Abstract:
:Hepatitis A virus (HAV), an atypical member of the Picornaviridae, grows poorly in cell culture. To define determinants of HAV growth, we introduced a blasticidin (Bsd) resistance gene into the virus genome and selected variants that grew at high concentrations of Bsd. The mutants grew fast and had increased rates of RNA replication and translation but did not produce significantly higher virus yields. Nucleotide sequence analysis and reverse genetic studies revealed that a T6069G change resulting in a F42L amino acid substitution in the viral polymerase (3D(pol)) was required for growth at high Bsd concentrations whereas a silent C7027T mutation enhanced the growth rate. Here, we identified a novel determinant(s) in 3D(pol) that controls the kinetics of HAV growth.
journal_name
J Viroljournal_title
Journal of virologyauthors
Konduru K,Kaplan GGdoi
10.1128/JVI.01470-09subject
Has Abstractpub_date
2010-08-01 00:00:00pages
8342-7issue
16eissn
0022-538Xissn
1098-5514pii
JVI.01470-09journal_volume
84pub_type
杂志文章abstract::Following the initial detection of viral infection, innate immune responses trigger the induction of numerous interferon-stimulated genes (ISGs) to inhibit virus replication and dissemination. One such ISG encodes cholesterol-25-hydroxylase (CH25H), an enzyme that catalyzes the oxidation of cholesterol to form a solub...
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journal_title:Journal of virology
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doi:10.1128/JVI.9.3.423-430.1972
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pub_type: 杂志文章
doi:10.1128/JVI.65.4.1823-1828.1991
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.6.3.280-285.1970
更新日期:1970-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.77.22.12033-12047.2003
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pub_type: 杂志文章
doi:10.1128/JVI.32.2.692-696.1979
更新日期:1979-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6967-6972.1996
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract::In July 1991, an influenza A virus, designated A/Maryland/12/91 (A/MD), was isolated from the bronchial secretions of a 27-year-old animal caretaker. He had been admitted to the hospital with bilateral pneumonia and died of acute respiratory distress syndrome 13 days later. Antigenic analyses with postinfection ferret...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.243-248.1978
更新日期:1978-05-01 00:00:00
abstract::Human metapneumovirus (hMPV), a recently described paramyxovirus, is a major etiological agent for lower respiratory tract disease in young children that can manifest with severe cough, bronchiolitis, and pneumonia. The hMPV fusion glycoprotein (F) shares conserved functional domains with other paramyxovirus F protein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.16.10678-10689.2005
更新日期:2005-08-01 00:00:00