Abstract:
:The localization of H-1 viral replicative-form double-stranded DNA and progeny single-stranded DNA replication in parasynchronously infected, simian virus 40-transformed newborn human kidney cells was studied with high-resolution electron microscope autoradiography (80-nm silver grains). We analyzed wild-type H-1 and ts1 H-1 (a conditional mutant defective in progeny single-stranded DNA synthesis). The proportion of the total DNA synthesis that was viral was estimated to be >90% by comparing the amount of [(3)H]thymidine uptake in cultures infected with wild-type H-1 versus ts14 (an H-1 mutant defective in DNA replication). Simultaneous staining with cytochrome c-conjugated anti-H-1 immunoglobulin G was performed to ensure that cells incorporating [(3)H]thymidine (2- to 60-min pulses) were H-1 infected. The sites of H-1 replicative-form (in ts1-infected cells) and progeny (in wild-type-infected cells) DNA synthesis were identical. Immunospecifically labeled nuclei at the earliest stages of infection exhibited dense clusters of silver grains over material extruded from nucleolar fibrillar centers. These foci became larger with increasing cellular damage, forming a limited number of H-1 DNA synthetic centers in the euchromatin. Each island-like focus was surrounded by tufts of heterochromatin containing high concentrations of unassembled H-1 capsid proteins. In late phases of infection, the heterochromatin became completely marginated, and the nucleoplasm contained only euchromatin that exhibited randomly distributed sites of H-1 DNA replication. This indicates that H-1 DNA synthesis begins at localized euchromatic or nucleolar sites and then spreads outward. Immunostained heterochromatin and nucleolar chromatin never incorporated [(3)H]thymidine. Our results suggest that H-1 proteins and cellular cofactors associated with the fibrillar component of the nucleolus and the euchromatin may play a role in the regulation of H-1 DNA synthesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Singer II,Rhode SL 3rddoi
10.1128/JVI.25.1.349-360.1978subject
Has Abstractpub_date
1978-01-01 00:00:00pages
349-60issue
1eissn
0022-538Xissn
1098-5514journal_volume
25pub_type
杂志文章abstract::The Epstein-Barr virus BZLF1 gene product ZEBRA is a DNA-binding protein that is partially homologous to c-Fos, binds specifically to AP-1 sites, and can induce the lytic cycle in latently infected B lymphocytes. Induction of the viral lytic cycle can also be achieved by treatment with the phorbol ester 12-O-tetrade-c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4549-4552.1990
更新日期:1990-09-01 00:00:00
abstract::Nine virulent bacteriophages of the anaerobe Corynebacterium (Propionibacterium) acnes, the P-a series, are DNA phages, with long, curved nonretractile tails (130 nm) without tail plates or fibers. They have isometric heads (420 by 460 nm), and are placed in Bradley's group B-1. There is permanent plaque suppression a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1268-1273.1974
更新日期:1974-11-01 00:00:00
abstract::The influenza A virus (IAV) envelope protein hemagglutinin binds α2,6- or α2,3-linked sialic acid as a host cell receptor. Bat IAV subtypes H17N10 and H18N11 form an exception to this rule and do not bind sialic acid but enter cells via major histocompatibility complex (MHC) class II. Here, we review current knowledge...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.01357-19
更新日期:2020-01-31 00:00:00
abstract::Among several proposed cellular receptors for bovine viral diarrhea virus (BVDV), the low-density lipoprotein (LDL) receptor is of special interest because it is also considered a receptor for the related hepatitis C virus. It has been reported that an anti-LDL receptor monoclonal antibody blocked the infection of bov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01589-06
更新日期:2006-11-01 00:00:00
abstract:UNLABELLED:Next-generation sequence analysis of virus-like particles (VLPs) produced during agroinfiltration of cucumber necrosis virus (CNV) coat protein (CP) and of authentic CNV virions was conducted to assess if host RNAs can be encapsidated by CNV CP. VLPs containing host RNAs were found to be produced during agro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-15
更新日期:2015-11-01 00:00:00
abstract::Retroelements are important evolutionary forces but can be deleterious if left uncontrolled. Members of the human APOBEC3 family of cytidine deaminases can inhibit a wide range of endogenous, as well as exogenous, retroelements. These enzymes are structurally organized in one or two domains comprising a zinc-coordinat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01491-09
更新日期:2009-12-01 00:00:00
abstract::Temperature-sensitive (ts) mutants were isolated from the baculovirus Autographa californica (alfalfa looper) MNPV, grown in Spodoptera frugiperda (fall armyworm) cells in the presence of N-methyl-N'-nitro-N-nitrosoguanidine. Of 567 plaque isolates screened, 27 were temperature sensitive (ts), representing a mutation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.190-198.1979
更新日期:1979-07-01 00:00:00
abstract::Type I interferon is induced shortly following viral infection and represents a first line of host defense against a majority of viral pathogens. Not surprisingly, both replication and latency of gammaherpesviruses, ubiquitous cancer-associated pathogens, are attenuated by type I interferon, although the mechanism of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03278-13
更新日期:2014-02-01 00:00:00
abstract::Infection of humans with wild-type measles virus leads to strong immune suppression and secondary infections, whereas immunization with an attenuated vaccine strain does not. Using the cotton rat model (Sigmodon hispidus), we investigated whether vaccine and wild-type viruses differ in viral spread and whether this is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.150-158.2003
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:Marek's disease virus (MDV), an alphaherpesvirus, is the causative agent of a lethal disease in chickens characterized by generalized nerve inflammation and rapid lymphoma development. The extensive colinearity of the MDV genome with those of related herpesviruses has eased functional characterization of man...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02687-14
更新日期:2015-01-15 00:00:00
abstract::A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the four HgaI cleavage sites were mapped at 0.02, 0.14, 0.27, and 0.48 fractional lengths, clockwise, from the single EcoR...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.793-798.1976
更新日期:1976-05-01 00:00:00
abstract::Human herpesvirus 8 (HHV8) downregulates major histocompatibility complex (MHC) class I complexes from the plasma membrane via two of its genes, K3 and K5. The N termini of K3 and K5 contain a plant homeodomain (PHD) predicted to be structurally similar to RING domains found in E3 ubiquitin ligases. In view of the imp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5522-5531.2002
更新日期:2002-06-01 00:00:00
abstract::Human La protein has been implicated in facilitating internal ribosome entry site (IRES)-mediated translation of hepatitis C virus (HCV). Earlier, we demonstrated that the RNA recognition motif (RRM) encompassing residues 112 to 184 of La protein [La (112-184)] interacts with the HCV IRES near the initiator AUG codon....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00924-08
更新日期:2008-12-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) tegument is the least understood component of the virion, and the mechanism of tegument assembly and incorporation into virions during viral egress has not yet been elucidated. In the present study, the addition of tegument proteins (VP13/14, VP16, VP22, and US9) and envelope gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9934-9951.2002
更新日期:2002-10-01 00:00:00
abstract::A soluble system was developed that could support DNA replication in simian virus 40 (SV40) chromosomes. DNA synthesis in this system required the presence of purified SV40 large tumor antigen, SV40 chromosomes prepared from virus-infected monkey cells, a crude extract from HeLa cells, and several low-molecular-weight...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4820-4829.1990
更新日期:1990-10-01 00:00:00
abstract::Marek's disease virus DNA appears to be wound around a central structure connecting the two inner poles of the capsid. Based on electron micrographs of Marek's disease virions, a diagram of spatial configuration of virus DNA is presented. This diagram may explain the pleomorphic character of the herpesvirus core obser...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1148-1150.1974
更新日期:1974-05-01 00:00:00
abstract::We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082-3087, 1998). We now extend that analysis to show that additional passag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.49-56.2000
更新日期:2000-01-01 00:00:00
abstract::Although in vitro replication of the hepatitis C virus (HCV) JFH1 clone of genotype 2a (HCVcc) has been developed, a robust cell culture system for the 1a and 1b genotypes, which are the most prevalent viruses in the world and resistant to interferon therapy, has not yet been established. As a surrogate virus system, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00608-07
更新日期:2007-08-01 00:00:00
abstract::Upon cell entry, the genomes of herpes simplex virus type 1 (HSV-1) and adenovirus (Ad) associate with distinct nuclear structures termed ND10 or promyelocytic leukemia (PML) nuclear bodies (NBs). PML NB morphology is altered or disrupted by specific viral proteins as replication proceeds. We examined whether adeno-as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.389-398.2004
更新日期:2004-01-01 00:00:00
abstract::Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-12
更新日期:2012-08-01 00:00:00
abstract::The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1394-1402.1998
更新日期:1998-02-01 00:00:00
abstract::CCCTC-binding factor (CTCF) has been implicated in various aspects of viral and host chromatin organization and transcriptional control. We showed previously that CTCF binds to a cluster of three sites in the first intron of the Kaposi's sarcoma-associated herpesvirus (KSHV) multicistronic latency-associated transcrip...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02283-12
更新日期:2013-02-01 00:00:00
abstract::Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8195-8203.1997
更新日期:1997-11-01 00:00:00
abstract::The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3441-3448.1995
更新日期:1995-06-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) is the causative agent for Kaposi sarcoma (KS), primary effusion lymphoma (PEL), and a subset of multicentric Castleman disease (MCD). The KSHV life cycle has two principal gene repertoires, latent and lytic. KSHV viral interleukin-6 (vIL-6), an analog of human ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01192-15
更新日期:2015-10-21 00:00:00
abstract::Poliovirus-infected cells undergo an increase in cytoplasmic calcium concentrations from the 4th h postinfection. The protein responsible for this effect was identified by the expression of different poliovirus nonstructural proteins in HeLa cells by using a recombinant vaccinia virus system. Synthesis of protein 2BC ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.6214-6217.1997
更新日期:1997-08-01 00:00:00
abstract::Recently, novel H7N9 influenza viruses have caused an unprecedented outbreak in humans. Pigs are an important intermediate host for influenza; thus, we assessed the replication ability of three human H7N9 viruses (A/Anhui/1/2013, A/Shanghai/1/2013, A/Shanghai/2/2013) in swine tissue explants. All viruses tested replic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02499-13
更新日期:2013-11-01 00:00:00
abstract::The HIV-1 Rev response element (RRE) is a 351-base element in unspliced and partially spliced viral RNA; binding of the RRE by the viral Rev protein induces nuclear export of RRE-containing RNAs, as required for virus replication. It contains one long, imperfect double helix (domain I), one branched domain (domain II)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00746-17
更新日期:2017-10-13 00:00:00
abstract::One-step growth determinations were performed with five strains of rotavirus in HepG2, a cell line derived from human liver. Three virus strains (SA11-C13, SA11-C14, and RRV) replicated in HepG2 cells and attained yields 10- to 100-fold above input titers. Two virus strains (B223 and SA11-4F) failed to replicate above...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1044-1049.1990
更新日期:1990-03-01 00:00:00
abstract::Walleye dermal sarcoma virus (WDSV) is a complex retrovirus associated with dermal sarcomas in walleye fish. A WDSV accessory gene encodes a cyclin homolog or retroviral cyclin (rv-cyclin). WDSV rv-cyclin was found to be associated with transcription complexes and to affect transcription in a cell-type and promoter-de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01425-06
更新日期:2006-12-01 00:00:00