Abstract:
:The poliovirus (PV) genome was manipulated by replacing its 2A-encoding sequence with the corresponding sequence of coxsackie B4 virus (CBV4) or human rhinovirus type 2 (HRV2). In vitro translation of the resulting chimeric PV genomes revealed a normal cis-cleavage activity for both heterologous 2A(pro) proteinases in the chimeric PV polyproteins. However, only the genome containing the 2A-encoding sequence of CBV4 (PV/CBV4-2A) yielded viable virus in transfected cells, producing a mixture of large and small plaques on HeLa cell monolayers. The large-plaque variants were found to contain single-amino-acid mutations at a specific site near the C terminus of the CBV4 2A(pro) protein. When the same single-amino-acid mutations were directly introduced into the parental PV/CBV4-2A genome, chimeric viruses with a large-plaque phenotype and a wild-type PV-like growth pattern were obtained upon transfection, an observation demonstrating that these point mutations alone had a drastic effect on the growth of the PV/CBV4 chimeric virus. On the other hand, the chimeric genome containing the 2A-encoding sequence of HRV2 (PV/HRV2-2A) produced a null phenotype in transfected HeLa cells, although low-level replication of this chimeric genome was evident. We conclude that only 2A(pro) of the more closely related enterovirus CBV4 is able to functionally substitute for that of PV in vivo, and a subtle genetic modification of the CBV4 2A(pro) protein results in a drastic improvement in the growth of the chimeric PV/CBV4-2A virus. In addition, this chimeric cDNA approach enabled us to dissect multiple biological functions encoded by the 2A(pro) proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lu HH,Li X,Cuconati A,Wimmer Edoi
10.1128/JVI.69.12.7445-7452.1995subject
Has Abstractpub_date
1995-12-01 00:00:00pages
7445-52issue
12eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Initiation of reverse transcription of human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end of a cellular tRNA complexed to the primer binding site (PBS) located near the 5' end of the viral RNA genome. Although the PBSs for all naturally occurring HIV-1 viruses are complementary to the 3'-t...
journal_title:Journal of virology
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更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.61.2.516-525.1987
更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4914-4923.1995
更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1125-1132.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.275-284.1986
更新日期:1986-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.00515-11
更新日期:2011-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.869-886.1971
更新日期:1971-12-01 00:00:00
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更新日期:1993-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5196-5202.1991
更新日期:1991-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.1.280-284.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8310-8317.1996
更新日期:1996-12-01 00:00:00
abstract::To evaluate the possibility of producing transducible replication-defective hepadnaviruses, cloned mutant duck hepatitis B virus genomes were tested both for virus antigen production and viral DNA synthesis following transfection into the human hepatoma cell line HuH7. Deletion of a cis-acting 12-nucleotide sequence i...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00