Abstract:
:Polyamine depletion produced by exogenous arginine in Escherichia coliK-12 cultures defective in agmatine ureohydrolase activity resulted in a marked inhibition of the rates of growth and nucleic acid synthesis. Addition of putrescine or spermidine to such depleted cultures restored the control rate of growth and nucleic acid accumulation. The omission of lysine resulted in a further decrease in the rates of growth and nucleic acid synthesis in polyamine-depleted cells. The addition of exogenous cadaverine increased the rates of growth and ribonucleic acid synthesis to those observed in lysine-supplemented cultures, suggesting that lysine or a derivative of lysine serves a function similar to cadaverine. Addition of lysine to polyamine-depleted cultures at neutral pH results in the synthesis of cadaverine and a new spermidine analogue, both containing lysine carbon. This new metabolite has been isolated and identified as N-3-aminopropyl-1, 5-diaminopentane. T4D infection of the polyamine-depleted mutant resulted in a very low rate of DNA synthesis and phage maturation. The addition of putrescine or spermidine 15 min before infection restored phage DNA synthesis and phage maturation to control rates, i.e., rates observed in infected cells grown in the absence of arginine.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dion AS,Cohen SSdoi
10.1128/JVI.9.3.423-430.1972subject
Has Abstractpub_date
1972-03-01 00:00:00pages
423-30issue
3eissn
0022-538Xissn
1098-5514journal_volume
9pub_type
杂志文章abstract::The human papillomavirus type 11 (HPV-11) L1 major capsid protein can be trypsinized to generate recombinant capsomeres that retain HPV genotype-restricted capsid antigenicity (M. Li, T. P. Cripe, P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997). In the present study, HPV-11 virion-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6151-6154.1998
更新日期:1998-07-01 00:00:00
abstract::The development of neoplasia frequently involves inactivation of the p53 and retinoblastoma (Rb) tumor suppressor pathways and disruption of cell cycle checkpoints that monitor the integrity of replication and cell division. The human papillomavirus type 16 (HPV-16) oncoproteins, E6 and E7, have been shown to bind p53...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.975-985.1998
更新日期:1998-02-01 00:00:00
abstract::The inhibition of protein synthesis by cycloheximide markedly stimulated adenovirus 2 early transcription (assayed in vivo and in vitro in nuclei) when the drug was added 3 h after infection of HeLa cells. The stimulation was not uniform but ranged from three- to eightfold for region 1A to 10- to 30-fold for region 2 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.683-692.1983
更新日期:1983-02-01 00:00:00
abstract::Gammaherpesvirus 68 (gammaHV68, or MHV68) is a naturally occurring rodent pathogen that replicates to high titer in cell culture and is amenable to in vivo experimental evaluation of viral and host determinants of gammaherpesvirus disease. However, the inability of MHV68 to transform primary murine B cells in culture,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02689-07
更新日期:2008-08-01 00:00:00
abstract::The dynamics of human immunodeficiency virus type 1 (HIV-1) transcription was analyzed in vitro and in vivo by using a specific molecular approach which allows accurate quantitation of the different classes of viral mRNAs. Unspliced (US) and multiply spliced (MS) HIV-1 transcripts were assayed by competitive reverse t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7603-7613.1996
更新日期:1996-11-01 00:00:00
abstract::Although the herpes simplex virus type 1 (HSV-1) tegument is comprised of a large number of viral and cellular proteins, how and where in the cell these proteins are recruited into the virus structure is poorly understood. We have shown previously that the immediate-early gene product ICP0 is packaged by a mechanism d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00126-10
更新日期:2010-05-01 00:00:00
abstract::We report the discovery and analysis of an endogenous foamy virus (PSFVaye) within the genome of the aye-aye (Daubentonia madagascariensis), a strepsirrhine primate from Madagascar. Phylogenetic analyses indicate that PSFVaye is divergent from all known simian foamy viruses, suggesting an association between foamy vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00650-12
更新日期:2012-07-01 00:00:00
abstract::Numerous RNA viruses generate subgenomic mRNAs (sgRNAs) for expression of their 3'-proximal genes. A major step in control of viral gene expression is the regulation of sgRNA synthesis by specific promoter elements. We used barley yellow dwarf virus (BYDV) as a model system to study transcriptional control in a virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.13.5988-5996.2000
更新日期:2000-07-01 00:00:00
abstract::From purified linear and superhelical DNAs, the restriction endonuclease maps of four xenotropic murine leukemia virus DNAs from NFS, NZB, BALB/c, and AKR mice were determined with ten restriction endonucleases. Each xenotropic proviral DNA was found to be a unique restriction endonuclease map, with differences in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.963-970.1981
更新日期:1981-12-01 00:00:00
abstract::Many replication events are involved in the influenza A virus life cycle, and they are accomplished by different virus proteins with specific functions. However, because the size of the influenza virus genome is limited, the virus uses different mechanisms to express multiple viral proteins from a single gene segment....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02656-12
更新日期:2013-03-01 00:00:00
abstract::The relative contributions of interleukin-12 (IL-12) and IL-23 to viral pathogenesis have not been extensively studied. IL-12p40 mRNA rapidly increases after neurotropic coronavirus infection. Infection of mice defective in both IL-12 and IL-23 (p40(-/-)), in IL-12 alone (p35(-/-)), and in IL-23 alone (p19(-/-)) revea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00315-09
更新日期:2009-06-01 00:00:00
abstract::A nucleotide phosphohydrolase is firmly associated with a purified polyhedral cytoplasmic deoxyvirus, frog virus 3. This adenosine triphosphatase is distinguishable from known mammalian cell adenosine triphosphatases and from adenosine triphosphatase of an unrelated cytoplasmic replicating virus grown in the same host...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.5.619-624.1971
更新日期:1971-05-01 00:00:00
abstract::Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-07
更新日期:2008-01-01 00:00:00
abstract::Transcription of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) is activated through binding of the viral Tat protein to the trans-activating response (TAR) element at the 5' end of the nascent transcript. Whereas HIV type 1 (HIV-1) TAR folds a simple hairpin structure, the corresponding do...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00530-08
更新日期:2008-09-01 00:00:00
abstract::Hepatitis E virus (HEV), a positive-strand RNA virus, is an important causative agent of waterborne hepatitis. Expression of cDNA (encoding amino acids 1 to 979 of HEV nonstructural open reading frame 1) in insect cells resulted in synthesis of a 110-kDa protein (P110), a fraction of which was proteolytically processe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.14.6249-6255.2001
更新日期:2001-07-01 00:00:00
abstract::Sindbis virus (SINV) is an alphavirus that causes infection of neurons and encephalomyelitis in adult immunocompetent mice. Recovery can occur without apparent neurological damage. To better define the factors facilitating noncytolytic clearance of SINV in different regions of the central nervous system (CNS) and the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01152-06
更新日期:2007-06-01 00:00:00
abstract::Interleukin-15 (IL-15), natural killer (NK) cells, and NK T (NKT) cells, components of the innate immune system, are known to contribute to defense against pathogens, including viruses. Here we report that IL-15(-/-) (NK(-) and NKT(-/+)) mice and RAG-2(-/-)/gamma(c)(-/-) (NK(-) and NKT(-)) mice that lack all lymphoid ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::Each influenza season, a set of wild-type viruses, representing one H1N1, one H3N2, and one to two influenza B isolates, are selected for inclusion in the annual seasonal influenza vaccine. In order to develop broadly reactive subtype-specific influenza vaccines, a methodology called computationally optimized broadly ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01581-17
更新日期:2017-11-30 00:00:00
abstract:UNLABELLED:Susceptibility to alphavirus infection is age dependent, and host maturation is associated with decreased virus replication and less severe encephalitis. To identify factors associated with maturation-dependent restriction of virus replication, we studied AP-7 rat olfactory bulb neuronal cells, which can dif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02394-14
更新日期:2015-01-01 00:00:00
abstract::Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.8.787-792.1968
更新日期:1968-08-01 00:00:00
abstract:UNLABELLED:CD4+ T cells play a pivotal role in the control of chronic viral infections. Recently, nontraditional CD4+ T cell functions beyond helper effects have been described, and a role for cytolytic CD4+ T cells in the control of HIV infection has been suggested. We define here the transcriptional, phenotypic, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00438-15
更新日期:2015-08-01 00:00:00
abstract::The simian virus 40 (SV40) core origin of replication consists of three functional domains. The sequence 5'-CACTACTTCTGGAATAG-3' with an imperfect inverted repeat (underlined), a palindrome with four 5'-GAGGC-3' pentanucleotide repeats, and a 17-base-pair A + T-rich segment. We have been able to assign primary functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.509-518.1990
更新日期:1990-02-01 00:00:00
abstract::Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.5.1148-1157.1975
更新日期:1975-05-01 00:00:00
abstract::The effect of human cytomegalovirus infection on cellular DNA synthesis in human fibroblasts was measured by fluorometry and by incorporation of radiolabeled thymidine. The results show that although HCMV infection stimulates cellular DNA synthesis in both quiescent and serum-stimulated cells, radiolabeled thymidine i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6402-6404.1996
更新日期:1996-09-01 00:00:00
abstract::The herpesvirus tegument is a layer of viral and cellular proteins located between the capsid and envelope of the virion. The VP1/2 tegument protein is critical for the propagation of all herpesviruses examined. Using an infectious clone of the alphaherpesvirus pseudorabies virus, we have made a collection of truncati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01184-06
更新日期:2006-12-01 00:00:00
abstract::The envelope glycoproteins of the human immunodeficiency virus (HIV) type 1 are synthesized as a precursor molecule, gp160, which is cleaved to generate the two mature envelope glycoproteins, gp120 and gp41. The cleavage reaction, which is mediated by a host protease, occurs at a sequence highly conserved in retrovira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4670-4675.1989
更新日期:1989-11-01 00:00:00
abstract::Foamy viruses (FVs) generate their Pol protein precursor molecule independently of the Gag protein from a spliced mRNA. This mode of expression raises the question of the mechanism of Pol protein incorporation into the viral particle (capsid). We previously showed that the packaging of (pre)genomic RNA is essential fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7005-7013.2005
更新日期:2005-06-01 00:00:00
abstract::The DNase/alkaline exonuclease (AE) genes are well conserved in all herpesvirus families, but recent studies have shown that the AE proteins of gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) exhibit an additional function which shuts down host protein synthesis. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01946-07
更新日期:2008-03-01 00:00:00
abstract::A procedure has been developed for the isolation of Newcastle disease virus (NDV) envelope proteins. The two surface glycoproteins and the non-glycosylated membrane protein were solubilized with 2% Triton X-100 and 1 m KCl. Removal of the KCl by dialysis yielded by precipitation a pure preparation of the non-glycosyla...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.263-271.1973
更新日期:1973-02-01 00:00:00
abstract::The temperature-sensitive defects of virus mutants isolated from L cells persistently infected with Newcastle disease virus (NDV) were analyzed. Genetic grouping of the mutants by complementation tests was attempted by using several different methods, including yield analysis, RNA synthesis, and heterozygote formation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.472-480.1973
更新日期:1973-09-01 00:00:00