Abstract:
:The simian virus 40 (SV40) core origin of replication consists of three functional domains. The sequence 5'-CACTACTTCTGGAATAG-3' with an imperfect inverted repeat (underlined), a palindrome with four 5'-GAGGC-3' pentanucleotide repeats, and a 17-base-pair A + T-rich segment. We have been able to assign primary functions to each domain. Remarkably, SV40 large T antigen melted the inverted repeat domain in the complete absence of other origin sequences. Presumably, this protein-DNA interaction initiates a replication bubble that leads to daughter strand DNA synthesis. The pentanucleotide domain alone docked and arranged T antigen at the origin. The A + T-rich domain had no independent function, but, in the presence of the other two domains, allowed bound T antigen to extend the replication bubble. Thus, three domains of the origin coordinate the binding, melting, and DNA helicase activities of T antigen in an ordered sequence of events to initiate DNA replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Parsons R,Anderson ME,Tegtmeyer Pdoi
10.1128/JVI.64.2.509-518.1990subject
Has Abstractpub_date
1990-02-01 00:00:00pages
509-18issue
2eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.221-224.1987
更新日期:1987-01-01 00:00:00
abstract::Interleukin-10 (IL-10) suppresses the maturation and cytokine production of dendritic cells (DCs), key regulators of adaptive immunity, and prevents the activation and polarization of naïve T cells towards protective gamma interferon-producing effectors. We hypothesized that human cytomegalovirus (HCMV) utilizes its v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8720-8731.2004
更新日期:2004-08-01 00:00:00
abstract::The Vpr accessory gene product of human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus is believed to play a role in permitting entry of the viral core into the nucleus of nondividing cells. A second role for Vpr was recently suggested by Rogel et al. (M. E. Rogel, L. I. Wu, and M. Emerman, J. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6705-6711.1995
更新日期:1995-11-01 00:00:00
abstract::Wild-type herpes simplex virus 1 (HSV-1) multiplies, spreads, and rapidly destroys cells of the murine central nervous system (CNS). In contrast, mutants lacking both copies of the gamma(1)34.5- gene have been shown to be virtually lacking in virulence even after direct inoculation of high-titered virus into the CNS o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5560-5569.1997
更新日期:1997-07-01 00:00:00
abstract::Human T-cell leukemia virus type I (HTLV-I)-infected T-cell lines constitutively produce high levels of biologically active lymphotoxin (LT; tumor necrosis factor-beta) protein and LT mRNA. To understand the regulation of LT transcription by HTLV-I, we analyzed the ability of a series of deletions of the LT promoter t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5412-5419.1990
更新日期:1990-11-01 00:00:00
abstract::Vesicular stomatitis virus has the potential for very rapid evolution in the laboratory, but like many other arboviruses, it evolves at a relatively slow rate in the natural environment. Previous work showed that alternating replication in different cell types does not promote stasis. In order to determine whether oth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12236-12242.2004
更新日期:2004-11-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) does not replicate in murine cells. We investigated the basis of this block by infecting a murine NIH 3T3 reporter cell line that stably expressed human CD4, CCR5, and cyclin T1 and contained a transactivatable HIV-1 long terminal repeat (LTR)-green fluorescent protein (GFP)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3859-3870.2000
更新日期:2000-04-01 00:00:00
abstract::Recombinant Epstein-Barr viruses (EBV) with a translation termination codon mutation inserted into the nuclear protein 3A (EBNA-3A) or 3C (EBNA-3C) open reading frame were generated by second-site homologous recombination. These mutant viruses were used to infect primary B lymphocytes to assess the requirement of EBNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2014-2025.1993
更新日期:1993-04-01 00:00:00
abstract::Processes by which human herpesviruses penetrate and are released from polarized epithelial cells, which have distinct apical and basolateral membrane domains differing in protein and lipid content, are poorly understood. We recently reported that human cytomegalovirus (CMV) mutants with deletions of the gene US9 form...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5717-5727.1998
更新日期:1998-07-01 00:00:00
abstract::Herpes simplex virus type 1 packages its DNA genome into a precursor capsid, referred to as the procapsid. Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added subsequently. To determine whether the association of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2118-2126.2006
更新日期:2006-03-01 00:00:00
abstract::Highly pathogenic influenza A viruses (IAV) from avian hosts were first reported to directly infect humans 20 years ago. However, such infections are rare events, and our understanding of factors promoting or restricting zoonotic transmission is still limited. One accessory protein of IAV, PB1-F2, was associated with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00425-18
更新日期:2018-05-14 00:00:00
abstract::Phage 2 adsorbed to Pseudomonas aeruginosa strain BI in 5 mM Tris buffer, providing that cations like Na(+), Mg(2+), or Ca(2+) were present. Adsorption was observed over a broad pH range, reaching a maximum level around pH 7.5, which coincided with the pH required for maximal activity of the phage 2-associated slime p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.22-27.1974
更新日期:1974-01-01 00:00:00
abstract::Injection of conventional or axenic weanling mice with potent sheep or goat antibody to mouse interferon alpha/beta resulted in a decrease in the basal level of 2-5A synthetase in resting peritoneal macrophages and rendered these cells permissive for vesicular stomatitis virus. There was a good inverse correlation bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.1.221-227.1985
更新日期:1985-01-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) encodes a 40-kDa Tax phosphoprotein. Tax is a transcriptional activator which modulates expression of the viral long terminal repeat and transcription of many cellular genes. Because Tax is a critical HTLV-1 factor which mediates viral transformation of T cells during the ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.21.11686-11695.2004
更新日期:2004-11-01 00:00:00
abstract::The cocultivation of a lung cell line from the Southeast Asian mouse Mus cervicolor with cells from heterologous species has resulted in the isolation of two new distinct type C viruses. Both viruses are endogenous to M. cervicolor and are present in multiple copies in the cellular DNA of these mice. One of the viruse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.849-862.1977
更新日期:1977-03-01 00:00:00
abstract::Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02072-05
更新日期:2006-11-01 00:00:00
abstract::At mucosal sites, papillomavirus genomes can persist in the epithelial basal layer following immune-mediated regression. Subsequent T-cell depletion stimulates a 3- to 5-log increase in the viral copy number, to levels associated with productive infection. Reappearance of microlesions was rare within the short time fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02589-13
更新日期:2014-01-01 00:00:00
abstract::Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved for only a few cell lines. To establish efficient and versatile models for transmission, we developed neuroblastoma cell lines overexpressing type A mouse prion protein, MoPrP(C)-A, and then t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.320-325.2000
更新日期:2000-01-01 00:00:00
abstract::Retrovirus particles each contain two copies of the viral genome in the form of a noncovalently linked RNA dimer. Earlier studies have mapped a cis-acting region near the 5' end of the human immunodeficiency virus type 1 (HIV-1) genome, termed the psi locus, which appears essential for initiation of genomic dimerizati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3407-3414.1997
更新日期:1997-05-01 00:00:00
abstract::The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00321-13
更新日期:2013-09-01 00:00:00
abstract::While epidermal growth factor receptor (EGFR) has been shown to be important in the entry process for multiple viruses, including hepatitis C virus (HCV), the molecular mechanisms by which EGFR facilitates HCV entry are not well understood. Using the infectious cell culture HCV model (HCVcc), we demonstrate that the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00750-12
更新日期:2012-10-01 00:00:00
abstract::Newcastle disease virus (NDV) edits its P-gene mRNA by inserting a nontemplated G residue(s) at a conserved editing site (3'-UUUUUCCC-template strand). In the wild-type virus, three amino-coterminal P-gene-derived proteins, P, V, and W, are produced at frequencies of approximately 68, 29, and 2%, respectively. By appl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.420-428.2001
更新日期:2001-01-01 00:00:00
abstract::Minute virus of canines (MVC) is a member of the genus Bocavirus in the family Parvoviridae. We have molecularly cloned and sequenced the 5'- and 3'-terminal palindromes of MVC. The MVC genome, 5,404 nucleotides (nt) in length, shared an identity of 52.6% and 52.1% with that of human bocavirus and bovine parvovirus, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02569-08
更新日期:2009-04-01 00:00:00
abstract::The complex evolutionary process of human immunodeficiency virus type 1 (HIV-1) is marked by a high level of genetic variation. It has been shown that the HIV-1 genome is characterized by variable and more constant regions, unequal nucleotide frequencies, and preference for G-to-A substitutions. However, this knowledg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4761-4770.1997
更新日期:1997-06-01 00:00:00
abstract::Protein P4, an early protein of double-stranded RNA bacteriophage phi 6, is a component of the virion-associated RNA polymerase complex and possesses a nucleoside triphosphate (NTP) phosphohydrolase activity. We have produced and characterized a panel of 20 P4-specific monoclonal antibodies. Epitope mapping using trun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2879-2886.1993
更新日期:1993-05-01 00:00:00
abstract::The expression and predicted products of rat c-ros-1 gene, the proto-oncogene of v-ros in UR2 sarcoma virus, were characterized. The c-ros-1 gene was found to be expressed in a tissue-specific manner, and the sizes of its transcripts were heterogeneous: 8.2 kilobases (kb) long in lung and kidney tissues, 6.9 kb in hea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2117-2125.1990
更新日期:1990-05-01 00:00:00
abstract::Phage P22 can integrate as prophage into a recombination-deficient (Rec(-)) strain of Salmonella typhimurium. At 37 C, the integration efficiency is only 10% that in Rec(+) infection, but at 25 C the efficiencies in Rec(-) and Rec(+) hosts are similar. Rec(-) lysogens cannot be induced by ultraviolet irradiation or by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.7.702-709.1968
更新日期:1968-07-01 00:00:00
abstract::Anti-human immunodeficiency virus type 1 (HIV-1) antibodies whose binding to gp120 is enhanced by CD4 binding (CD4i antibodies) are generally considered nonneutralizing for primary HIV-1 isolates. However, a novel CD4i-specific Fab fragment, X5, has recently been found to neutralize a wide range of primary isolates. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10557-10565.2003
更新日期:2003-10-01 00:00:00
abstract::Coxsackievirus B3 (CVB3) is an enterovirus in the family Picornaviridae that is significant to human health, being associated with myocarditis, aseptic meningitis, and pancreatitis, among other conditions. In addition to humans, Sichuan snub-nosed monkeys can be infected and killed by CVB3. Here, we report the first c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02365-12
更新日期:2012-12-01 00:00:00
abstract::The retroviral Gag protein plays the central role in the assembly process and can form membrane-enclosed, virus-like particles in the absence of any other viral products. These particles are similar to authentic virions in density and size. Three small domains of the human immunodeficiency virus type 1 (HIV-1) Gag pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.6.4667-4677.1998
更新日期:1998-06-01 00:00:00