Abstract:
:The entire varicella-zoster virus (VZV) genome appears to be present in latently infected human ganglia, but the extent of virus DNA transcription is unknown. Conventional methods to study virus gene transcripts by Northern (RNA) blotting are not feasible, since ganglia are small and VZV DNA is not abundant. To circumvent this problem, we prepared radiolabeled cDNA from ganglionic RNA, hybridized it to Southern blots containing VZV DNA, and demonstrated the presence of a transcript within the SalI C fragment of the virus genome (R. Cohrs, R. Mahalingam, A. N. Dueland, W. Wolf, M. Wellish, and D. H. Gilden, J. Infect. Dis. 166:S24-S29, 1992). To further map VZV transcripts, in the work described here we constructed a cDNA library from poly(A)+ RNA obtained from latently infected human ganglia. Phage DNA isolated from the library was used in PCR amplifications to detect VZV-specific inserts. The specificity of the PCRs was provided by selection of a primer specific for VZV gene 17, 18, 19, 20, or 21 and a second vector-specific primer. VZV gene 21-specific sequences were identified by PCR amplification. The PCR product contained the XhoI cloning site and poly(A)+ sequences between vector and VZV gene 21 sequences. The sequence motif at the 3' end of VZV gene 21, determined by cloning and sequencing of the PCR product, consisted of 49 to 51 nucleotide bases of 3'-untranslated DNA, the termination codon for the VZV gene 21 open reading frame, and DNA sequences reading into the VZV gene 21 open reading frame.
journal_name
J Viroljournal_title
Journal of virologyauthors
Cohrs RJ,Srock K,Barbour MB,Owens G,Mahalingam R,Devlin ME,Wellish M,Gilden DHdoi
10.1128/JVI.68.12.7900-7908.1994subject
Has Abstractpub_date
1994-12-01 00:00:00pages
7900-8issue
12eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by competitive reverse t...
journal_title:Journal of virology
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doi:10.1128/JVI.70.11.7603-7613.1996
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.71.11.8357-8361.1997
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更新日期:1972-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.38.2.430-437.1981
更新日期:1981-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.367-375.1984
更新日期:1984-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.10416-10425.1999
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pub_type: 杂志文章
doi:10.1128/JVI.34.3.615-626.1980
更新日期:1980-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.4.821-833.1974
更新日期:1974-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6540-6550.1999
更新日期:1999-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3235-3240.1998
更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.73.12.9879-9890.1999
更新日期:1999-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2019-08-13 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.12.3.523-533.1973
更新日期:1973-09-01 00:00:00