Abstract:
:We have selected neutralization escape mutants by using a monoclonal antibody (nt-MAb) against a sequential epitope between amino acids 93 through 104 (neutralization antigenic site I) of poliovirus type 1 Mahoney. The majority of mutants were also resistant against five strain-specific nt-MAbs which recognized conformation-dependent epitopes, suggesting that the neutralization antigenic site I must be involved in the formation of such epitopes. An analysis of all mutants by the binding of nt-MAbs and by isoelectric focusing of VP1 allowed discrimination of five classes of mutants. Sequence analysis of mutant RNAs revealed point mutations and deletions in the antibody-binding site.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wiegers K,Uhlig H,Dernick Rdoi
10.1128/JVI.62.5.1845-1848.1988subject
Has Abstractpub_date
1988-05-01 00:00:00pages
1845-8issue
5eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::The internal structural proteins of retroviruses are proteolytically processed from the Gag polyprotein, which alone is able to assemble into virus-like particles when expressed in cells. All Gag proteins contain domains corresponding to the three structural proteins MA, CA, and NC. We have expressed the CA and NC dom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6487-6497.1995
更新日期:1995-10-01 00:00:00
abstract::Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus of mice, is transmitted sexually. We show that this depends on the major virion envelope glycoprotein gp150. gp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-17
更新日期:2017-06-09 00:00:00
abstract::The human cytomegalovirus (HCMV) immediate-early 2 (IE2) transactivator has previously been shown to form intranuclear, dot-like accumulations in association with subnuclear structures known as promyelocytic leukemia protein (PML) nuclear bodies or ND10. We recently observed that IE2 can form dot-like structures even ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01009-07
更新日期:2007-09-01 00:00:00
abstract::Despite being able to use the Bonzo coreceptor as efficiently as CCR5 in transfected cells, pediatric human immunodeficiency virus type 1 isolate P6 was unable to replicate in peripheral blood mononuclear cells (PBMC) lacking the CCR5 receptor. Furthermore, its replication in wild-type PBMC was completely inhibited by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3443-3448.1999
更新日期:1999-04-01 00:00:00
abstract::We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is preserved;...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-15
更新日期:2015-07-01 00:00:00
abstract::Serological surveys have revealed that 30 to 50% of wild-caught African green monkeys have antibodies reactive to simian immunodeficiency virus (SIV), a retrovirus related to human immunodeficiency virus (HIV). Although the nucleotide sequence of one SIVagm isolate, Tyo1, was recently reported, the extent of genetic v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1800-1802.1989
更新日期:1989-04-01 00:00:00
abstract::The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectab...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.6.915-925.1973
更新日期:1973-06-01 00:00:00
abstract::Tankyrase (TNKS) is a telomere-associated poly-ADP ribose polymerase (PARP) that has been implicated along with several telomere repeat binding factors in the regulation of Epstein-Barr virus origin of plasmid replication (OriP). We now show that TNKS1 can bind to the family of repeats (FR) and dyad symmetry regions o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4640-4650.2005
更新日期:2005-04-01 00:00:00
abstract::Pestiviruses prevent alpha/beta interferon (IFN-alpha/beta) production by promoting proteasomal degradation of interferon regulatory factor 3 (IRF3) by means of the viral N(pro) nonstructural protein. N(pro) is also an autoprotease, and its amino-terminal coding sequence is involved in translation initiation. We previ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01509-08
更新日期:2009-01-01 00:00:00
abstract::The infectious entry pathway of duck hepatitis B virus (DHBV) was investigated with primary duck hepatocytes. Virus uptake was measured by a selective PCR technique which allows for the detection of a successful infection without the need for viral replication or gene expression. To test whether DHBV uptake occurs by ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5827-5831.1996
更新日期:1996-09-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) V3 loop is essential for coreceptor binding and principally determines tropism for the CCR5 and CXCR4 coreceptors. Using the dual-tropic virus HIV-1(R3A), we previously made an extensive panel of V3 deletions and identified subdomains within V3 that could differentially ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01751-08
更新日期:2009-04-01 00:00:00
abstract::ICP0 is a transcriptional activating protein required for the efficient replication and reactivation of latent herpes simplex virus 1 (HSV-1). Multiple regions of ICP0 contribute its activity, the most prominent of which appears to be the RING finger, which confers E3 ubiquitin ligase activity. A region in the C termi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02265-10
更新日期:2011-05-01 00:00:00
abstract::Antibody-dependent enhancement of flavivirus infection, which except for dengue virus is without clear proof in vivo, is still under debate. Recently, postexposure immunoglobulin prophylaxis against tick-borne encephalitis virus, a flavivirus, was claimed to possibly have worsened the outcome of infection due to antib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2921-2927.1997
更新日期:1997-04-01 00:00:00
abstract::Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02254-06
更新日期:2007-04-01 00:00:00
abstract::The synthesis of closed circular simian virus 40 (SV40) deoxyribonucleic acid (DNA) containing sequences homologous to host cell DNA depends upon the conditions under which the cells are infected. When BS-C-1 monkey cells were infected with non-plaque-purified virus at low multiplicity of infection [MOI, 0.032 plaque-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.309-316.1972
更新日期:1972-02-01 00:00:00
abstract::Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1). Elevated expression of vascular endothelial growth factor (VEGF) in ATL patients is associated with leukemic cell invasion and infiltration in different organs. The regulatory protein Tax 1 encoded by HTLV-1 plays a pivotal role in T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02166-09
更新日期:2010-05-01 00:00:00
abstract::In contrast to humans, several primate species are believed to have harbored simian immunodeficiency viruses (SIVs) since ancient times. In particular, the geographically dispersed species of African green monkeys (AGMs) are all infected with highly diversified SIVagm viruses at high prevalences (greater than 50% of s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8449-8460.2001
更新日期:2001-09-01 00:00:00
abstract:UNLABELLED:Adeno-associated virus (AAV) has long been known to inhibit helper adenovirus (Ad) replication independently of AAV Rep protein expression. More recently, replication of Ad serotype 5 (Ad5)/AAV serotype 2 (AAV-2) hybrid vectors was shown to be inhibited incisby a sequence near the 3' end of AAVrep, termed th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03120-15
更新日期:2016-03-28 00:00:00
abstract::A restriction map was constructed for the 17.5-kilobase SalI C fragment of SP82 DNA. Unfractionated SP82 DNA, the SalI C fragment, and restriction fragments derived from SalI-C were used as templates for in vitro synthesis by the Bacillus subtilis RNA polymerase, and the resulting transcripts were analyzed by gel elec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.372-382.1981
更新日期:1981-01-01 00:00:00
abstract::We observed two patterns of chemokine expression in the lungs of mice infected with murine gammaherpesvirus 68: peaks of chemokine expression correlated with or occurred after the peak of viral gene expression. Chemokine expression remained elevated through 29 days postinfection. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10518-10523.2002
更新日期:2002-10-01 00:00:00
abstract::The genome-length, dicistronic mRNA of the double-stranded RNA fungal virus Helminthosporium victoriae virus 190S (genus Victorivirus, family Totiviridae) contains two long open reading frames (ORFs) that overlap in the tetranucleotide AUGA. Translation of the downstream ORF, which encodes the RNA-dependent RNA polyme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00364-11
更新日期:2011-07-01 00:00:00
abstract::The hepatitis B virus (HBV) core promoter regulates the transcription of two related RNA products named precore RNA and core RNA. Previous studies indicate that a double-nucleotide mutation that occurs frequently during chronic HBV infection converts a nuclear receptor binding site in the core promoter to the binding ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.5875-5881.2002
更新日期:2002-06-01 00:00:00
abstract::Despite reports of viral genetic defects in persons who control human immunodeficiency virus type 1 (HIV-1) in the absence of antiviral therapy, the extent to which such defects contribute to the long-term containment of viremia is not known. Most previous studies examining for such defects have involved small numbers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00535-08
更新日期:2008-09-01 00:00:00
abstract::Cowpox virus (CPXV) belongs to the genus Orthopoxvirus in the Poxviridae family. It infects a broad range of vertebrates and can cause zoonotic infections. CPXV has the largest genome among the orthopoxviruses and is therefore considered to have the most complete set of genes of all members of the genus. Since CPXV ha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02385-13
更新日期:2014-01-01 00:00:00
abstract::Marek's disease (MD), a highly infectious disease caused by an oncogenic herpesvirus, is one of the few herpesvirus diseases against which live attenuated vaccines are used as the main strategy for control. We have constructed bacterial artificial chromosomes (BACs) of the CVI988 (Rispens) strain of the virus, the mos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.8712-8718.2003
更新日期:2003-08-01 00:00:00
abstract::An adult horse infected with a virulent, cell culture-adapted strain of equine infectious anemia virus (EIAV) developed cyclical thrombocytopenia in which the nadir of platelet counts coincided with peak febrile responses. In order to investigate the mechanism of thrombocytopenia during acute febrile episodes, four ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6242-6251.1991
更新日期:1991-11-01 00:00:00
abstract::In Epstein-Barr virus (EBV)-carrying nonproducer Raji cells, the induction of the viral replicative cycle by chemical treatment is limited to only the early stage and viral DNA synthesis is totally inhibited. We previously showed the absence of two messenger RNAs that are encoded by the BamHI-A fragment of the EBV gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7309-7314.1995
更新日期:1995-11-01 00:00:00
abstract::A novel circular DNA virus sequence is reported from grapevine. The corresponding genomic organization, coding potential, and conserved origin of replication are similar to those of members of the family Geminiviridae, but the genome of 3,206 nucleotides is 4% larger than the largest reported geminiviral genome and sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00943-12
更新日期:2012-07-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV, also called human herpesvirus 8) is linked to the development of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV expresses several proteins that modulate host cell signaling pathways. One of these proteins...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00511-14
更新日期:2014-08-01 00:00:00
abstract::The entire varicella-zoster virus (VZV) genome appears to be present in latently infected human ganglia, but the extent of virus DNA transcription is unknown. Conventional methods to study virus gene transcripts by Northern (RNA) blotting are not feasible, since ganglia are small and VZV DNA is not abundant. To circum...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.7900-7908.1994
更新日期:1994-12-01 00:00:00