Abstract:
:The mechanism by which herpes simplex virus 1 (HSV-1) establishes latency in sensory neurons is largely unknown. Recent studies indicate that epigenetic modifications of the chromatin associated with the latent genome may play a key role in the transcriptional control of lytic genes during latency. In this study, we found both constitutive and facultative types of heterochromatin to be present on the latent HSV-1 genome. Deposition of the facultative marks trimethyl H3K27 and histone variant macroH2A varied at different sites on the genome, whereas the constitutive marker trimethyl H3K9 did not. In addition, we show that in the absence of the latency-associated transcript (LAT), the latent genome shows a dramatic increase in trimethyl H3K27, suggesting that expression of the LAT during latency may act to promote an appropriate heterochromatic state that represses lytic genes but is still poised for reactivation. Due to the presence of the mark trimethyl H3K27, we examined whether Polycomb group proteins, which methylate H3K27, were present on the HSV-1 genome during latency. Our data indicate that Bmi1, a member of the Polycomb repressive complex 1 (PRC1) maintenance complex, associates with specific sites in the genome, with the highest level of enrichment at the LAT enhancer. To our knowledge, these are the first data demonstrating that a virus can repress its gene transcription to enter latency by exploiting the mechanism of Polycomb-mediated repression.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kwiatkowski DL,Thompson HW,Bloom DCdoi
10.1128/JVI.00686-09subject
Has Abstractpub_date
2009-08-01 00:00:00pages
8173-81issue
16eissn
0022-538Xissn
1098-5514pii
JVI.00686-09journal_volume
83pub_type
杂志文章abstract::EBNA2 transcriptional activation and regulated EBNALP coactivation are critical for Epstein-Barr virus-infected primary B-lymphocyte growth transformation. EBNALP coactivation requires the EBNA2 acidic activation domain (E2AD); EBNALP can bind to E2AD. EBNALP has now been found to bind less well to EBNA2 amino acids 1...
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journal_title:Journal of virology
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doi:10.1128/JVI.01253-07
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11097-11107.2004
更新日期:2004-10-01 00:00:00
abstract::The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attac...
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doi:10.1128/JVI.9.4.664-671.1972
更新日期:1972-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8690-8696.1998
更新日期:1998-11-01 00:00:00
abstract::Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic posi...
journal_title:Journal of virology
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journal_title:Journal of virology
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journal_title:Journal of virology
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journal_title:Journal of virology
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更新日期:2017-06-09 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.170-176.1977
更新日期:1977-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
abstract::The E7 proteins of human papillomaviruses (HPVs) promote S-phase reentry in differentiated keratinocytes of the squamous epithelia to support viral DNA amplification. In this study, we showed that nuclear p130 was present in the differentiated strata of several native squamous epithelia susceptible to HPV infection. I...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::The complex evolutionary process of human immunodeficiency virus type 1 (HIV-1) is marked by a high level of genetic variation. It has been shown that the HIV-1 genome is characterized by variable and more constant regions, unequal nucleotide frequencies, and preference for G-to-A substitutions. However, this knowledg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4761-4770.1997
更新日期:1997-06-01 00:00:00
abstract::The lack of a mouse model has hampered an understanding of the pathogenesis and immunity of Marburg hemorrhagic fever (MHF), the disease caused by marburgvirus (MARV), and has created a bottleneck in the development of antiviral therapeutics. Primary isolates of the filoviruses, i.e., ebolavirus (EBOV) and MARV, are n...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
abstract::Previous in vitro analyses have shown that the human immunodeficiency virus type 1 (HIV-1) integrase uses either manganese or magnesium to assemble as a stable complex on the donor substrate and to catalyze strand transfer. We now demonstrate that subsequent to assembly, catalysis of both 3' end processing and strand ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.7005-7011.1997
更新日期:1997-09-01 00:00:00