Quantitative analysis of the hepatitis C virus replication complex.

abstract：:Engineered alphavirus vectors expressing reporters of infection have been used for a number of years due to their relatively low costs for analysis of virus replication and the capacity to utilize imaging systems for longitudinal measurements of growth within single animals. In general, these vectors have been derived...

journal_title：Journal of virology

authors： Sun C,Gardner CL,Watson AM,Ryman KD,Klimstra WB

更新日期：2014-02-01 00:00:00

abstract：:We have previously shown that the herpes simplex virus immediate-early regulatory protein ICP27 acts posttranscriptionally to affect mRNA processing (R. M. Sandri-Goldin and G. E. Mendoza, Genes Dev. 6:848-863, 1992). Specifically, in the presence of ICP27, spliced target mRNAs were decreased 5- to 10-fold in transfec...

journal_title：Journal of virology

authors： Hardwicke MA,Sandri-Goldin RM

更新日期：1994-08-01 00:00:00

abstract：:Current L1 virus-like particle (VLP) vaccines provide type-restricted protection against a small subset of the human papillomavirus (HPV) genotypes associated with cervical cancer, necessitating continued cytologic screening of vaccinees. Cervical cancer is most problematic in countries that lack the resources for scr...

journal_title：Journal of virology

authors： Gambhira R,Jagu S,Karanam B,Gravitt PE,Culp TD,Christensen ND,Roden RB

更新日期：2007-11-01 00:00:00

abstract：:Recognition of virus infections by pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), retinoic acid-inducible gene I (RIG-I), and melanoma differentiation associated gene 5 (MDA5), activates signaling pathways, leading to the induction of inflammatory cytokines that limit viral replication. To d...

journal_title：Journal of virology

authors： Guo H,Jiang D,Ma D,Chang J,Dougherty AM,Cuconati A,Block TM,Guo JT

更新日期：2009-01-01 00:00:00

abstract：:Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the ...

journal_title：Journal of virology

authors： Komoto S,Kugita M,Sasaki J,Taniguchi K

更新日期：2008-07-01 00:00:00

abstract：:The poliovirus (PV) genome was manipulated by replacing its 2A-encoding sequence with the corresponding sequence of coxsackie B4 virus (CBV4) or human rhinovirus type 2 (HRV2). In vitro translation of the resulting chimeric PV genomes revealed a normal cis-cleavage activity for both heterologous 2A(pro) proteinases in...

journal_title：Journal of virology

authors： Lu HH,Li X,Cuconati A,Wimmer E

更新日期：1995-12-01 00:00:00

abstract：:The expression strategy of parvoviruses of the Densovirus genus has as yet not been reported. Clones were obtained from the densonucleosis virus of Galleria mellonella (GmDNV) that yielded infectious virus upon transfection into LD652 cells. Its genome was found to be the longest (6,039 nucleotides [nt]), with the lar...

journal_title：Journal of virology

authors： Tijssen P,Li Y,El-Far M,Szelei J,Letarte M,Zádori Z

更新日期：2003-10-01 00:00:00

abstract：:Rickard's strain of feline leukemia virus (FeLV) contains two large glycoproteins and five smaller polypeptides of molecular weights 100,000 (gp >/= 100), 70,000 (gp70), 30,000 (p30), 21,000 (p21), 15,000 (p15), 11,200 (p11), and 10,000 (p10) when chromatographed on 6% agarose in the presence of 6 M guanidine hydrochl...

journal_title：Journal of virology

authors： Graves DC,Velicer LF

更新日期：1974-08-01 00:00:00

abstract：:Adenovirus infection and expression of E1A induces both proliferation and apoptosis, the latter of which is blocked by the adenovirus Bcl-2 homologue E1B 19K. The mechanism of apoptosis induction and the role that it plays in productive infection are not known. Unlike apoptosis mediated by death receptors, infection w...

journal_title：Journal of virology

authors： Cuconati A,Degenhardt K,Sundararajan R,Anschel A,White E

更新日期：2002-05-01 00:00:00

abstract：:The attachment of lymphocytic choriomeningitis virus (LCMV) to murine and primate cell lines was quantitated by a fluorescence-activated cell sorter assay in which binding of biotinylated virus was detected with streptavidin-fluorescein isothiocyanate. Cell lines that were readily infected by LCMV (e.g., MC57, Rin, BH...

journal_title：Journal of virology

authors： Borrow P,Oldstone MB

更新日期：1992-12-01 00:00:00

abstract：:GS-8374 is a potent HIV protease inhibitor (PI) with a unique diethyl-phosphonate moiety. Due to a balanced contribution of enthalpic and entropic components to its interaction with the protease (PR) active site, the compound retains activity against HIV mutants with high-level multi-PI resistance. We report here the ...

journal_title：Journal of virology

authors： Stray KM,Callebaut C,Glass B,Tsai L,Xu L,Müller B,Kräusslich HG,Cihlar T

更新日期：2013-01-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a cytoplasmic receptor/transcription factor that modulates several cellular and immunological processes following activation by pathogen-associated stimuli, though its role during virus infection is largely unknown. Here, we show that AhR is activated in cells infected with mouse...

journal_title：Journal of virology

authors： Grunewald ME,Shaban MG,Mackin SR,Fehr AR,Perlman S

更新日期：2020-01-17 00:00:00

abstract：:The assembly and budding of Sindbis virus, a prototypic member of the alphavirus subgroup in the family Togaviridae, requires a specific interaction between the nucleocapsid core and the membrane-embedded glycoproteins E1 and E2. These glycoproteins are modified posttranslationally by the addition of palmitic acid, an...

journal_title：Journal of virology

authors： Ivanova L,Schlesinger MJ

更新日期：1993-05-01 00:00:00

abstract：:Cylindrical projections surrounding the fivefold-symmetry axes in minute virus of mice (MVM) harbor central pores that penetrate through the virion shell. In newly released DNA-containing particles, these pores contain residues 28 to 38 belonging to a single copy of VP2, disposed so that its extreme N-terminal domain ...

journal_title：Journal of virology

authors： Farr GA,Cotmore SF,Tattersall P

更新日期：2006-01-01 00:00:00

abstract：:Little is known about the biological significance of human adenovirus type 5 (Ad5) E1A in vivo. However, Ad5 E1A is well defined in vitro and can be detected frequently in the lungs of patients with pulmonary disease. Transgenic expression of the Ad5 E1A gene targeted to the mouse lung reveals distinct biological effe...

journal_title：Journal of virology

authors： Yang Y,McKerlie C,Borenstein SH,Lu Z,Schito M,Chamberlain JW,Buchwald M

更新日期：2002-09-01 00:00:00

abstract：:A radioimmunoassay (RIA) has been used to measure the quantity of protein P27 (mol wt 27,000; group specific-1 [gs-1]) of avian leukoviruses in different types of chicken embryos and tissues of adult chickens. The RIA used was 200- to 300-fold more sensitive than the complement fixation test and was able to detect as ...

journal_title：Journal of virology

authors： Chen JH,Hanafusa H

更新日期：1974-02-01 00:00:00

abstract：:Skin fibroblast cultures were established from eight individuals. These cell cultures, together with WI-38 cells, were examined for susceptibility to transformation by SV40 virus. Four transformation-susceptible cell lines (TS), established from patients with Down's syndrome, were found to be three to four times more ...

journal_title：Journal of virology

authors： Potter CW,Potter AM,Oxford JS

更新日期：1970-03-01 00:00:00

abstract：:The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activi...

journal_title：Journal of virology

authors： Poranen MM,Koivunen MR,Bamford DH

更新日期：2008-09-01 00:00:00

abstract：:The human polyomavirus JC virus (JCV) establishes persistent infections in most individuals and is the etiologic agent of progressive multifocal leukoencephalopathy. In this report, we describe the establishment of a soluble cell-free system that is capable of replicating exogenous plasmid DNA containing the JCV origi...

journal_title：Journal of virology

authors： Nesper J,Smith RW,Kautz AR,Sock E,Wegner M,Grummt F,Nasheuer HP

更新日期：1997-10-01 00:00:00

abstract：:Hepatitis A virus (HAV), an atypical member of the Picornaviridae, grows poorly in cell culture. To define determinants of HAV growth, we introduced a blasticidin (Bsd) resistance gene into the virus genome and selected variants that grew at high concentrations of Bsd. The mutants grew fast and had increased rates of ...

journal_title：Journal of virology

authors： Konduru K,Kaplan GG

更新日期：2010-08-01 00:00:00

abstract：:The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...

journal_title：Journal of virology

authors： Weaver EA,Lu Z,Camacho ZT,Moukdar F,Liao HX,Ma BJ,Muldoon M,Theiler J,Nabel GJ,Letvin NL,Korber BT,Hahn BH,Haynes BF,Gao F

更新日期：2006-07-01 00:00:00

abstract：:To dissect mechanisms of caprine arthritis-encephalitis lentivirus-induced arthritis, an undefined immunodominant viral glycoprotein, gp90 (G. C. Johnson, A. F. Barbet, P. Klevjer-Anderson, and T. C. McGuire, Infect. Immun. 41:657-665, 1983), was characterized. Monoclonal antibody to gp90 and specific antiserum to env...

journal_title：Journal of virology

authors： McGuire TC,Knowles DP Jr,Davis WC,Brassfield AL,Stem TA,Cheevers WP

更新日期：1992-05-01 00:00:00

abstract：:The Ebola virus envelope glycoprotein (GP) derived from the pathogenic Zaire subtype mediates cell rounding and detachment from the extracellular matrix in 293T cells. In this study we provide evidence that GPs from the other pathogenic subtypes, Sudan and Côte d'Ivoire, as well as from Reston, a strain thought to be ...

journal_title：Journal of virology

authors： Simmons G,Wool-Lewis RJ,Baribaud F,Netter RC,Bates P

更新日期：2002-03-01 00:00:00

abstract：:Adenovirus type 2 (Ad2) and Ad5 enter epithelial cells via the coxsackievirus B Ad receptor (CAR) and alpha(v) integrin coreceptors. In the absence of CAR, they can be directed to the Fcgamma receptor 1 of hematopoietic cells by an adaptor comprising the extracellular CAR domain and the Fc portion of a human immunoglo...

journal_title：Journal of virology

authors： Meier O,Gastaldelli M,Boucke K,Hemmi S,Greber UF

更新日期：2005-02-01 00:00:00

abstract：:The membrane-proximal external region (MPER) of the human immunodeficiency virus (HIV) envelope glycoprotein (gp41) is critical for viral fusion and infectivity and is the target of three of the five known broadly neutralizing HIV type 1 (HIV-1) antibodies, 2F5, Z13, and 4E10. Here, we report the crystal structure of ...

journal_title：Journal of virology

authors： Pejchal R,Gach JS,Brunel FM,Cardoso RM,Stanfield RL,Dawson PE,Burton DR,Zwick MB,Wilson IA

更新日期：2009-09-01 00:00:00

abstract：:Nidoviruses (arteriviruses, coronaviruses, and roniviruses) are a phylogenetically compact but diverse group of positive-strand RNA viruses that includes important human and animal pathogens. Nidovirus RNA synthesis is mediated by a cytoplasmic membrane-associated replication/transcription complex that includes up to ...

journal_title：Journal of virology

authors： Nedialkova DD,Ulferts R,van den Born E,Lauber C,Gorbalenya AE,Ziebuhr J,Snijder EJ

更新日期：2009-06-01 00:00:00

abstract：:The Tax transactivator protein of human T-cell leukemia virus type 1 (HTLV-1) plays a central role in the activation of viral gene expression. In addition, Tax is capable of activating the expression of specific cellular genes and is involved in the transformation of T-lymphocytes resulting in the development of adult...

journal_title：Journal of virology

authors： Bex F,Murphy K,Wattiez R,Burny A,Gaynor RB

更新日期：1999-01-01 00:00:00

abstract：:Amino acid analysis of [3H]proline-labeled polyomavirus major capsid protein VP1 by two-dimensional paper chromatography of the acid-hydrolyzed protein revealed the presence of 3H-labeled hydroxyproline. Addition of the proline analog L-azetidine-2-carboxylic acid to infected mouse kidney cell cultures prevented or gr...

journal_title：Journal of virology

authors： Ludlow JW,Consigli RA

更新日期：1989-06-01 00:00:00

abstract：:White spot syndrome virus (WSSV) is a crustacean-infecting, double-stranded DNA virus and is the most serious viral pathogen in the global shrimp industry. WSSV is the sole recognized member of the family Nimaviridae, and the lack of genomic data on other nimaviruses has obscured the evolutionary history of WSSV. Here...

journal_title：Journal of virology

authors： Kawato S,Shitara A,Wang Y,Nozaki R,Kondo H,Hirono I

更新日期：2019-01-17 00:00:00

abstract：:The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...

journal_title：Journal of virology

authors： Tindle RW,Herd K,Doan T,Bryson G,Leggatt GR,Lambert P,Frazer IH,Street M

更新日期：2001-07-01 00:00:00