Abstract:
:The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both of the 5'-TGACTAA-3' cis elements capable of interacting with the AP1 transcription factor, which is composed either of homodimers or heterodimers of the Jun products or of heterodimers of Jun and Fos. Mutation of both elements completely abolished P105 promoter activity in human keratinocytes. We show that either AP1 site can interact efficiently in vitro with any of the three different Jun products as heterodimers with c-Fos. However, in nuclear extracts prepared from human keratinocytes, JunB was the predominant Jun component bound to the DNA probe containing this cis element. These results implicate JunB as an important factor in human papillomavirus type 18 transcription in keratinocytes and strongly suggest a potential role of this Jun gene product in the tissue-specific transcription of the genital papillomaviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Thierry F,Spyrou G,Yaniv M,Howley Pdoi
10.1128/JVI.66.6.3740-3748.1992subject
Has Abstractpub_date
1992-06-01 00:00:00pages
3740-8issue
6eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::Epstein-Barr virus (EBV) is a herpesvirus that infects cells by fusing its lipid envelope with the target cell membrane. The fusion process requires the actions of viral glycoproteins gH, gL, and gB for entry into epithelial cells and additionally requires gp42 for entry into B cells. To further study the roles of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00572-06
更新日期:2006-10-01 00:00:00
abstract::Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with the major viral proteins, p40 and p23. Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments and synthetic peptides were analyzed to characterize the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8133-8137.1996
更新日期:1996-11-01 00:00:00
abstract::Experiments with St. Louis encephalitis (SLE) virus have shown that neutral red enhances the plaque size in duck embryo cell cultures. This may represent a new method for genetic studies of SLE virus population. In a mosquito pool specimen NR(+) and NR(-) particles were demonstrated. By intracerebral passage in mice, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.3.178-181.1968
更新日期:1968-03-01 00:00:00
abstract::The importance of neutralizing antibodies (NAbs) in protection against hepatitis C virus (HCV) remains controversial. We infused a chimpanzee with H06 immunoglobulin from a genotype 1a HCV-infected patient and challenged with genotype strains efficiently neutralized by H06 in vitro. Genotype 1a NAbs afforded no protec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01194-15
更新日期:2015-09-01 00:00:00
abstract::Measles virus (MV) can infect the central nervous system and, in rare cases, causes subacute sclerosing panencephalitis, characterized by a progressive degeneration of neurons. The route of MV transmission in neurons was investigated in cultured rat hippocampal slices by using MV expressing green fluorescent protein. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5720-5728.2002
更新日期:2002-06-01 00:00:00
abstract::Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.207-217.1997
更新日期:1997-01-01 00:00:00
abstract::CH/S is a virulent porcine epidemic diarrhea virus (PEDV) strain and is used as the virulent strain to evaluate the protection rates of vaccines against PEDV infection in China. Here, we report the complete genome sequence of strain CH/S, which may aid in understanding the molecular characteristics of this strain. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06024-11
更新日期:2011-11-01 00:00:00
abstract::The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-05
更新日期:2006-07-01 00:00:00
abstract::The ICP18.5 gene (UL28) of herpes simplex virus type 1 is a member of a well-conserved gene family among herpesviruses and is thought to play a role in localization of viral glycoproteins. We have cloned, sequenced, and expressed the entire pseudorabies virus (PRV) ICP18.5 open reading frame in Escherichia coli as a C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3746-3758.1991
更新日期:1991-07-01 00:00:00
abstract::Polyacrylamide gel electrophoresis and tryptic peptide fingerprint analysis of the proteins made in a cell-free system derived from L-cells and immunoprecipitated with simian virus 40 (SV40) anti-T serum demonstrated that both SV40 large-T and small-T antigens are synthesized in vitro in response to mRNA isolated from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.154-170.1978
更新日期:1978-10-01 00:00:00
abstract::Dengue virus (DENV) is a mosquito-borne virus that infects upward of 300 million people annually and has the potential to cause fatal hemorrhagic fever and shock. While the parameters contributing to dengue immunopathogenesis remain unclear, the collapse of redox homeostasis and the damage induced by oxidative stress ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-20
更新日期:2020-11-23 00:00:00
abstract::The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.553-560.1999
更新日期:1999-01-01 00:00:00
abstract::The nucleotide sequence of the region of human polyoma virus JC DNA between 0.5 and 0.7 map units from a unique EcoRI cleavage site was determined and compared with those of the corresponding regions of another human polyoma virus, BK, and simian virus 40 DNAs. Within this region consisting of 945 base pairs, we locat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.73-79.1983
更新日期:1983-01-01 00:00:00
abstract::Sendai virus (SV) and human parainfluenza virus type 1 (hPIV1) have genomes consisting of nonsegmented negative-sense RNA in which the six genes are separated by well-conserved intergenic (IG) sequences and transcriptional start (S) and end signals. In hPIV1-infected cells, transcriptional termination at the M-F gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8244-8251.2002
更新日期:2002-08-01 00:00:00
abstract::Ebola virus (EBOV) continues to pose a significant threat to human health, as evidenced by the 2013-2016 epidemic in West Africa and the ongoing outbreak in the Democratic Republic of the Congo. EBOV causes hemorrhagic fever, organ damage, and shock culminating in death, with case fatality rates as high as 90%. This h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00250-20
更新日期:2020-05-18 00:00:00
abstract::The degree of genetic heterogeneity of DNA virus populations in nature and its consequences for disease control are virtually unknown. The parvovirus minute virus of mice (MVMi) was used here to investigate (i) the frequency of antibody-escape mutants in populations of a DNA virus and (ii) the ability of a DNA virus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2701-2708.2003
更新日期:2003-02-01 00:00:00
abstract::Five hepatoma cell lines, including CZHC/8571, PLC/PRF/5, Hep3B, HepG2, and HUH7, were inoculated with three diverse isolates of human immunodeficiency virus type 1 (HIV-1). Productive infection was noted in all hepatoma cell lines, and expression of viral p24 antigen lasted for over 3 months, but its level decreased ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2553-2559.1990
更新日期:1990-06-01 00:00:00
abstract::An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.5017-5020.2003
更新日期:2003-04-01 00:00:00
abstract::Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3904-3908.1992
更新日期:1992-06-01 00:00:00
abstract::The hemagglutinin-neuraminidase (HN) protein of paramyxoviruses carries out three discrete activities that each affect the ability of HN to promote viral fusion and entry: receptor binding, receptor cleaving (neuraminidase), and triggering of the fusion protein. The interrelationship between the receptor binding and f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02617-06
更新日期:2007-04-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is divided into three subgroups, A, B, and C, based on sequence divergence at the left end of genomic DNA in which the saimiri transforming protein (STP) resides. Subgroup A and C strains transform primary common marmoset lymphocytes to interleukin-2-independent growth, whereas subgroup B str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.8102-8110.2000
更新日期:2000-09-01 00:00:00
abstract::Canine parvovirus type 2 (CPV-2) is a severe enteric pathogen of dogs, causing high mortality in unvaccinated dogs. After emerging, CPV-2 spread rapidly worldwide. However, there is now some evidence to suggest that international transmission appears to be more restricted. In order to investigate the transmission and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01576-10
更新日期:2011-08-01 00:00:00
abstract::Whether a genetic informational nucleic acid is required for the infectivity of transmissible spongiform encephalopathies is central to the debate about the infectious agent. Here we report that an infectious prion formed with bacterially expressed recombinant prion protein plus synthetic polyriboadenylic acid and syn...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06216-11
更新日期:2012-02-01 00:00:00
abstract::By marker rescue with cloned herpes simplex virus 2 DNA fragments, we have mapped the temperature-sensitive mutations of a series of herpes simplex virus 2 mutants to a region of the herpes simplex virus 2 genome that lies within or near the coding sequences for the major DNA-binding protein, ICP8. In cells infected w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.1.332-342.1983
更新日期:1983-01-01 00:00:00
abstract::African green monkeys can maintain long-term persistent infection with simian immunodeficiency viruses (SIVagm) without developing AIDS and thus provide an important model for understanding mechanisms of natural host resistance to disease. This study assessed the levels and anatomic distribution of SIVagm in healthy, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2262-2275.2001
更新日期:2001-03-01 00:00:00
abstract::We have identified a trans-dominant mutant form of the herpes simplex virus (HSV) DNA-binding protein ICP8 which inhibits viral replication. When expressed by the V2.6 cell line, the mutant gene product inhibited wild-type HSV production by 50- to 150-fold when the multiplicity of infection was less than 5. Production...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2666-2675.1991
更新日期:1991-05-01 00:00:00
abstract::Replication and assembly of hepatitis C virus (HCV) depend on the host's secretory and lipid-biosynthetic machinery. Viral replication occurs on endoplasmic reticulum (ER)-derived modified membranes, while viral assembly is thought to occur on lipid droplets (LDs). A physical association and coordination between the v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00496-12
更新日期:2012-06-01 00:00:00
abstract::The sequence of 2,191 nucleotides encoding the env gene of murine retrovirus Akv was determined by using a molecular clone of the Akv provirus. Deduction of the encoded amino acid sequence showed that a single open reading frame encodes a 638-amino acid precursor to gp70 and p15E. In addition, there is a typical leade...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.519-529.1982
更新日期:1982-05-01 00:00:00
abstract::Lipid rafts are proposed to function as platforms for both receptor signaling and trafficking. Following interaction with antigenic peptides, the T-cell receptor (TCR) rapidly translocates to lipid rafts, where it transmits signals and subsequently undergoes endocytosis. The Tip protein of herpesvirus saimiri (HVS), w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.9041-9051.2003
更新日期:2003-08-01 00:00:00
abstract::Two panels of monoclonal antibodies (MAbs) specific for the influenza A virus PA and PB2 polypeptides have been obtained from mice immunized with denatured proteins produced in Escherichia coli. All MAbs (13 specific for the PA polypeptide and 8 specific for the PB2 protein) reacted to the corresponding influenza viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.6900-6909.1994
更新日期:1994-11-01 00:00:00