Generation and characterization of monoclonal antibodies to the putative CD4-binding domain of human immunodeficiency virus type 1 gp120.

abstract：:Borna disease virus (BDV) is a neurotropic virus with a broad host and geographic range. Lewis rats were immunized against BDV with a recombinant vaccinia virus expressing the BDV nucleoprotein and were later infected with BDV to evaluate protection against Borna disease (BD). Relative to animals that were not immuniz...

journal_title：Journal of virology

authors： Lewis AJ,Whitton JL,Hatalski CG,Weissenböck H,Lipkin WI

更新日期：1999-03-01 00:00:00

abstract：:The 72-kDa immediate early 1 (IE1) protein encoded by human cytomegalovirus (hCMV) is a nuclearly localized promiscuous regulator of viral and cellular transcription. IE1 has long been known to associate with host mitotic chromatin, yet the mechanisms underlying this interaction have not been specified. In this study,...

journal_title：Journal of virology

authors： Mücke K,Paulus C,Bernhardt K,Gerrer K,Schön K,Fink A,Sauer EM,Asbach-Nitzsche A,Harwardt T,Kieninger B,Kremer W,Kalbitzer HR,Nevels M

更新日期：2014-01-01 00:00:00

abstract：:DDX21 regulates the biogenesis of rRNA and transcription of ribonucleoprotein genes. Recently, it has been reported that DDX21 regulates the growth of some RNA viruses through various mechanisms, such as inhibiting viral genome replication, suppressing virion assembly and release, and modulating antiviral immune respo...

journal_title：Journal of virology

authors： Hao H,Han T,Xuan B,Sun Y,Tang S,Yue N,Qian Z

更新日期：2019-11-26 00:00:00

abstract：:Natural transmission of human immunodeficiency virus (HIV) occurs at mucosal surfaces. During acute infection, intestinal and other mucosae are preferential sites of virus replication and rapidly become depleted of CD4(+) T cells. Therefore, mucosal immunity may be critical to control both initial infection and the ma...

journal_title：Journal of virology

authors： Zhao X,Zhang M,Li Z,Frankel FR

更新日期：2006-09-01 00:00:00

abstract：:We have previously described several human immunodeficiency virus type 1 (HIV-1) mutants that are characterized by an excessive-RNA-splicing phenotype and reduced virus particle production. In one of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice donor site. This splice sit...

journal_title：Journal of virology

authors： Mandal D,Feng Z,Stoltzfus CM

更新日期：2008-02-01 00:00:00

abstract：:Poliovirus-infected cells undergo an increase in cytoplasmic calcium concentrations from the 4th h postinfection. The protein responsible for this effect was identified by the expression of different poliovirus nonstructural proteins in HeLa cells by using a recombinant vaccinia virus system. Synthesis of protein 2BC ...

journal_title：Journal of virology

authors： Aldabe R,Irurzun A,Carrasco L

更新日期：1997-08-01 00:00:00

abstract：:Cytomegalovirus (CMV) infection is the most common opportunistic infection in immunosuppressed individuals, such as transplant recipients or people living with HIV/AIDS, and congenital CMV is the leading viral cause of developmental disabilities in infants. Due to the highly species-specific nature of CMV, animal mode...

journal_title：Journal of virology

authors： Marsh AK,Willer DO,Ambagala AP,Dzamba M,Chan JK,Pilon R,Fournier J,Sandstrom P,Brudno M,MacDonald KS

更新日期：2011-12-01 00:00:00

abstract：:The HXLF (HindIII-X left reading frame) gene family is a group of five genes that share one or two regions of homology and are arranged in tandem within the short unique component of the human cytomegalovirus genome (K. Weston and B.G. Barrell, J. Mol. Biol. 192:177-208, 1986). These genes were cloned into an SP6 expr...

journal_title：Journal of virology

authors： Gretch DR,Kari B,Gehrz RC,Stinski MF

更新日期：1988-06-01 00:00:00

abstract：:Cells latently infected with herpes simplex virus (HSV) contain nucleosomal DNA similar to that of host cell chromatin. Recent studies have demonstrated that histones in the latency-associated transcript (LAT) promoter and intron regions contain histone modifications permissive for transcription. However, those histon...

journal_title：Journal of virology

authors： Chen Q,Lin L,Smith S,Huang J,Berger SL,Zhou J

更新日期：2007-05-01 00:00:00

abstract：:The generation of cytopathic effects by murine leukemia viruses (MLVs) in different cell types correlates with the ability of the virus to induce thymic lymphoma. We showed that the induction of apoptosis in mink epithelial cells by mink cell focus-forming (MCF) MLV infection results in the accumulation of high levels...

journal_title：Journal of virology

authors： Zhao X,Yoshimura FK

更新日期：2008-03-01 00:00:00

abstract：UNLABELLED:In a genome-wide small interfering RNA (siRNA) screen, we recently identified the interferon (IFN)-inducible protein 35 (IFI35; also known as IFP35) as a factor required for vesicular stomatitis virus (VSV) infection. Studies reported here were conducted to further understand the role and requirement of IFI3...

journal_title：Journal of virology

authors： Das A,Dinh PX,Panda D,Pattnaik AK

更新日期：2014-03-01 00:00:00

abstract：:NF-D is a ubiquitous nuclear factor that has been shown to bind specifically to a DNA element in the polyomavirus regulatory region. In this report, we demonstrate that NF-D is either identical or very similar to a transcription factor that has been variously named YY1, delta, NF-E1, UCRBP, or CF1. Moreover, we show t...

journal_title：Journal of virology

authors： Martelli F,Iacobini C,Caruso M,Felsani A

更新日期：1996-03-01 00:00:00

abstract：:The G glycoprotein of human respiratory syncytial virus (RSV) was identified previously as the viral attachment protein. Although we and others recently showed that G is not essential for replication in vitro, it does affect the efficiency of replication in a cell type-dependent fashion and is required for efficient r...

journal_title：Journal of virology

authors： Teng MN,Collins PL

更新日期：2002-06-01 00:00:00

abstract：:Thirty-six monoclonal antibodies from mice and three from rats were raised against a peptide corresponding to the immunodominant domain of the transmembrane gp41 protein of human immunodeficiency virus (HIV) type 1 (LGLWGCSGKLIC; amino acid residues 598 to 609). Of these, three monoclonal antibodies from the mice and ...

journal_title：Journal of virology

authors： Oldstone MB,Tishon A,Lewicki H,Dyson HJ,Feher VA,Assa-Munt N,Wright PE

更新日期：1991-04-01 00:00:00

abstract：:Influenza viruses unable to express NS1 protein (delNS1) replicate poorly and induce large amounts of interferon (IFN). They are therefore considered candidate viruses for live-attenuated influenza vaccines. Their attenuated replication is generally assumed to result from the inability to counter the antiviral host re...

journal_title：Journal of virology

authors： van Wielink R,Harmsen MM,Martens DE,Peeters BP,Wijffels RH,Moormann RJ

更新日期：2012-11-01 00:00:00

abstract：:Rabies virus (RV) of the Rhabdoviridae family grows in alpha/beta interferon (IFN)-competent cells, suggesting the existence of viral mechanisms preventing IFN gene expression. We here identify the viral phosphoprotein P as the responsible IFN antagonist. The critical involvement of P was first suggested by the observ...

journal_title：Journal of virology

authors： Brzózka K,Finke S,Conzelmann KK

更新日期：2005-06-01 00:00:00

abstract：:Polyomavirus small t antigen was purified from genetically engineered Escherichia coli and used as the immunogen for the production of polyclonal and monoclonal antibodies. A new series of plasmids for increased expression of polyomavirus T antigens or a T antigen-beta-galactosidase fusion protein was constructed by r...

journal_title：Journal of virology

authors： Pallas DC,Schley C,Mahoney M,Harlow E,Schaffhausen BS,Roberts TM

更新日期：1986-12-01 00:00:00

abstract：:The purpose of this study was to define the relationship between herpes simplex virus (HSV) latency and in vivo ganglionic reactivation. Groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type HSV type 1 strain 17syn+. Reactivation of the ...

journal_title：Journal of virology

authors： Sawtell NM

更新日期：1998-08-01 00:00:00

abstract：:Infection by rat virus has been studied in cultures of rat embryo cells to evaluate the Margolis-Kilham hypothesis that the virus preferentially infects tissues with actively dividing cells. An enhancement of infection was seen in cultures infected 10 hr after fresh medium was added as compared to infection of station...

journal_title：Journal of virology

authors： Tennant RW,Layman KR,Hand RE

更新日期：1969-12-01 00:00:00

abstract：:Rigorous investigation of many functions encoded by cytomegaloviruses (CMVs) requires analysis in the context of virus-host interactions. To facilitate the construction of rhesus CMV (RhCMV) mutants for in vivo studies, a bacterial artificial chromosome (BAC) containing an enhanced green fluorescent protein (EGFP) cas...

journal_title：Journal of virology

authors： Chang WL,Barry PA

更新日期：2003-05-01 00:00:00

abstract：:The herpesvirus saimiri genome encodes a complement control protein homolog (CCPH). Stable mammalian cell transfectants expressing a recombinant transmembrane form of CCPH (mCCPH) or a 5'FLAG epitope-tagged mCCPH (5'FLAGmCCPH) conferred resistance to complement-mediated cell damage by inhibiting the lytic activity of ...

journal_title：Journal of virology

authors： Fodor WL,Rollins SA,Bianco-Caron S,Rother RP,Guilmette ER,Burton WV,Albrecht JC,Fleckenstein B,Squinto SP

更新日期：1995-06-01 00:00:00

abstract：:mRNA's homologous to the herpes simplex virus type 1 DNA restriction endonuclease fragment BamHI p, which contains the thymidine kinase gene, have been identified and mapped by hybrid-arrested translation and mRNA selection. Such mRNA's, when translated in vitro, directed the synthesis of polypeptides of apparent mole...

journal_title：Journal of virology

authors： Preston CM,McGeoch DJ

更新日期：1981-05-01 00:00:00

abstract：:Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t...

journal_title：Journal of virology

authors： Haffar OK,Nakamura GR,Berman PW

更新日期：1990-06-01 00:00:00

abstract：:The T-helper cell response to the internal proteins of rabies virus was investigated. The rabies virus nucleoprotein was shown to be a major target antigen for T-helper cells that cross-react between rabies and rabies-related viruses. T-helper cells were assayed in vitro by testing virus-induced lymphocytes for lympho...

journal_title：Journal of virology

authors： Ertl HC,Dietzschold B,Gore M,Otvos L Jr,Larson JK,Wunner WH,Koprowski H

更新日期：1989-07-01 00:00:00

abstract：:Poliovirus mutants in neutralizing antigenic site 3B were constructed by replacing the glutamic acid residue at amino acid 74 of capsid protein VP2 (VP2074E), using site-specific mutagenesis methods. All viable mutants display small-plaque phenotypes. Characterization of these mutants indicates that capsid assembly is...

journal_title：Journal of virology

authors： Reynolds C,Birnby D,Chow M

更新日期：1992-03-01 00:00:00

abstract：:NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In t...

journal_title：Journal of virology

authors： Koch JO,Bartenschlager R

更新日期：1999-09-01 00:00:00

abstract：:Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis B e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 to 1926 including the core promoter (nt 1742 to 1849) and the pre-C region (nt 1814 to 1900). Mutations in the core promoter or those in the pre-C region, or bot...

journal_title：Journal of virology

authors： Okamoto H,Tsuda F,Akahane Y,Sugai Y,Yoshiba M,Moriyama K,Tanaka T,Miyakawa Y,Mayumi M

更新日期：1994-12-01 00:00:00

abstract：:Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce...

journal_title：Journal of virology

authors： Cladel NM,Budgeon LR,Cooper TK,Balogh KK,Hu J,Christensen ND

更新日期：2013-08-01 00:00:00

abstract：:Ebola virus (EBOV) causes severe hemorrhagic fever, for which therapeutic options are not available. Preventing the entry of EBOV into host cells is an attractive antiviral strategy, which has been validated for HIV by the FDA approval of the anti-HIV drug enfuvirtide. To identify inhibitors of EBOV entry, the EBOV en...

journal_title：Journal of virology

authors： Basu A,Li B,Mills DM,Panchal RG,Cardinale SC,Butler MM,Peet NP,Majgier-Baranowska H,Williams JD,Patel I,Moir DT,Bavari S,Ray R,Farzan MR,Rong L,Bowlin TL

更新日期：2011-04-01 00:00:00

abstract：:The human cytidine deaminase Apobec3F (h-A3F), a protein related to the previously recognized antiviral factor Apobec3G (h-A3G), has antiviral activity against human immunodeficiency virus type 1 (HIV-1) that is suppressed by the viral protein Vif. The mechanism of HIV-1 Vif-mediated suppression of h-A3F is not fully ...

journal_title：Journal of virology

authors： Liu B,Sarkis PT,Luo K,Yu Y,Yu XF

更新日期：2005-08-01 00:00:00