Measles virus attenuation associated with transcriptional impediment and a few amino acid changes in the polymerase and accessory proteins.

abstract：:Using a recently developed model for in vitro generation of pp65-positive polymorphonuclear leukocytes (PMNLs), we demonstrated that PMNLs from immunocompetent subjects may harbor both infectious human cytomegalovirus (HCMV) and viral products (pp65, p72, DNA, and immediate-early [IE] and pp67 late mRNAs) as early as ...

journal_title：Journal of virology

authors： Gerna G,Percivalle E,Baldanti F,Sozzani S,Lanzarini P,Genini E,Lilleri D,Revello MG

更新日期：2000-06-01 00:00:00

abstract：:Hepatitis E virus genotype 1 strain Sar55 replicated in subcloned Caco-2 intestinal cells and Huh7 hepatoma cells that had been transfected with in vitro transcribed viral genomes, and hepatitis E virions were released into the culture medium of both cell lines. Virus egress from cells depended on open reading frame 3...

journal_title：Journal of virology

authors： Emerson SU,Nguyen HT,Torian U,Burke D,Engle R,Purcell RH

更新日期：2010-09-01 00:00:00

abstract：UNLABELLED:The cytosolic RIG-I (retinoic acid-inducible gene I) receptor plays a pivotal role in the initiation of the immune response against RNA virus infection by recognizing short 5'-triphosphate (5'ppp)-containing viral RNA and activating the host antiviral innate response. In the present study, we generated novel...

journal_title：Journal of virology

authors： Chiang C,Beljanski V,Yin K,Olagnier D,Ben Yebdri F,Steel C,Goulet ML,DeFilippis VR,Streblow DN,Haddad EK,Trautmann L,Ross T,Lin R,Hiscott J

更新日期：2015-08-01 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...

journal_title：Journal of virology

authors： Cannon M,Philpott NJ,Cesarman E

更新日期：2003-01-01 00:00:00

abstract：:Plasmacytoid dendritic cells (pDC) poorly replicate human immunodeficiency virus type 1 (HIV-1) but efficiently transfer HIV-1 to adjacent CD4 T lymphocytes. We found that coculture with T lymphocytes downregulates SAMHD1 expression, enhances HIV-1 replication, and increases pDC maturation and alpha interferon (IFN-α)...

journal_title：Journal of virology

authors： Su B,Lederle A,Laumond G,Ducloy C,Schmidt S,Decoville T,Moog C

更新日期：2014-09-01 00:00:00

abstract：:We have examined the pattern of RNA transcription on the L (late) DNA strand late in the simian virus 40 virus infectious cycle by separating pulse-labeled RNA chains according to size and hybridizing to an ordered series of DNA fragments obtained by restriction enzyme digestion. From this analysis, the 5' end of the ...

journal_title：Journal of virology

authors： Ford JP,Hsu MT

更新日期：1978-12-01 00:00:00

abstract：:Two herpes simplex virus type 1 glycoproteins, gE and gI, have been shown to form a complex that binds the Fc domain of immunoglobulin G (IgG). We demonstrate that this complex is required for the binding of monomeric nonimmune IgG but that gE alone is sufficient for binding polymeric IgG in the form of IgG complexes....

journal_title：Journal of virology

authors： Dubin G,Frank I,Friedman HM

更新日期：1990-06-01 00:00:00

abstract：:We have previously reported that infection with herpes simplex virus type 1 (HSV-1) activates expression of the human immunodeficiency virus type 1 (HIV-1) provirus in T cells. Activation of the HIV-1 provirus correlated with the activation of binding of 55- and 85-kDa proteins to the kappa B enhancer and binding of t...

journal_title：Journal of virology

authors： Vlach J,Pitha PM

更新日期：1993-07-01 00:00:00

abstract：:Hepatitis B virus (HBV) is an enveloped DNA virus that presumably buds at intracellular membranes of infected cells. HBV budding involves two endocytic host proteins, the ubiquitin-interacting adaptor gamma 2-adaptin and the Nedd4 ubiquitin ligase. Here, we demonstrate that HBV release also requires the cellular machi...

journal_title：Journal of virology

authors： Lambert C,Döring T,Prange R

更新日期：2007-09-01 00:00:00

abstract：:Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...

journal_title：Journal of virology

authors： Nuss DL,Koch G

更新日期：1975-01-01 00:00:00

abstract：:We have isolated a new type of temperature-sensitive mutant of simian virus 40 (SV40) that is capable of productive infection in permissive cells but not of maintenance of viral DNA integration in transformed cells at the conditional temperature. Virus development is induced when cells transformed by this mutant are s...

journal_title：Journal of virology

authors： Rothschild H,Lockwood MP

更新日期：1976-08-01 00:00:00

abstract：:Abelson murine leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transformation of immortalized fibroblast lines and pre-B cells. Temperature-sensitive mutations affecting the kinase domain of the protein have demonstrated that the kinase activity is absolutely required for transformation. ...

journal_title：Journal of virology

authors： Mainville CA,Parmar K,Unnikrishnan I,Gong L,Raffel GD,Rosenberg N

更新日期：2001-02-01 00:00:00

abstract：:The temporal regulation of DNA replication is thought to be important for chromosome organization and genome stability. We show here that Epstein-Barr virus (EBV) genomes replicate in mid- to late S phase and that agents that accelerate replication timing of EBV reduce viral genome stability. Hydroxyurea (HU) treatmen...

journal_title：Journal of virology

authors： Zhou J,Snyder AR,Lieberman PM

更新日期：2009-03-01 00:00:00

abstract：:Gene therapy is proposed as a novel therapeutic strategy for treating glioblastoma multiforme (GBM), a devastating brain cancer. In the clinic, antivector immune responses pose formidable challenges. Herein we demonstrate that high-capacity adenovirus vectors (HC-Ads) carrying the conditional cytotoxic gene herpes sim...

journal_title：Journal of virology

authors： King GD,Muhammad AK,Xiong W,Kroeger KM,Puntel M,Larocque D,Palmer D,Ng P,Lowenstein PR,Castro MG

更新日期：2008-05-01 00:00:00

abstract：:Previous studies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of intermediate and late cDNA products. To further characterize this effect, we evaluated the topology of the 5' end of the HIV-1 RNA genome during early...

journal_title：Journal of virology

authors： Galvis AE,Fisher HE,Fan H,Camerini D

更新日期：2017-11-14 00:00:00

abstract：:Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...

journal_title：Journal of virology

authors： Trent DW

更新日期：1977-06-01 00:00:00

abstract：:The fate of polyomavirus genomes in stable transformants was examined in experiments in which rat or hamster cells were infected with mixed viral populations containing either two distinguishable wild types or a wild type plus a transformation-deficient mutant. The results demonstrate that in 90% of the cases in eithe...

journal_title：Journal of virology

authors： Oh SY,Amalfitano A,Friderici K,Chen MC,Fluck MM

更新日期：1990-03-01 00:00:00

abstract：:The early steps of the intracellular trafficking of human papillomavirus type 16 (HPV-16), -31, and -58 pseudovirions were studied by investigating the effects of drugs acting at defined points of endocytosis pathways on virus-like particle-mediated pseudoinfection by overexpression of a dominant-negative form of the ...

journal_title：Journal of virology

authors： Bousarghin L,Touzé A,Sizaret PY,Coursaget P

更新日期：2003-03-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) pathogenesis is dependent on the hematogenous spread of the virus to host tissue. While data suggest that infected monocytes are required for viral dissemination from the blood to the host organs, infected endothelial cells are also thought to contribute to this key step in viral pathogene...

journal_title：Journal of virology

authors： Bentz GL,Jarquin-Pardo M,Chan G,Smith MS,Sinzger C,Yurochko AD

更新日期：2006-12-01 00:00:00

abstract：:All presently available replication-competent proviral clones of human immunodeficiency virus type 1 (HIV-1) are derived from cell culture-amplified virus. Since tissue culture is highly selective for viral strains with an in vitro growth advantage, such clones may not be representative of the biologically relevant vi...

journal_title：Journal of virology

authors： Li Y,Kappes JC,Conway JA,Price RW,Shaw GM,Hahn BH

更新日期：1991-08-01 00:00:00

abstract：:We propose that a nucleotide template-based mechanism facilitates the acquisition of the K65R mutation in subtype C human immunodeficiency virus type 1 (HIV-1). Different patterns of DNA synthesis were observed using DNA templates from viruses of subtype B or C origin. When subtype C reverse transcriptase (RT) was emp...

journal_title：Journal of virology

authors： Coutsinos D,Invernizzi CF,Xu H,Moisi D,Oliveira M,Brenner BG,Wainberg MA

更新日期：2009-02-01 00:00:00

abstract：UNLABELLED:Enterovirus 71 (EV71) recruits various cellular factors to assist in the replication and translation of its genome. Identification of the host factors involved in the EV71 life cycle not only will enable a better understanding of the infection mechanism but also has the potential to be of use in the developm...

journal_title：Journal of virology

authors： Zhang H,Song L,Cong H,Tien P

更新日期：2015-10-01 00:00:00

abstract：:MicroRNAs (miRNAs) are potent RNA regulators of gene expression. Some viruses encode miRNAs, most of unknown function. The majority of viral miRNAs are not conserved, and whether any have conserved functions remains unclear. Here, we report that two human polyomaviruses associated with serious disease in immunocomprom...

journal_title：Journal of virology

authors： Seo GJ,Fink LH,O'Hara B,Atwood WJ,Sullivan CS

更新日期：2008-10-01 00:00:00

abstract：:The synthesis of closed circular simian virus 40 (SV40) deoxyribonucleic acid (DNA) containing sequences homologous to host cell DNA depends upon the conditions under which the cells are infected. When BS-C-1 monkey cells were infected with non-plaque-purified virus at low multiplicity of infection [MOI, 0.032 plaque-...

journal_title：Journal of virology

authors： Lavi S,Winocour E

更新日期：1972-02-01 00:00:00

abstract：:Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary hum...

journal_title：Journal of virology

authors： Spann KM,Tran KC,Chi B,Rabin RL,Collins PL

更新日期：2004-04-01 00:00:00

abstract：:The characteristics of fusion of respiratory syncytial virus (RSV) with HEp-2 cells were studied by the R18 fluorescence dequenching assay of membrane fusion. A gradual increase in fluorescence intensity indicative of virion-cell fusion was observed when R18-labeled RSV was incubated with HEp-2 cells. Approximately 35...

journal_title：Journal of virology

authors： Srinivasakumar N,Ogra PL,Flanagan TD

更新日期：1991-08-01 00:00:00

abstract：:A panel of seven monoclonal antibodies against the relatively conserved CD4-binding domain on human immunodeficiency virus type 1 (HIV-1) gp120 was generated by immunizing mice with purified gp120. These monoclonal antibodies reacted specifically with gp120 in an enzyme-linked immunosorbent assay and Western blots (im...

journal_title：Journal of virology

authors： Sun NC,Ho DD,Sun CR,Liou RS,Gordon W,Fung MS,Li XL,Ting RC,Lee TH,Chang NT

更新日期：1989-09-01 00:00:00

abstract：:Second-site revertants from replication-incompetent molecular clones of human immunodeficiency virus (HIV) contain base substitutions adjacent to the TATA motif. The altered TATA box motifs were analyzed for their effect(s) on virus infectivity, long terminal repeat (LTR)-directed expression in transient transfection ...

journal_title：Journal of virology

authors： Kashanchi F,Shibata R,Ross EK,Brady JN,Martin MA

更新日期：1994-05-01 00:00:00

abstract：:2G12 is a broadly neutralizing anti-HIV-1 monoclonal human IgG1 antibody reactive with a high-mannose glycan cluster on the surface of glycoprotein gp120. A key feature of this very highly mutated antibody is domain exchange of the heavy-chain variable region (V(H)) with the V(H) of the adjacent Fab of the same immuno...

journal_title：Journal of virology

authors： Huber M,Le KM,Doores KJ,Fulton Z,Stanfield RL,Wilson IA,Burton DR

更新日期：2010-10-01 00:00:00

abstract：:We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. ...

journal_title：Journal of virology

authors： Binley JM,Cayanan CS,Wiley C,Schülke N,Olson WC,Burton DR

更新日期：2003-05-01 00:00:00