Isolation and purification of the envelope proteins of Newcastle disease virus.

abstract：:The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...

journal_title：Journal of virology

authors： Jin H,Zhou H,Lu B,Kemble G

更新日期：2004-01-01 00:00:00

abstract：:Zyxin, a focal adhesion molecule, interacts specifically with the E6 protein from human papillomavirus (HPV) type 6 in a yeast two-hybrid screen of a cDNA library prepared from human keratinocytes. Zyxin does not interact significantly with E6 proteins from HPV types 11, 16, or 18. The interaction was confirmed by in ...

journal_title：Journal of virology

authors： Degenhardt YY,Silverstein S

更新日期：2001-12-01 00:00:00

abstract：:Langat virus (LGT), the naturally attenuated member of the tick-borne encephalitis virus (TBEV) complex, was tested extensively in clinical trials as a live TBEV vaccine and was found to induce a protective, durable immune response; however, it retained a low residual neuroinvasiveness in mice and humans. In order to ...

journal_title：Journal of virology

authors： Rumyantsev AA,Murphy BR,Pletnev AG

更新日期：2006-02-01 00:00:00

abstract：UNLABELLED:Retargeting of gammaretroviral envelope proteins has shown promising results in the isolation of novel isolates with therapeutic potential. However, the optimal conditions required to obtain high-affinity retargeted envelope proteins with narrow tropism are not understood. This study highlights the advantage...

journal_title：Journal of virology

authors： Valdivieso-Torres L,Sarangi A,Whidby J,Marcotrigiano J,Roth MJ

更新日期：2015-12-30 00:00:00

abstract：:Besides its role in viral assembly, the vesicular stomatitis virus (VSV) matrix (M) protein causes cytopathic effects such as cell rounding (D. Blondel, G. G. Harmison, and M. Schubert, J. Virol. 64:1716-1725, 1990). DNA cotransfection assays demonstrated that VSV M protein was able to inhibit the transcription of a r...

journal_title：Journal of virology

authors： Paik SY,Banerjea AC,Harmison GG,Chen CJ,Schubert M

更新日期：1995-06-01 00:00:00

abstract：:A major determinant of neurovirulence for the GDVII strain of Theiler's virus, a murine picornavirus, was mapped to the P1 capsid protein region. Chimeric viruses were constructed by using sequences from the 5' noncoding and P1 regions of the virulent GDVII strain to replace equivalent regions of the less virulent BeA...

journal_title：Journal of virology

authors： Pritchard AE,Jensen K,Lipton HL

更新日期：1993-07-01 00:00:00

abstract：:Major histocompatibility complex class I (MHC I) molecules present antigenic peptides for CD8(+) T-cell recognition. Prior to cell surface expression, proper MHC I loading is conducted by the peptide-loading complex (PLC), composed of the MHC I heavy chain (HC) and β(2)-microglobulin (β(2)m), the peptide transporter T...

journal_title：Journal of virology

authors： Halenius A,Hauka S,Dölken L,Stindt J,Reinhard H,Wiek C,Hanenberg H,Koszinowski UH,Momburg F,Hengel H

更新日期：2011-04-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Nef interferes with the endocytic machinery to modulate the cell surface expression of CD4. However, the basal trafficking of CD4 is governed by different rules in the target cells of HIV-1: whereas CD4 is rapidly internalized from the cell surface in myeloid cells, CD4 is s...

journal_title：Journal of virology

authors： Laguette N,Brégnard C,Bouchet J,Benmerah A,Benichou S,Basmaciogullari S

更新日期：2009-07-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) gp41 plays a critical role in the viral fusion process, and its N- and C-terminal heptad repeat domains serve as important targets for developing anti-HIV-1 drugs, like T-20 (generic name, enfuvirtide; brand name, Fuzeon). Here, we conducted a yeast two-hybrid screening on a...

journal_title：Journal of virology

authors： Zhu Y,Lu L,Xu L,Yang H,Jiang S,Chen YH

更新日期：2010-09-01 00:00:00

abstract：:Clink, a 20-kDa protein of faba bean necrotic yellows virus, a single-stranded DNA plant virus, interacts with pRB family members and a SKP1 homologue from Medicago sativa. An LxCxE motif and an F-box of Clink mediate the interactions with the respective proteins. The capacity of Clink to bind pRB correlates with its ...

journal_title：Journal of virology

authors： Aronson MN,Meyer AD,Györgyey J,Katul L,Vetten HJ,Gronenborn B,Timchenko T

更新日期：2000-04-01 00:00:00

abstract：:Brome mosaic virus (BMV) RNA replication is directed by two virus-encoded proteins, 1a and 2a. The amino-terminal half of 1a is a distant homolog of alphavirus nonstructural protein nsP1, which has been implicated in capping viral RNAs. In this study, we examined the enzymatic activities of BMV 1a expressed in yeast, ...

journal_title：Journal of virology

authors： Ahola T,Ahlquist P

更新日期：1999-12-01 00:00:00

abstract：:Six overlapping viral RNAs are synthesized in cells infected with the avian coronavirus infectious bronchitis virus (IBV). These RNAs contain a 3'-coterminal nested sequence set and were assumed to be viral mRNAs. The seven major IBV virion proteins are all produced by processing of three polypeptides of ca. 23, 51, a...

journal_title：Journal of virology

authors： Stern DF,Sefton BM

更新日期：1984-04-01 00:00:00

abstract：:Analysis of purified simian rotavirus has shown that it contains fewer structural polypeptide classes than previously reported. Two polypeptides (molecular weights, 62,000 and 28,000) commonly found in purified rotaviruses were, in fact, produced by cleavage of a larger structural polypeptide (molecular weight, about ...

journal_title：Journal of virology

authors： Espejo RT,López S,Arias C

更新日期：1981-01-01 00:00:00

abstract：:Breast cancer is the second leading cause of cancer-related deaths in women in the United States. Triple-negative breast cancer constitutes a subset of breast cancer that is associated with higher rates of relapse, decreased survival, and limited therapeutic options for patients afflicted with this type of breast canc...

journal_title：Journal of virology

authors： Rodríguez Stewart RM,Berry JTL,Berger AK,Yoon SB,Hirsch AL,Guberman JA,Patel NB,Tharp GK,Bosinger SE,Mainou BA

更新日期：2019-11-13 00:00:00

abstract：UNLABELLED:It was recently reported that 44% of the oropharyngeal samples from the healthy humans in a study cohort had DNA sequences similar to that of the chlorovirus ATCV-1 (Acanthocystis turfacea chlorella virus 1, family Phycodnaviridae) and that these study subjects had decreases in visual processing and visual m...

journal_title：Journal of virology

authors： Petro TM,Agarkova IV,Zhou Y,Yolken RH,Van Etten JL,Dunigan DD

更新日期：2015-12-01 00:00:00

abstract：:Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...

journal_title：Journal of virology

authors： Barban V,Quérat G,Sauze N,Filippi P,Vigne R,Russo P,Vitu C

更新日期：1984-11-01 00:00:00

abstract：:Adenovirus E1B 55K protein cooperates with E1A gene products to induce cell transformation. E1B 55K mediates its effects by binding to and inhibiting the transcriptional activation and growth-suppression functions of the tumor suppressor p53. Previous studies in vivo have suggested that E1B 55K has an active role in r...

journal_title：Journal of virology

authors： Martin ME,Berk AJ

更新日期：1998-04-01 00:00:00

abstract：:Varicella-zoster virus (VZV) is renowned for its very low titer when grown in cultured cells. There remains no single explanation for the low infectivity. In this study, viral particles on the surfaces of infected cells were examined by several imaging technologies. Few surface particles were detected at 48 h postinfe...

journal_title：Journal of virology

authors： Carpenter JE,Hutchinson JA,Jackson W,Grose C

更新日期：2008-03-01 00:00:00

abstract：UNLABELLED:In order to investigate the novel function(s) of the herpes simplex virus 1 (HSV-1) immediate early protein ICP22, we screened for ICP22-binding proteins in HSV-1-infected cells. Our results were as follows. (i) Tandem affinity purification of ICP22 from infected cells, coupled with mass spectrometry-based p...

journal_title：Journal of virology

authors： Maruzuru Y,Shindo K,Liu Z,Oyama M,Kozuka-Hata H,Arii J,Kato A,Kawaguchi Y

更新日期：2014-07-01 00:00:00

abstract：:The fundamental question of whether a primed immune system is capable of preventing latent gammaherpesvirus infection remains unanswered. Recent studies showing that vaccination can reduce acute replication and short-term latency but cannot alter long-term latency further call into question the possibility of achievin...

journal_title：Journal of virology

authors： Tibbetts SA,McClellan JS,Gangappa S,Speck SH,Virgin HW 4th

更新日期：2003-02-01 00:00:00

abstract：UNLABELLED:The ATR kinase has essential functions in maintenance of genome integrity in response to replication stress. ATR is recruited to RPA-coated single-stranded DNA at DNA damage sites via its interacting partner, ATRIP, which binds to the large subunit of RPA. ATR activation typically leads to activation of the ...

journal_title：Journal of virology

authors： Adeyemi RO,Pintel DJ

更新日期：2014-09-01 00:00:00

abstract：:The single-stranded DNA containing the Moloney murine leukemia virus origin for plus-strand synthesis was cloned in M13mp2 and used as a template for avian myeloblastosis virus reverse transcriptase in the presence of Moloney RNA which had been treated with pancreatic RNase A. The RNA pieces containing the polypurine ...

journal_title：Journal of virology

authors： Champoux JJ,Gilboa E,Baltimore D

更新日期：1984-03-01 00:00:00

abstract：:Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...

journal_title：Journal of virology

authors： Kuff EL,Lueders KK,Orenstein JM,Wilson SH

更新日期：1976-08-01 00:00:00

abstract：:The replication of vaccinia virus is thought to take place exclusively in the cytoplasm of host cells. However, using DNA-DNA hybridization techniques, it can be shown that a significant fraction of the synthesis of vaccinia DNA takes place in the nucleus as well as the cytoplasm. The (3H) thymiding pulse-labeled vacc...

journal_title：Journal of virology

authors： LaColla P,Weissbach A

更新日期：1975-02-01 00:00:00

abstract：:A putative transcription factor, C/EBP, isolated from rat liver nuclei, has been shown to bind to at least two different sequence motifs: the CCAAT promoter domain and a core sequence [GTGG(T/A)(T/A)(T/A)G] common to many viral enhancers, including simian virus 40 and human hepatitis B virus. It has been proposed that...

journal_title：Journal of virology

authors： Pei DQ,Shih CH

更新日期：1990-04-01 00:00:00

abstract：:The chimpanzee is the only animal model for investigating the pathogenesis of viral hepatitis types A through E in humans. Studies of the host response, including microarray analyses, have relied on the close relationship between these two primate species: chimpanzee samples are commonly tested with human-based reagen...

journal_title：Journal of virology

authors： Yu C,Boon D,McDonald SL,Myers TG,Tomioka K,Nguyen H,Engle RE,Govindarajan S,Emerson SU,Purcell RH

更新日期：2010-11-01 00:00:00

abstract：:The initial aim of this study was to combine attributes of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad vectors. In executing our experimental strategy, we found that, in addition to the known incompatibil...

journal_title：Journal of virology

authors： Philpott NJ,Giraud-Wali C,Dupuis C,Gomos J,Hamilton H,Berns KI,Falck-Pedersen E

更新日期：2002-06-01 00:00:00

abstract：:The vaccinia virus H2R gene (VACWR 100) is conserved in all sequenced members of the poxvirus family and encodes a protein with a predicted transmembrane domain and four invariant cysteines. A recombinant vaccinia virus, in which expression of the H2 protein is stringently regulated, was unable to replicate without in...

journal_title：Journal of virology

authors： Senkevich TG,Moss B

更新日期：2005-04-01 00:00:00

abstract：:Yaba tumor poxvirus deoxyribonucleic acid (DNA) has a density of 1.6905 in CsCl and its T(m) value in 0.015 m citrate in saline is 82.3. The guanine plus cytosine content estimated from these properties was taken to be 32.5 +/- 0.5%, a value 2 to 3% less than for DNA from other poxviruses reported to date. ...

journal_title：Journal of virology

authors： Yohn DS,Gallagher JF

更新日期：1969-02-01 00:00:00

abstract：:Resistance to human immunodeficiency virus type 1 protease (HIV PR) inhibitors results primarily from the selection of multiple mutations in the protease region. Because many of these mutations are selected for the ability to decrease inhibitor binding in the active site, they also affect substrate binding and potenti...

journal_title：Journal of virology

authors： Dauber DS,Ziermann R,Parkin N,Maly DJ,Mahrus S,Harris JL,Ellman JA,Petropoulos C,Craik CS

更新日期：2002-02-01 00:00:00