Abstract:
:Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary human peripheral blood monocytes. Results with NS1 and NS2 single- and double-gene-deletion viruses indicated that the two proteins function independently as well as coordinately to achieve the full inhibitory effect, with NS1 having a greater independent role. The relative contributions of the individual NS proteins were the converse of that recently described for bovine RSV (J. F. Valarcher, J. Furze, S. Wyld, R. Cook, K. K. Conzelmann, and G. Taylor, J. Virol. 77:8426-8439, 2003). This pattern of inhibition by HRSV NS1 and NS2 also extended to the newly described antiviral cytokines IFN-lambda 1, -2 and -3.
journal_name
J Viroljournal_title
Journal of virologyauthors
Spann KM,Tran KC,Chi B,Rabin RL,Collins PLdoi
10.1128/jvi.78.8.4363-4369.2004subject
Has Abstractpub_date
2004-04-01 00:00:00pages
4363-9issue
8eissn
0022-538Xissn
1098-5514journal_volume
78pub_type
杂志文章abstract::Mutations introduced into the capsid gene of duck hepatitis B virus (DHBV) were tested for their effects on viral DNA synthesis and assembly of enveloped viruses. Four classes of mutant phenotypes were observed among a series of deletions of covering the 3' end of the capsid open reading frame. Class I mutant capsids ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2511-2517.1991
更新日期:1991-05-01 00:00:00
abstract::Studies of rotavirus morphogenesis, transport, and release have shown that although these viruses are released from the apical surface of polarized intestinal cells before cellular lysis, they do not follow the classic exocytic pathway. Furthermore, increasing evidence suggests that lipid rafts actively participate in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3935-3946.2006
更新日期:2006-04-01 00:00:00
abstract::The hepatitis C virus (HCV) encodes a large polyprotein; therefore, all viral proteins are produced in equimolar amounts regardless of their function. The aim of our study was to determine the ratio of nonstructural proteins to RNA that is required for HCV RNA replication. We analyzed Huh-7 cells harboring full-length...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13594-13605.2005
更新日期:2005-11-01 00:00:00
abstract::The matrix domain of human immunodeficiency virus type 1 Gag polyprotein was studied for its role in virus assembly. Deletion and substitution mutations caused a dramatic reduction in virus production. Mutant Gag polyproteins were myristoylated and had a high affinity for membrane association. Immunofluorescence stain...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6387-6394.1993
更新日期:1993-11-01 00:00:00
abstract::Adeno-associated virus (AAV) vectors are being considered for in vivo applications of gene therapy in the treatment of a variety of disorders. This study evaluates the biology of second-generation vectors based on the novel serotypes AAV7 and AAV8 and containing self-complementary genomes in the nonhuman primate liver...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00526-06
更新日期:2006-06-01 00:00:00
abstract::The herpes simplex virus (HSV) capsid is released into the cytoplasm after fusion of viral and host membranes, whereupon dynein-dependent trafficking along microtubules targets it to the nuclear envelope. Binding of the capsid to the nuclear pore complex (NPC) is mediated by the capsid protein pUL25 and the capsid-tet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00641-17
更新日期:2017-07-12 00:00:00
abstract::Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is wid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.5.2998-3008.2005
更新日期:2005-03-01 00:00:00
abstract::Hepatitis A virus (HAV) 3C proteinase expressed in Escherichia coli was purified to homogeneity, and its cleavage specificity towards various parts of the viral polyprotein was analyzed. Intermolecular cleavage of the P2-P3 domain of the HAV polyprotein gave rise to proteins 2A, 2B, 2C, 3ABC, and 3D, suggesting that i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1727-1733.1995
更新日期:1995-03-01 00:00:00
abstract::The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3740-3748.1992
更新日期:1992-06-01 00:00:00
abstract::Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the procapsid. Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently. To determine whether the association of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2118-2126.2006
更新日期:2006-03-01 00:00:00
abstract::Cells of the established MvlLu mink line spontaneously released a reverse transcriptase-containing virus after long-term passage in tissue culture. By molecular hybridization, DNA of normal mink cells was found to possess extensive nucleotide sequence homology with a reverse-transcription product of the viral genome, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.129-137.1978
更新日期:1978-01-01 00:00:00
abstract::Poliovirus has been studied as a live recombinant vaccine vector because of its attractive characteristics. The genetic instability, however, has hampered recombinant polioviruses (PVs) from being developed as an appropriate vaccine. A variety of different foreign inserts were cloned directly into our poliovirus Sabin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1649-1662.2002
更新日期:2002-02-01 00:00:00
abstract::The oncolytic herpes simplex virus (HSV) that has been approved for clinical practice and those HSVs in clinical trials are attenuated viruses, often with the neurovirulence gene γ134.5 and additional genes deleted. One strategy to engineer nonattenuated oncolytic HSVs consists of retargeting the viral tropism to a ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00067-17
更新日期:2017-04-28 00:00:00
abstract::In order to study primate lentivirus evolution in the Colobinae subfamily, in which only one simian immunodeficiency virus (SIV) has been described to date, we screened additional species from the three different genera of African colobus monkeys for SIV infection. Blood was obtained from 13 West African colobids, and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.744-748.2003
更新日期:2003-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV), a positive-strand RNA virus that belongs to the Arteriviridae family of Nidovirales, has been identified as the causative agent of PRRS. Nsp1alpha is the amino (N)-terminal protein in a polyprotein encoded by the PRRSV genome and is reported to be cru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02579-08
更新日期:2009-11-01 00:00:00
abstract::Autophagy is an intracellular degradation pathway that provides a host defense mechanism against intracellular pathogens. However, many viruses exploit this mechanism to promote their replication. This study shows that lytic induction of Epstein-Barr virus (EBV) increases the membrane-bound form of LC3 (LC3-II) and LC...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02033-14
更新日期:2014-10-01 00:00:00
abstract:UNLABELLED:Cell culture systems reproducing virus replication can serve as unique models for the discovery of novel bioactive molecules. Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a fungus-derived compound, as an inhibitor of the liver X receptor (LXR). NeoB was init...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00856-16
更新日期:2016-09-29 00:00:00
abstract::The EICP0 protein of equine herpesvirus 1 (EHV-1) is an early, viral regulatory protein that independently trans-activates EHV-1 immediate-early (IE), early, gamma1 late, and gamma2 late promoters. To assess whether this powerful trans-activator functions in conjunction with three other EHV-1 regulatory proteins to ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.3.1200-1208.2000
更新日期:2000-02-01 00:00:00
abstract::The susceptibility to tumors induced by raf and raf/myc retroviruses was investigated in BALB/c, C57BL/6, (BALB/c x C57BL/6)F1 and (BALB/c x C57BL/6) backcross mice. Newborn mice were susceptible to neoplasms generated by both viruses, but resistance to raf-induced leukemia developed rapidly in all mice as they mature...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2411-2414.1989
更新日期:1989-05-01 00:00:00
abstract::From purified linear and superhelical DNAs, the restriction endonuclease maps of four xenotropic murine leukemia virus DNAs from NFS, NZB, BALB/c, and AKR mice were determined with ten restriction endonucleases. Each xenotropic proviral DNA was found to be a unique restriction endonuclease map, with differences in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.963-970.1981
更新日期:1981-12-01 00:00:00
abstract::Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3904-3908.1992
更新日期:1992-06-01 00:00:00
abstract::Characterization of endogenous ecotropic Akv proviruses in DNA of low and high leukemic mouse strains revealed the presence of one to six copies of the Akv genome per haploid genome equivalent integrated in the germ line. Low leukemic strains analyzed so far contained only one complete copy of the Akv proviral DNA. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.3.901-908.1982
更新日期:1982-03-01 00:00:00
abstract::The full-length viral RNA of human immunodeficiency virus type 1 (HIV-1) functions both as the mRNA for the viral structural proteins Gag and Gag/Pol and as the genomic RNA packaged within viral particles. The packaging signal which Gag recognizes to initiate genome encapsidation is in the 5' untranslated region (UTR)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02596-05
更新日期:2006-11-01 00:00:00
abstract::African swine fever virus (ASFV) is a complex, cytoplasmic double-stranded DNA (dsDNA) virus that is currently expanding throughout the world. Currently, circulating virulent genotype II Armenia/07-like viruses cause fatal disease in pigs and wild boar, whereas attenuated strains induce infections with various levels ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02298-18
更新日期:2019-05-29 00:00:00
abstract::The small RNA segment (S segment) of Uukuniemi (UUK) virus encodes two proteins, the nucleocapsid protein (N) and a nonstructural protein (NSs), by an ambisense strategy. The function of NSs has not been elucidated for any of the bunyaviruses expressing this protein. We have now expressed the N and NSs proteins in Sf9...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4233-4241.1992
更新日期:1992-07-01 00:00:00
abstract::All herpesviruses have a layer of protein called the tegument that lies between the virion membrane and the capsid. The tegument consists of multiple, virus-encoded protein species that together can account for nearly half the total virus protein. To clarify the structure of the tegument and its attachment to the caps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-09
更新日期:2009-08-01 00:00:00
abstract::Parvovirus capsids are assembled from multiple forms of a single protein and are quite stable structurally. However, in order to infect cells, conformational plasticity of the capsid is required and this likely involves the exposure of structures that are buried within the structural models. The presence of functional...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00972-08
更新日期:2008-11-01 00:00:00
abstract::Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orienta...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.494-499.1986
更新日期:1986-08-01 00:00:00
abstract::The localization of H-1 viral replicative-form double-stranded DNA and progeny single-stranded DNA replication in parasynchronously infected, simian virus 40-transformed newborn human kidney cells was studied with high-resolution electron microscope autoradiography (80-nm silver grains). We analyzed wild-type H-1 and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.349-360.1978
更新日期:1978-01-01 00:00:00
abstract::The nuclear egress complex (NEC) is required for efficient translocation of newly synthesized herpesvirus nucleocapsids from the nucleus to the cytosol. It consists of the type II membrane protein pUL34 which interacts with pUL31 at the inner nuclear membrane (INM). To map regions within pUL34 required for nuclear mem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00021-13
更新日期:2013-04-01 00:00:00