
0022-538Xissn
1098-5514pii
80/2/785journal_volume
80pub_type
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