Abstract:
:The hepatitis B virus (HBV) core promoter regulates the transcription of two related RNA products named precore RNA and core RNA. Previous studies indicate that a double-nucleotide mutation that occurs frequently during chronic HBV infection converts a nuclear receptor binding site in the core promoter to the binding site of the transcription factor hepatocyte nuclear factor-1 (HNF-1) and specifically suppresses the transcription of the precore RNA. This mutation also changes two codons in the overlapping X protein coding sequence. In this report, we demonstrate that the X protein and its mutant X(mt) can physically bind to HNF-1 both in vitro and in vivo. Further analyses indicate that both X and X(mt) can enhance the gene transactivation and the DNA binding activities of HNF-1. This finding demonstrates for the first time that the X protein can stimulate the DNA binding activity of a homeodomain transcription factor. Interestingly, while both X and X(mt) can stimulate the HNF-1 activities, they differ in their effects: a smaller amount of X(mt) is needed to generate greater transactivation and DNA binding activities of HNF-1. This functional difference between X and X(mt) may have important implications in HBV pathogenesis and is apparently why they have different effects on the core promoter bearing the HNF-1 binding site.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li J,Xu Z,Zheng Y,Johnson DL,Ou JHdoi
10.1128/jvi.76.12.5875-5881.2002subject
Has Abstractpub_date
2002-06-01 00:00:00pages
5875-81issue
12eissn
0022-538Xissn
1098-5514journal_volume
76pub_type
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doi:10.1128/JVI.71.9.6757-6764.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00053-06
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6151-6156.1996
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00686-09
更新日期:2009-08-01 00:00:00
abstract::Insertion of a region, including the 18-nucleotide-long intergenic sequence between genes 6 and 7 of mouse hepatitis virus (MHV) genomic RNA, into an MHV defective interfering (DI) RNA leads to transcription of subgenomic DI RNA in helper virus-infected cells (S. Makino, M. Joo, and J. K. Makino, J. Virol. 66:6031-604...
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pub_type: 杂志文章
doi:10.1128/JVI.67.6.3304-3311.1993
更新日期:1993-06-01 00:00:00